Minichromosome maintenance protein 2 is a reliable proliferative marker in breast carcinoma

Reena Rahayu Md Zain, Mokhtar Mastura, Md Ali Siti-Aishah, Md Ali Munirah, Norlia Abdullah, Ibrahim Naqiyah, Rohaizak Muhammad, Noor Akmal Sharifah

Research output: Contribution to journalArticle

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Abstract

This is a study aimed to examine the distribution pattern of a specific minichromosome maintenance protein 2 (MCM2) in benign and malignant breast tissue. We also aim to correlate the frequency of expression of MCM2 with the degree of tumor differentiation. We used immunohistochemistry to examine the distribution and expression pattern of MCM2 on formalin-fixed paraffin-embedded tissue sections of benign (n = 30) and malignant breast tissue (n = 70) (IDC 56, DCIS 4, ILC 2, nonductal 4, mixed type 4). We quantified MCM2 expression by calculating a labeling index, which represents the percentage of epithelial nuclei that stained positively. Immunoreactivity was heterogenous in all the 70 malignant cases examined. Epithelial cells in cycle are most frequent at the tumor periphery. Labeling index of MCM2 was greatest in grade 3 (poorly differentiated) and lowest in grade 1 tumors (well differentiated). Minichromosome maintenance protein 2 expression in breast cancer showed a positive association with histologic grade (P < .05). In all the benign breast tissue examined, no proliferating compartments could be characterized. Minichromosome maintenance protein 2 is a useful proliferative marker of breast carcinoma. The frequency of expression of MCM2 showed an inverse correlation with the degree of tumor differentiation.

Original languageEnglish
Pages (from-to)340-343
Number of pages4
JournalAnnals of Diagnostic Pathology
Volume12
Issue number5
DOIs
Publication statusPublished - Oct 2008

Fingerprint

Minichromosome Maintenance Proteins
Breast Neoplasms
Breast
Neoplasms
Carcinoma, Intraductal, Noninfiltrating
Paraffin
Formaldehyde
Cell Cycle
Epithelial Cells
Immunohistochemistry

Keywords

  • Breast carcinoma
  • Minichromosome maintenance protein 2 (Mcm2)
  • Proliferative marker

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Minichromosome maintenance protein 2 is a reliable proliferative marker in breast carcinoma. / Md Zain, Reena Rahayu; Mastura, Mokhtar; Siti-Aishah, Md Ali; Munirah, Md Ali; Abdullah, Norlia; Naqiyah, Ibrahim; Muhammad, Rohaizak; Sharifah, Noor Akmal.

In: Annals of Diagnostic Pathology, Vol. 12, No. 5, 10.2008, p. 340-343.

Research output: Contribution to journalArticle

Md Zain, Reena Rahayu ; Mastura, Mokhtar ; Siti-Aishah, Md Ali ; Munirah, Md Ali ; Abdullah, Norlia ; Naqiyah, Ibrahim ; Muhammad, Rohaizak ; Sharifah, Noor Akmal. / Minichromosome maintenance protein 2 is a reliable proliferative marker in breast carcinoma. In: Annals of Diagnostic Pathology. 2008 ; Vol. 12, No. 5. pp. 340-343.
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