Microscopy-based techniques: A reliable method for detecting Blastocystis sp. Infection

Samseh Abdullah Noradilah, Norhayati Moktar, Tengku Shahrul Anuar, Ii Li Lee, Fatmah Md Salleh, Siti Nor Azreen Abdul Manap, Noor Shazleen Husnie Mohd Mohtar, Syed Muhamad Azrul, Wan Omar Abdullah, Anisah Nordin, Syamsa Rizal Abdullah

Research output: Contribution to journalArticle

Abstract

Microscopy technique is widely used in the detection of Blastocystis sp. infection. This study was performed to compare two microscopy-based techniques; in vitro cultivation of faecal specimens in Jones' medium (IVC) followed by Wheatley Trichrome staining and fixed-PVA faecal specimens (PVA) followed by Wheatley Trichrome staining with single-round polymerase chain reaction (PCR) as the reference technique. Examination was performed on 466 faecal samples obtained from Orang Asli community in Pahang, Malaysia. Detection rate of Blastocystis sp. was highest in IVC (35.6%) followed by PVA (20.0%). Single-round PCR detected 41.0% of the faecal specimens. The sensitivity and specificity of PVA and IVC compared to the reference technique were 75.3% (95% CI: 65.2-83.6) and 68.5% (CI: 63.7-73.3), and 88.6% (CI: 82.7-93.0) and 86.3% (CI: 81.9-90.0) respectively. The agreement between the reference technique and PVA showed fair statistically significant agreement by Cohen Kappa statistics of (K=0.318, p<0.001), meanwhile substantial statistically significant agreement was observed between PCR and IVC by Cohen Kappa (K=0.727, p<0.001). Therefore, in vitro cultivation in Jones' medium followed by Wheatley Trichrome staining of faecal specimens should be used as a screening technique in the detection Blastocystis sp. infections.

Original languageEnglish
Pages (from-to)149-153
Number of pages5
JournalSylwan
Volume161
Issue number6
Publication statusPublished - 1 Jan 2017

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Blastocystis
polymerase chain reaction
microscopy
infection
methodology
Malaysia
method
detection
statistics
screening

ASJC Scopus subject areas

  • Forestry

Cite this

Noradilah, S. A., Moktar, N., Anuar, T. S., Lee, I. L., Salleh, F. M., Manap, S. N. A. A., ... Abdullah, S. R. (2017). Microscopy-based techniques: A reliable method for detecting Blastocystis sp. Infection. Sylwan, 161(6), 149-153.

Microscopy-based techniques : A reliable method for detecting Blastocystis sp. Infection. / Noradilah, Samseh Abdullah; Moktar, Norhayati; Anuar, Tengku Shahrul; Lee, Ii Li; Salleh, Fatmah Md; Manap, Siti Nor Azreen Abdul; Mohtar, Noor Shazleen Husnie Mohd; Azrul, Syed Muhamad; Abdullah, Wan Omar; Nordin, Anisah; Abdullah, Syamsa Rizal.

In: Sylwan, Vol. 161, No. 6, 01.01.2017, p. 149-153.

Research output: Contribution to journalArticle

Noradilah, SA, Moktar, N, Anuar, TS, Lee, IL, Salleh, FM, Manap, SNAA, Mohtar, NSHM, Azrul, SM, Abdullah, WO, Nordin, A & Abdullah, SR 2017, 'Microscopy-based techniques: A reliable method for detecting Blastocystis sp. Infection', Sylwan, vol. 161, no. 6, pp. 149-153.
Noradilah SA, Moktar N, Anuar TS, Lee IL, Salleh FM, Manap SNAA et al. Microscopy-based techniques: A reliable method for detecting Blastocystis sp. Infection. Sylwan. 2017 Jan 1;161(6):149-153.
Noradilah, Samseh Abdullah ; Moktar, Norhayati ; Anuar, Tengku Shahrul ; Lee, Ii Li ; Salleh, Fatmah Md ; Manap, Siti Nor Azreen Abdul ; Mohtar, Noor Shazleen Husnie Mohd ; Azrul, Syed Muhamad ; Abdullah, Wan Omar ; Nordin, Anisah ; Abdullah, Syamsa Rizal. / Microscopy-based techniques : A reliable method for detecting Blastocystis sp. Infection. In: Sylwan. 2017 ; Vol. 161, No. 6. pp. 149-153.
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AU - Lee, Ii Li

AU - Salleh, Fatmah Md

AU - Manap, Siti Nor Azreen Abdul

AU - Mohtar, Noor Shazleen Husnie Mohd

AU - Azrul, Syed Muhamad

AU - Abdullah, Wan Omar

AU - Nordin, Anisah

AU - Abdullah, Syamsa Rizal

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