MicroRNA (miRNA) expression profiling of peripheral blood samples in multiple myeloma patients using microarray

Yusnita Yusnita, M. D. Norsiah, I. Zakiah, K. M. Chang, V. S. Purushotaman, Z. Zubaidah, A. Rahman A. Jamal

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

suggesting their important role in the pathogenesis of human cancers. Multiple myeloma (MM) is a cancer of plasma cells, the third most common cancer of the blood after lymphoma and leukaemia. There are several published reports on miRNAs in MM, however most used bone marrow rather than peripheral blood samples. The aim of this study is to characterise miRNA expression in normal and MM patients using peripheral blood samples as it is less invasive and is readily available from patients. Blood samples from 35 MM patients were analysed using the microarray method. We identified up-regulation of 36 miRNAs (57%) and down-regulation of 27 miRNAs (43%). We also identified the CCND2, HMGA2 and IGF1R genes were among the highly predictive target genes (PCT> 0.80) for most of the deregulated miRNAs. These genes are known to play important roles in MM as well as other cancers. Five miRNAs (let-7c, miR-16, miR- 449, miR-181a and miR-181b) were found to exhibit similar expression patterns (p < 0.05) in peripheral blood when compared to data obtained by using bone marrow aspirates from MM patients in other studies. In conclusion, our study has demonstrated that miRNAs are also present and differentially expressed in the peripheral blood of MM patients compared to controls and may potentially serve as candidate tumour biomarkers in MM. In particular, let-7c and miR-16 have been shown to be significantly expressed in the bone marrow.

Original languageEnglish
Pages (from-to)133-143
Number of pages11
JournalMalaysian Journal of Pathology
Volume34
Issue number2
Publication statusPublished - Dec 2012

Fingerprint

Multiple Myeloma
MicroRNAs
Bone Marrow
Neoplasms
Genes
Tumor Biomarkers
Plasma Cells
Lymphoma
Leukemia
Up-Regulation
Down-Regulation

Keywords

  • Biomarker
  • MicroRNA
  • MiRNA expression
  • Multiple myeloma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Cell Biology
  • Histology

Cite this

Yusnita, Y., Norsiah, M. D., Zakiah, I., Chang, K. M., Purushotaman, V. S., Zubaidah, Z., & A. Jamal, A. R. (2012). MicroRNA (miRNA) expression profiling of peripheral blood samples in multiple myeloma patients using microarray. Malaysian Journal of Pathology, 34(2), 133-143.

MicroRNA (miRNA) expression profiling of peripheral blood samples in multiple myeloma patients using microarray. / Yusnita, Yusnita; Norsiah, M. D.; Zakiah, I.; Chang, K. M.; Purushotaman, V. S.; Zubaidah, Z.; A. Jamal, A. Rahman.

In: Malaysian Journal of Pathology, Vol. 34, No. 2, 12.2012, p. 133-143.

Research output: Contribution to journalArticle

Yusnita, Y, Norsiah, MD, Zakiah, I, Chang, KM, Purushotaman, VS, Zubaidah, Z & A. Jamal, AR 2012, 'MicroRNA (miRNA) expression profiling of peripheral blood samples in multiple myeloma patients using microarray', Malaysian Journal of Pathology, vol. 34, no. 2, pp. 133-143.
Yusnita Y, Norsiah MD, Zakiah I, Chang KM, Purushotaman VS, Zubaidah Z et al. MicroRNA (miRNA) expression profiling of peripheral blood samples in multiple myeloma patients using microarray. Malaysian Journal of Pathology. 2012 Dec;34(2):133-143.
Yusnita, Yusnita ; Norsiah, M. D. ; Zakiah, I. ; Chang, K. M. ; Purushotaman, V. S. ; Zubaidah, Z. ; A. Jamal, A. Rahman. / MicroRNA (miRNA) expression profiling of peripheral blood samples in multiple myeloma patients using microarray. In: Malaysian Journal of Pathology. 2012 ; Vol. 34, No. 2. pp. 133-143.
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