Micropropagation of ginger (Zingiber officinalevar. rubrum) using buds from microshoots

A. R. Zuraida, M. A. Mohd Shukri, M. N. Erny Sabrina, O. Ayu Nazreena, Che Radziah Che Mohd. Zain, G. Pavallekoodi, S. Sreeramanan

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Zingiber officinalevar. Rubrum(ZOR) is cultivated for its medicinal value despite the constraints of longer life cycle. The study has established an efficient and reproducible protocol to micropropagate ZOR using buds generated on the surface of the ginger. Surface sterilized young buds of 0.5-1 cm and 2-4 cm cultured on Murashige and Skoog (MS) supplemented with BAP showed the highest survival rate (55-65%) and produced the highest average number of microshoots per explant (3.2±0.06) respectively. MS medium supplemented with different concentrations and combinations of auxin and cytokinin were used to evaluate shoot multiplication and root induction. BAP concentrations between 3.0-5.0 mg/L was very effective in promoting microshoots and resulted in 100% of microshoot propagation. Microshoots cultured on MS medium supplemented with 3 mg/L BAP and 0.5 mg/L NAA produced the highest number of shoots while 0-0.5 mg/L BAP enhanced shoot length and 3mg/L NAA in combination with BAP produced highest number of roots. Microshoots maintained on MS medium supplemented with 4.5% sucrose produced the highest number of plantlets (23±2.5) and roots per explants (15.4±2.4) meanwhile reducing the length of lateral roots (2.6±0.2).

Original languageEnglish
Pages (from-to)1153-1158
Number of pages6
JournalPakistan Journal of Botany
Volume48
Issue number3
Publication statusPublished - 1 Jun 2016

Fingerprint

Zingiber
ginger
micropropagation
buds
naphthaleneacetic acid
shoots
explants
cytokinins
plantlets
auxins
life cycle (organisms)
survival rate
sucrose

Keywords

  • Carbon source
  • Micropropagation
  • Plant growth regulator
  • Young bud
  • Zingiber officinale

ASJC Scopus subject areas

  • Plant Science

Cite this

Zuraida, A. R., Mohd Shukri, M. A., Erny Sabrina, M. N., Ayu Nazreena, O., Che Mohd. Zain, C. R., Pavallekoodi, G., & Sreeramanan, S. (2016). Micropropagation of ginger (Zingiber officinalevar. rubrum) using buds from microshoots. Pakistan Journal of Botany, 48(3), 1153-1158.

Micropropagation of ginger (Zingiber officinalevar. rubrum) using buds from microshoots. / Zuraida, A. R.; Mohd Shukri, M. A.; Erny Sabrina, M. N.; Ayu Nazreena, O.; Che Mohd. Zain, Che Radziah; Pavallekoodi, G.; Sreeramanan, S.

In: Pakistan Journal of Botany, Vol. 48, No. 3, 01.06.2016, p. 1153-1158.

Research output: Contribution to journalArticle

Zuraida, AR, Mohd Shukri, MA, Erny Sabrina, MN, Ayu Nazreena, O, Che Mohd. Zain, CR, Pavallekoodi, G & Sreeramanan, S 2016, 'Micropropagation of ginger (Zingiber officinalevar. rubrum) using buds from microshoots', Pakistan Journal of Botany, vol. 48, no. 3, pp. 1153-1158.
Zuraida AR, Mohd Shukri MA, Erny Sabrina MN, Ayu Nazreena O, Che Mohd. Zain CR, Pavallekoodi G et al. Micropropagation of ginger (Zingiber officinalevar. rubrum) using buds from microshoots. Pakistan Journal of Botany. 2016 Jun 1;48(3):1153-1158.
Zuraida, A. R. ; Mohd Shukri, M. A. ; Erny Sabrina, M. N. ; Ayu Nazreena, O. ; Che Mohd. Zain, Che Radziah ; Pavallekoodi, G. ; Sreeramanan, S. / Micropropagation of ginger (Zingiber officinalevar. rubrum) using buds from microshoots. In: Pakistan Journal of Botany. 2016 ; Vol. 48, No. 3. pp. 1153-1158.
@article{0a5740b7a9b249d68f00e7e4d31c37c8,
title = "Micropropagation of ginger (Zingiber officinalevar. rubrum) using buds from microshoots",
abstract = "Zingiber officinalevar. Rubrum(ZOR) is cultivated for its medicinal value despite the constraints of longer life cycle. The study has established an efficient and reproducible protocol to micropropagate ZOR using buds generated on the surface of the ginger. Surface sterilized young buds of 0.5-1 cm and 2-4 cm cultured on Murashige and Skoog (MS) supplemented with BAP showed the highest survival rate (55-65{\%}) and produced the highest average number of microshoots per explant (3.2±0.06) respectively. MS medium supplemented with different concentrations and combinations of auxin and cytokinin were used to evaluate shoot multiplication and root induction. BAP concentrations between 3.0-5.0 mg/L was very effective in promoting microshoots and resulted in 100{\%} of microshoot propagation. Microshoots cultured on MS medium supplemented with 3 mg/L BAP and 0.5 mg/L NAA produced the highest number of shoots while 0-0.5 mg/L BAP enhanced shoot length and 3mg/L NAA in combination with BAP produced highest number of roots. Microshoots maintained on MS medium supplemented with 4.5{\%} sucrose produced the highest number of plantlets (23±2.5) and roots per explants (15.4±2.4) meanwhile reducing the length of lateral roots (2.6±0.2).",
keywords = "Carbon source, Micropropagation, Plant growth regulator, Young bud, Zingiber officinale",
author = "Zuraida, {A. R.} and {Mohd Shukri}, {M. A.} and {Erny Sabrina}, {M. N.} and {Ayu Nazreena}, O. and {Che Mohd. Zain}, {Che Radziah} and G. Pavallekoodi and S. Sreeramanan",
year = "2016",
month = "6",
day = "1",
language = "English",
volume = "48",
pages = "1153--1158",
journal = "Pakistan Journal of Botany",
issn = "0556-3321",
publisher = "Pakistan Botanical Society",
number = "3",

}

TY - JOUR

T1 - Micropropagation of ginger (Zingiber officinalevar. rubrum) using buds from microshoots

AU - Zuraida, A. R.

AU - Mohd Shukri, M. A.

AU - Erny Sabrina, M. N.

AU - Ayu Nazreena, O.

AU - Che Mohd. Zain, Che Radziah

AU - Pavallekoodi, G.

AU - Sreeramanan, S.

PY - 2016/6/1

Y1 - 2016/6/1

N2 - Zingiber officinalevar. Rubrum(ZOR) is cultivated for its medicinal value despite the constraints of longer life cycle. The study has established an efficient and reproducible protocol to micropropagate ZOR using buds generated on the surface of the ginger. Surface sterilized young buds of 0.5-1 cm and 2-4 cm cultured on Murashige and Skoog (MS) supplemented with BAP showed the highest survival rate (55-65%) and produced the highest average number of microshoots per explant (3.2±0.06) respectively. MS medium supplemented with different concentrations and combinations of auxin and cytokinin were used to evaluate shoot multiplication and root induction. BAP concentrations between 3.0-5.0 mg/L was very effective in promoting microshoots and resulted in 100% of microshoot propagation. Microshoots cultured on MS medium supplemented with 3 mg/L BAP and 0.5 mg/L NAA produced the highest number of shoots while 0-0.5 mg/L BAP enhanced shoot length and 3mg/L NAA in combination with BAP produced highest number of roots. Microshoots maintained on MS medium supplemented with 4.5% sucrose produced the highest number of plantlets (23±2.5) and roots per explants (15.4±2.4) meanwhile reducing the length of lateral roots (2.6±0.2).

AB - Zingiber officinalevar. Rubrum(ZOR) is cultivated for its medicinal value despite the constraints of longer life cycle. The study has established an efficient and reproducible protocol to micropropagate ZOR using buds generated on the surface of the ginger. Surface sterilized young buds of 0.5-1 cm and 2-4 cm cultured on Murashige and Skoog (MS) supplemented with BAP showed the highest survival rate (55-65%) and produced the highest average number of microshoots per explant (3.2±0.06) respectively. MS medium supplemented with different concentrations and combinations of auxin and cytokinin were used to evaluate shoot multiplication and root induction. BAP concentrations between 3.0-5.0 mg/L was very effective in promoting microshoots and resulted in 100% of microshoot propagation. Microshoots cultured on MS medium supplemented with 3 mg/L BAP and 0.5 mg/L NAA produced the highest number of shoots while 0-0.5 mg/L BAP enhanced shoot length and 3mg/L NAA in combination with BAP produced highest number of roots. Microshoots maintained on MS medium supplemented with 4.5% sucrose produced the highest number of plantlets (23±2.5) and roots per explants (15.4±2.4) meanwhile reducing the length of lateral roots (2.6±0.2).

KW - Carbon source

KW - Micropropagation

KW - Plant growth regulator

KW - Young bud

KW - Zingiber officinale

UR - http://www.scopus.com/inward/record.url?scp=84973664405&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84973664405&partnerID=8YFLogxK

M3 - Article

VL - 48

SP - 1153

EP - 1158

JO - Pakistan Journal of Botany

JF - Pakistan Journal of Botany

SN - 0556-3321

IS - 3

ER -