Micropropagation of Citrus halimii - An endangered species of South-east Asia

Normah Mohd. Noor, S. Hamidah, F. D. Ghani

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

A successful system of direct organogenesis is described for the wild citrus tree, Citrus halimii Stone which used in vitro seedling explants cultured on Murashige and Skoog medium supplemented with 0.4-11.1 μM 6-benzyladenine. Hypocotyl was the best explant for multiple shoots regeneration. Maximum number of shoots was obtained on medium with 2.2-11.1 μM 6-benzyladenine. Rooting of regenerated shoots was best on Murashige and Skoog medium supplemented with 2.7 μM α-naphthalenacetic acid. In vitro and ex vitro rooted plantlets survived (on average 83.3%) after being transferred to the soil mixture consisting of soil, sand and organic material (1: 1: 1) and kept in the glasshouse.

Original languageEnglish
Pages (from-to)225-227
Number of pages3
JournalPlant Cell, Tissue and Organ Culture
Volume50
Issue number3
DOIs
Publication statusPublished - 1997

Fingerprint

N-benzyladenine
Endangered Species
Far East
Citrus
micropropagation
South East Asia
endangered species
Soil
Hypocotyl
benzyladenine
shoots
Organogenesis
explants
Seedlings
Regeneration
organogenesis
hypocotyls
Acids
plantlets
rooting

Keywords

  • Citrus
  • Ex vitro establishment
  • Rooting
  • Shoot regeneration

ASJC Scopus subject areas

  • Plant Science
  • Biotechnology

Cite this

Micropropagation of Citrus halimii - An endangered species of South-east Asia. / Mohd. Noor, Normah; Hamidah, S.; Ghani, F. D.

In: Plant Cell, Tissue and Organ Culture, Vol. 50, No. 3, 1997, p. 225-227.

Research output: Contribution to journalArticle

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