Micronucleated erythrocytes as an assay to assess actions by physical and chemical genotoxic agents in Clarias gariepinus

Ismail B. Bahari, Fauziah M. Noor, Nik Mat Daud

    Research output: Contribution to journalArticle

    50 Citations (Scopus)

    Abstract

    An in vivo study on the effects of both physical (γ-radiation) and chemical (mitomycin C) genotoxic agents was carried out with the catfish species, Clarias gariepinus. The fish were either exposed to γ-radiation at doses of 0-9 Gy or injected intraperitoneally with mitomycin C at concentrations of 0-2 mg/kg. Micronucleated erythrocytes were sampled from 0 to 60 days post treatment. Data obtained showed a time-dependent response of the induction of micronucleated erythrocytes with both genotoxic agents. A linear dose-dependent increase was observed 2-4 days after treatment. These data show the importance of sampling time in the micronucleus assay with Clarias gariepinus.

    Original languageEnglish
    Pages (from-to)1-5
    Number of pages5
    JournalMutation Research/Environmental Mutagenesis and Related Subjects
    Volume313
    Issue number1
    DOIs
    Publication statusPublished - 1994

    Fingerprint

    Pharmacologic Actions
    Mitomycin
    Assays
    Erythrocytes
    Radiation
    Catfishes
    Micronucleus Tests
    Fish
    Dosimetry
    Fishes
    Sampling

    Keywords

    • Catfish (Clarias gariepinus)
    • Micronucleus assay
    • Sampling time

    ASJC Scopus subject areas

    • Genetics
    • Toxicology

    Cite this

    Micronucleated erythrocytes as an assay to assess actions by physical and chemical genotoxic agents in Clarias gariepinus. / Bahari, Ismail B.; Noor, Fauziah M.; Mat Daud, Nik.

    In: Mutation Research/Environmental Mutagenesis and Related Subjects, Vol. 313, No. 1, 1994, p. 1-5.

    Research output: Contribution to journalArticle

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