Microfluidic dielectrophoretic cell manipulation towards stable cell contact assemblies

Mohd Anuar Md Ali, Aminuddin Bin Ahmad Kayani, Leslie Y. Yeo, Adam F. Chrimes, Muhammad Zamharir Ahmad, Kostya (Ken) Ostrikov, Burhanuddin Yeop Majlis

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Cell contact formation, which is the process by which cells are brought into close proximity is an important biotechnological process in cell and molecular biology. Such manipulation is achieved by various means, among which dielectrophoresis (DEP) is widely used due to its simplicity. Here, we show the advantages in the judicious choice of the DEP microelectrode configuration in terms of limiting undesirable effects of dielectric heating on the cells, which could lead to their inactivation or death, as well as the possibility for cell clustering, which is particularly advantageous over the linear cell chain arrangement typically achieved to date with DEP. This study comprises of experimental work as well as mathematical modeling using COMSOL. In particular, we establish the parameters in a capillary-based microfluidic system giving rise to these optimum cell–cell contact configurations, together with the possibility for facilitating other cell manipulations such as spinning and rotation, thus providing useful protocols for application into microfluidic bioparticle manipulation systems for diagnostics, therapeutics or for furthering research in cellular bioelectricity and intercellular interactions.

Original languageEnglish
Article number95
JournalBiomedical Microdevices
Volume20
Issue number4
DOIs
Publication statusPublished - 1 Dec 2018

Fingerprint

Microfluidics
Electrophoresis
Contacts (fluid mechanics)
Bioelectric phenomena
Dielectric heating
Cytology
Molecular biology
Microelectrodes
Heating
Cluster Analysis
Cell Biology
Molecular Biology
Research

Keywords

  • Cell chain
  • Cell contact
  • Dielectrophoresis
  • Microfluidics
  • Rotation
  • Spinning

ASJC Scopus subject areas

  • Biomedical Engineering
  • Molecular Biology

Cite this

Md Ali, M. A., Kayani, A. B. A., Yeo, L. Y., Chrimes, A. F., Ahmad, M. Z., Ostrikov, K. K., & Yeop Majlis, B. (2018). Microfluidic dielectrophoretic cell manipulation towards stable cell contact assemblies. Biomedical Microdevices, 20(4), [95]. https://doi.org/10.1007/s10544-018-0341-1

Microfluidic dielectrophoretic cell manipulation towards stable cell contact assemblies. / Md Ali, Mohd Anuar; Kayani, Aminuddin Bin Ahmad; Yeo, Leslie Y.; Chrimes, Adam F.; Ahmad, Muhammad Zamharir; Ostrikov, Kostya (Ken); Yeop Majlis, Burhanuddin.

In: Biomedical Microdevices, Vol. 20, No. 4, 95, 01.12.2018.

Research output: Contribution to journalArticle

Md Ali, Mohd Anuar ; Kayani, Aminuddin Bin Ahmad ; Yeo, Leslie Y. ; Chrimes, Adam F. ; Ahmad, Muhammad Zamharir ; Ostrikov, Kostya (Ken) ; Yeop Majlis, Burhanuddin. / Microfluidic dielectrophoretic cell manipulation towards stable cell contact assemblies. In: Biomedical Microdevices. 2018 ; Vol. 20, No. 4.
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