Microbiological assessment and evaluation of rehydration instructions on powdered infant formulas, follow-up formulas, and infant foods in Malaysia

Norrakiah Abdullah Sani, S. H P Hartantyo, S. J. Forsythe

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A total of 90 samples comprising powdered infant formulas (n=51), follow-up formulas (n=21), and infant foods (n=18) from 15 domestic and imported brands were purchased from various retailers in Klang Valley, Malaysia and evaluated in terms of microbiological quality and the similarity of rehydration instructions on the product label to guidelines set by the World Health Organization. Microbiological analysis included the determination of aerobic plate count (APC) and the presence of Enterobacteriaceae and Cronobacter spp. Isolates of interest were identified using ID 32E (bioMérieux France, Craponne, France). In this study, 87% of powdered infant formulas, follow-up formulas, and infant foods analyzed had an APC below the permitted level of <104cfu/g. These acceptable APC ranged between <102 to 7.2×103cfu/g. The most frequently isolated Enterobacteriaceae was Enterobacter cloacae, which was present in 3 infant formulas and 1 infant food tested. Other Enterobacteriaceae detected from powdered infant and follow-up formulas were Citrobacter spp., Klebsiella spp., and other Enterobacter spp. No Cronobacter species were found in any samples. Rehydration instructions from the product labels were collated and it was observed that none directed the use of water with a temperature >70°C for formula preparation, as specified by the 2008 revised World Health Organization guidelines. Six brands instructed the use of water at 40 to 55°C, a temperature range that would support the survival and even growth of Enterobacteriaceae.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalJournal of Dairy Science
Volume96
Issue number1
DOIs
Publication statusPublished - Jan 2013

Fingerprint

Infant Food
Infant Formula
infant foods
Fluid Therapy
Malaysia
World Health Organization
infant formulas
rehydration
Enterobacteriaceae
plate count
France
Cronobacter
Guidelines
product labeling
microbiological quality
valleys
Temperature
Water
Growth
temperature

Keywords

  • Follow-up formula
  • Infant foods
  • Powdered infant formula
  • Rehydration instructions

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Food Science
  • Genetics

Cite this

@article{10afeb63e2cc4d0ea7140fd7dead2b87,
title = "Microbiological assessment and evaluation of rehydration instructions on powdered infant formulas, follow-up formulas, and infant foods in Malaysia",
abstract = "A total of 90 samples comprising powdered infant formulas (n=51), follow-up formulas (n=21), and infant foods (n=18) from 15 domestic and imported brands were purchased from various retailers in Klang Valley, Malaysia and evaluated in terms of microbiological quality and the similarity of rehydration instructions on the product label to guidelines set by the World Health Organization. Microbiological analysis included the determination of aerobic plate count (APC) and the presence of Enterobacteriaceae and Cronobacter spp. Isolates of interest were identified using ID 32E (bioM{\'e}rieux France, Craponne, France). In this study, 87{\%} of powdered infant formulas, follow-up formulas, and infant foods analyzed had an APC below the permitted level of <104cfu/g. These acceptable APC ranged between <102 to 7.2×103cfu/g. The most frequently isolated Enterobacteriaceae was Enterobacter cloacae, which was present in 3 infant formulas and 1 infant food tested. Other Enterobacteriaceae detected from powdered infant and follow-up formulas were Citrobacter spp., Klebsiella spp., and other Enterobacter spp. No Cronobacter species were found in any samples. Rehydration instructions from the product labels were collated and it was observed that none directed the use of water with a temperature >70°C for formula preparation, as specified by the 2008 revised World Health Organization guidelines. Six brands instructed the use of water at 40 to 55°C, a temperature range that would support the survival and even growth of Enterobacteriaceae.",
keywords = "Follow-up formula, Infant foods, Powdered infant formula, Rehydration instructions",
author = "{Abdullah Sani}, Norrakiah and Hartantyo, {S. H P} and Forsythe, {S. J.}",
year = "2013",
month = "1",
doi = "10.3168/jds.2012-5409",
language = "English",
volume = "96",
pages = "1--8",
journal = "Journal of Dairy Science",
issn = "0022-0302",
publisher = "Elsevier Limited",
number = "1",

}

TY - JOUR

T1 - Microbiological assessment and evaluation of rehydration instructions on powdered infant formulas, follow-up formulas, and infant foods in Malaysia

AU - Abdullah Sani, Norrakiah

AU - Hartantyo, S. H P

AU - Forsythe, S. J.

PY - 2013/1

Y1 - 2013/1

N2 - A total of 90 samples comprising powdered infant formulas (n=51), follow-up formulas (n=21), and infant foods (n=18) from 15 domestic and imported brands were purchased from various retailers in Klang Valley, Malaysia and evaluated in terms of microbiological quality and the similarity of rehydration instructions on the product label to guidelines set by the World Health Organization. Microbiological analysis included the determination of aerobic plate count (APC) and the presence of Enterobacteriaceae and Cronobacter spp. Isolates of interest were identified using ID 32E (bioMérieux France, Craponne, France). In this study, 87% of powdered infant formulas, follow-up formulas, and infant foods analyzed had an APC below the permitted level of <104cfu/g. These acceptable APC ranged between <102 to 7.2×103cfu/g. The most frequently isolated Enterobacteriaceae was Enterobacter cloacae, which was present in 3 infant formulas and 1 infant food tested. Other Enterobacteriaceae detected from powdered infant and follow-up formulas were Citrobacter spp., Klebsiella spp., and other Enterobacter spp. No Cronobacter species were found in any samples. Rehydration instructions from the product labels were collated and it was observed that none directed the use of water with a temperature >70°C for formula preparation, as specified by the 2008 revised World Health Organization guidelines. Six brands instructed the use of water at 40 to 55°C, a temperature range that would support the survival and even growth of Enterobacteriaceae.

AB - A total of 90 samples comprising powdered infant formulas (n=51), follow-up formulas (n=21), and infant foods (n=18) from 15 domestic and imported brands were purchased from various retailers in Klang Valley, Malaysia and evaluated in terms of microbiological quality and the similarity of rehydration instructions on the product label to guidelines set by the World Health Organization. Microbiological analysis included the determination of aerobic plate count (APC) and the presence of Enterobacteriaceae and Cronobacter spp. Isolates of interest were identified using ID 32E (bioMérieux France, Craponne, France). In this study, 87% of powdered infant formulas, follow-up formulas, and infant foods analyzed had an APC below the permitted level of <104cfu/g. These acceptable APC ranged between <102 to 7.2×103cfu/g. The most frequently isolated Enterobacteriaceae was Enterobacter cloacae, which was present in 3 infant formulas and 1 infant food tested. Other Enterobacteriaceae detected from powdered infant and follow-up formulas were Citrobacter spp., Klebsiella spp., and other Enterobacter spp. No Cronobacter species were found in any samples. Rehydration instructions from the product labels were collated and it was observed that none directed the use of water with a temperature >70°C for formula preparation, as specified by the 2008 revised World Health Organization guidelines. Six brands instructed the use of water at 40 to 55°C, a temperature range that would support the survival and even growth of Enterobacteriaceae.

KW - Follow-up formula

KW - Infant foods

KW - Powdered infant formula

KW - Rehydration instructions

UR - http://www.scopus.com/inward/record.url?scp=84871608845&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84871608845&partnerID=8YFLogxK

U2 - 10.3168/jds.2012-5409

DO - 10.3168/jds.2012-5409

M3 - Article

C2 - 23141821

AN - SCOPUS:84871608845

VL - 96

SP - 1

EP - 8

JO - Journal of Dairy Science

JF - Journal of Dairy Science

SN - 0022-0302

IS - 1

ER -