Microbial contamination of meat contact surfaces at the selected beef processing plants in Selangor and its biofilm formation ability

Siti Shahara Zulfakar, Nur Faizah Abu-Bakar, Anith Amirah Aidilputra, Alexander Miatong, Elizabeth Sinirisan Chong

Research output: Contribution to journalArticle

Abstract

Cross-contamination of meat products with the surrounding contact surfaces does occur during slaughtering procedures. This study aimed to assess the microbial contamination level of meat contact surfaces at the selected local abattoirs. Swab samples of knives, splitting tools and air curtains were collected from two sites in Selangor. The presence of selected indicator and pathogenic microorganisms (total Aerobic Plate Count [APC], Enterobacteriaceae, Escherichia coli [E. coli] and Salmonella spp.) were determined using the plate count method. The isolates obtained were then tested in terms of the biofilm formation ability using the microtiter plate crystal violet assay. Overall results showed that the average total APC for all contact surfaces was 4.77±1.14 log CFU/cm2 (mean±sd). Enterobacteriaceae was found on 75% of the samples at 3.31±1.14 log CFU/cm2. E. coli was only detected on 11.36% of the total contact surfaces at 2.91±1.00 log CFU/ cm2 whereas 25% of the total samples were positive with Salmonella spp. Splitting tools were identified as the most contaminated meat contact surface. Variations in biofilm formation ability were observed between isolates although most of them formed weakly adherent biofilms, especially at 4°C. The study findings help to enhance the systems used in the local abattoirs to ensure safe and top quality meat production.

Original languageEnglish
Pages (from-to)709-726
Number of pages18
JournalPertanika Journal of Tropical Agricultural Science
Volume42
Issue number2
Publication statusPublished - 1 May 2019

Fingerprint

meat processing plants
microbial contamination
biofilm
meat
plate count
Enterobacteriaceae
slaughterhouses
Salmonella
Escherichia coli
gentian violet
knives
cross contamination
meat production
meat products
sampling
slaughter
microorganisms
air
assays

Keywords

  • Abattoirs
  • Beef
  • Biofilm formation
  • Meat contact surface
  • Meat contamination

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

Microbial contamination of meat contact surfaces at the selected beef processing plants in Selangor and its biofilm formation ability. / Zulfakar, Siti Shahara; Abu-Bakar, Nur Faizah; Aidilputra, Anith Amirah; Miatong, Alexander; Chong, Elizabeth Sinirisan.

In: Pertanika Journal of Tropical Agricultural Science, Vol. 42, No. 2, 01.05.2019, p. 709-726.

Research output: Contribution to journalArticle

Zulfakar, Siti Shahara ; Abu-Bakar, Nur Faizah ; Aidilputra, Anith Amirah ; Miatong, Alexander ; Chong, Elizabeth Sinirisan. / Microbial contamination of meat contact surfaces at the selected beef processing plants in Selangor and its biofilm formation ability. In: Pertanika Journal of Tropical Agricultural Science. 2019 ; Vol. 42, No. 2. pp. 709-726.
@article{b7e297313f364f08bd26df593096f016,
title = "Microbial contamination of meat contact surfaces at the selected beef processing plants in Selangor and its biofilm formation ability",
abstract = "Cross-contamination of meat products with the surrounding contact surfaces does occur during slaughtering procedures. This study aimed to assess the microbial contamination level of meat contact surfaces at the selected local abattoirs. Swab samples of knives, splitting tools and air curtains were collected from two sites in Selangor. The presence of selected indicator and pathogenic microorganisms (total Aerobic Plate Count [APC], Enterobacteriaceae, Escherichia coli [E. coli] and Salmonella spp.) were determined using the plate count method. The isolates obtained were then tested in terms of the biofilm formation ability using the microtiter plate crystal violet assay. Overall results showed that the average total APC for all contact surfaces was 4.77±1.14 log CFU/cm2 (mean±sd). Enterobacteriaceae was found on 75{\%} of the samples at 3.31±1.14 log CFU/cm2. E. coli was only detected on 11.36{\%} of the total contact surfaces at 2.91±1.00 log CFU/ cm2 whereas 25{\%} of the total samples were positive with Salmonella spp. Splitting tools were identified as the most contaminated meat contact surface. Variations in biofilm formation ability were observed between isolates although most of them formed weakly adherent biofilms, especially at 4°C. The study findings help to enhance the systems used in the local abattoirs to ensure safe and top quality meat production.",
keywords = "Abattoirs, Beef, Biofilm formation, Meat contact surface, Meat contamination",
author = "Zulfakar, {Siti Shahara} and Abu-Bakar, {Nur Faizah} and Aidilputra, {Anith Amirah} and Alexander Miatong and Chong, {Elizabeth Sinirisan}",
year = "2019",
month = "5",
day = "1",
language = "English",
volume = "42",
pages = "709--726",
journal = "Pertanika Journal of Tropical Agricultural Science",
issn = "1511-3701",
publisher = "Universiti Putra Malaysia",
number = "2",

}

TY - JOUR

T1 - Microbial contamination of meat contact surfaces at the selected beef processing plants in Selangor and its biofilm formation ability

AU - Zulfakar, Siti Shahara

AU - Abu-Bakar, Nur Faizah

AU - Aidilputra, Anith Amirah

AU - Miatong, Alexander

AU - Chong, Elizabeth Sinirisan

PY - 2019/5/1

Y1 - 2019/5/1

N2 - Cross-contamination of meat products with the surrounding contact surfaces does occur during slaughtering procedures. This study aimed to assess the microbial contamination level of meat contact surfaces at the selected local abattoirs. Swab samples of knives, splitting tools and air curtains were collected from two sites in Selangor. The presence of selected indicator and pathogenic microorganisms (total Aerobic Plate Count [APC], Enterobacteriaceae, Escherichia coli [E. coli] and Salmonella spp.) were determined using the plate count method. The isolates obtained were then tested in terms of the biofilm formation ability using the microtiter plate crystal violet assay. Overall results showed that the average total APC for all contact surfaces was 4.77±1.14 log CFU/cm2 (mean±sd). Enterobacteriaceae was found on 75% of the samples at 3.31±1.14 log CFU/cm2. E. coli was only detected on 11.36% of the total contact surfaces at 2.91±1.00 log CFU/ cm2 whereas 25% of the total samples were positive with Salmonella spp. Splitting tools were identified as the most contaminated meat contact surface. Variations in biofilm formation ability were observed between isolates although most of them formed weakly adherent biofilms, especially at 4°C. The study findings help to enhance the systems used in the local abattoirs to ensure safe and top quality meat production.

AB - Cross-contamination of meat products with the surrounding contact surfaces does occur during slaughtering procedures. This study aimed to assess the microbial contamination level of meat contact surfaces at the selected local abattoirs. Swab samples of knives, splitting tools and air curtains were collected from two sites in Selangor. The presence of selected indicator and pathogenic microorganisms (total Aerobic Plate Count [APC], Enterobacteriaceae, Escherichia coli [E. coli] and Salmonella spp.) were determined using the plate count method. The isolates obtained were then tested in terms of the biofilm formation ability using the microtiter plate crystal violet assay. Overall results showed that the average total APC for all contact surfaces was 4.77±1.14 log CFU/cm2 (mean±sd). Enterobacteriaceae was found on 75% of the samples at 3.31±1.14 log CFU/cm2. E. coli was only detected on 11.36% of the total contact surfaces at 2.91±1.00 log CFU/ cm2 whereas 25% of the total samples were positive with Salmonella spp. Splitting tools were identified as the most contaminated meat contact surface. Variations in biofilm formation ability were observed between isolates although most of them formed weakly adherent biofilms, especially at 4°C. The study findings help to enhance the systems used in the local abattoirs to ensure safe and top quality meat production.

KW - Abattoirs

KW - Beef

KW - Biofilm formation

KW - Meat contact surface

KW - Meat contamination

UR - http://www.scopus.com/inward/record.url?scp=85067631968&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85067631968&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:85067631968

VL - 42

SP - 709

EP - 726

JO - Pertanika Journal of Tropical Agricultural Science

JF - Pertanika Journal of Tropical Agricultural Science

SN - 1511-3701

IS - 2

ER -