Microbial community analysis of mixed anaerobic microflora in suspended sludge of ASBR producing hydrogen from palm oil mill effluent

Marzieh Badiei, Jamaliah Md Jahim, Nurina Anuar, Siti Rozaimah Sheikh Abdullah, Swee Su Lim, Mohd Aidil Kamaruzzaman

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

This study investigated the microbial community of an anaerobic sequencing batch reactor (ASBR) operating at mesophilic temperature under varying hydraulic retention times (HRTs) for evaluating optimal hydrogen production conditions, using palm oil mill effluent (POME) as substrate. POME sludge enriched by heat treatment with hydrogen-producing bacteria was used as inoculum and acclimated with the POME. The microbial community was determined by first isolating cultivable bacteria at each operating HRT and then using polymerase chain reaction (PCR). The PCR products were sequenced and sequence identification was performed using the BLAST algorithm and Genbank database. The findings revealed that about 50% of the isolates present were members of the genus Streptococcus, about 30% were Lactobacillus species and around 20% were identified as species of genus Clostridium. Scanning electron microscopy (SEM) analysis also confirmed the presence of spherical and rod-shaped microbial morphologies in the sludge samples of bioreactor during prolonged cultivation.

Original languageEnglish
Pages (from-to)3169-3176
Number of pages8
JournalInternational Journal of Hydrogen Energy
Volume37
Issue number4
DOIs
Publication statusPublished - Feb 2012

Fingerprint

sludge
Palm oil
sequencing
effluents
Batch reactors
Effluents
polymerase chain reaction
oils
Polymerase chain reaction
reactors
hydraulics
Hydrogen
bacteria
Bacteria
hydrogen
inoculum
Hydraulics
Clostridium
streptococcus
bioreactors

Keywords

  • Anaerobic sequencing batch reactor (ASBR)
  • Biohydrogen
  • Microbial community
  • Palm oil mill effluent (POME)

ASJC Scopus subject areas

  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Condensed Matter Physics
  • Energy Engineering and Power Technology

Cite this

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title = "Microbial community analysis of mixed anaerobic microflora in suspended sludge of ASBR producing hydrogen from palm oil mill effluent",
abstract = "This study investigated the microbial community of an anaerobic sequencing batch reactor (ASBR) operating at mesophilic temperature under varying hydraulic retention times (HRTs) for evaluating optimal hydrogen production conditions, using palm oil mill effluent (POME) as substrate. POME sludge enriched by heat treatment with hydrogen-producing bacteria was used as inoculum and acclimated with the POME. The microbial community was determined by first isolating cultivable bacteria at each operating HRT and then using polymerase chain reaction (PCR). The PCR products were sequenced and sequence identification was performed using the BLAST algorithm and Genbank database. The findings revealed that about 50{\%} of the isolates present were members of the genus Streptococcus, about 30{\%} were Lactobacillus species and around 20{\%} were identified as species of genus Clostridium. Scanning electron microscopy (SEM) analysis also confirmed the presence of spherical and rod-shaped microbial morphologies in the sludge samples of bioreactor during prolonged cultivation.",
keywords = "Anaerobic sequencing batch reactor (ASBR), Biohydrogen, Microbial community, Palm oil mill effluent (POME)",
author = "Marzieh Badiei and {Md Jahim}, Jamaliah and Nurina Anuar and {Sheikh Abdullah}, {Siti Rozaimah} and Lim, {Swee Su} and Kamaruzzaman, {Mohd Aidil}",
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AU - Badiei, Marzieh

AU - Md Jahim, Jamaliah

AU - Anuar, Nurina

AU - Sheikh Abdullah, Siti Rozaimah

AU - Lim, Swee Su

AU - Kamaruzzaman, Mohd Aidil

PY - 2012/2

Y1 - 2012/2

N2 - This study investigated the microbial community of an anaerobic sequencing batch reactor (ASBR) operating at mesophilic temperature under varying hydraulic retention times (HRTs) for evaluating optimal hydrogen production conditions, using palm oil mill effluent (POME) as substrate. POME sludge enriched by heat treatment with hydrogen-producing bacteria was used as inoculum and acclimated with the POME. The microbial community was determined by first isolating cultivable bacteria at each operating HRT and then using polymerase chain reaction (PCR). The PCR products were sequenced and sequence identification was performed using the BLAST algorithm and Genbank database. The findings revealed that about 50% of the isolates present were members of the genus Streptococcus, about 30% were Lactobacillus species and around 20% were identified as species of genus Clostridium. Scanning electron microscopy (SEM) analysis also confirmed the presence of spherical and rod-shaped microbial morphologies in the sludge samples of bioreactor during prolonged cultivation.

AB - This study investigated the microbial community of an anaerobic sequencing batch reactor (ASBR) operating at mesophilic temperature under varying hydraulic retention times (HRTs) for evaluating optimal hydrogen production conditions, using palm oil mill effluent (POME) as substrate. POME sludge enriched by heat treatment with hydrogen-producing bacteria was used as inoculum and acclimated with the POME. The microbial community was determined by first isolating cultivable bacteria at each operating HRT and then using polymerase chain reaction (PCR). The PCR products were sequenced and sequence identification was performed using the BLAST algorithm and Genbank database. The findings revealed that about 50% of the isolates present were members of the genus Streptococcus, about 30% were Lactobacillus species and around 20% were identified as species of genus Clostridium. Scanning electron microscopy (SEM) analysis also confirmed the presence of spherical and rod-shaped microbial morphologies in the sludge samples of bioreactor during prolonged cultivation.

KW - Anaerobic sequencing batch reactor (ASBR)

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KW - Palm oil mill effluent (POME)

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