Microarray profiling of secretoryphase endometrium from patients with recurrent miscarriage

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25 Citations (Scopus)

Abstract

The aim of the present study was to identify differentially expressed genes and their related biological pathways in the secretory phase endometrium from patients with recurrent miscarriage (RM) and fertile subjects. Endometrial samples from RM and fertile patients were analyzed using the Affymetrix GeneChip® ST Array. The bioinformatic analysis using the Partek Genomic Suite revealed 346 genes that were differentially expressed (175 up-regulated and 171 down-regulated) in the endometrium of RM patients compared to the fertile subjects (fold change ≥1.5, p<0.005). Validation step using quantitative real-time polymerase chain reaction (qPCR) confirmed a similar expression pattern of four exemplary genes: one up-regulated gene (fibroblast growth factor 9, FGF9) and three down-regulated genes: integrin β3 (ITGB3), colony stimulating factor 1 (CSF1) and matrix-metalloproteinases 19 (MMP19). The Gene Set Enrichment Analysis (GSEA) and the Pathway Studio Software have found 101 signaling pathways (p<0.05) associated with the affected genes including the FGFR 3/signal transducer and activator of transcription (STAT) pathway and the CSF1R/STAT pathway. Cell adhesion, cell differentiation and angiogenesis were among biological processes indicated by this system. In conclusion, microarray technique is a useful tool to study gene expression in the secretory phase-endometrium of RM patients. The differences in endometrial gene expressions between healthy and RM subjects contribute to an increase in our knowledge on molecular mechanisms of RM development and may improve the outcome of pregnancies in high-risk women with RM.

Original languageEnglish
Pages (from-to)183-199
Number of pages17
JournalReproductive biology
Volume12
Issue number2
Publication statusPublished - 2012

Fingerprint

Habitual Abortion
abortion (animals)
endometrium
Endometrium
Genes
genes
cell adhesion
Fibroblast Growth Factor 9
transcription (genetics)
macrophage colony-stimulating factor
Biological Phenomena
Gene Expression
High-Risk Pregnancy
STAT3 Transcription Factor
gene expression
fibroblast growth factors
pregnancy outcome
Macrophage Colony-Stimulating Factor
Secretory Pathway
integrins

Keywords

  • Gene expression
  • Genomics
  • Microarray
  • Recurrent miscarriage

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Developmental Biology
  • Endocrinology

Cite this

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title = "Microarray profiling of secretoryphase endometrium from patients with recurrent miscarriage",
abstract = "The aim of the present study was to identify differentially expressed genes and their related biological pathways in the secretory phase endometrium from patients with recurrent miscarriage (RM) and fertile subjects. Endometrial samples from RM and fertile patients were analyzed using the Affymetrix GeneChip{\circledR} ST Array. The bioinformatic analysis using the Partek Genomic Suite revealed 346 genes that were differentially expressed (175 up-regulated and 171 down-regulated) in the endometrium of RM patients compared to the fertile subjects (fold change ≥1.5, p<0.005). Validation step using quantitative real-time polymerase chain reaction (qPCR) confirmed a similar expression pattern of four exemplary genes: one up-regulated gene (fibroblast growth factor 9, FGF9) and three down-regulated genes: integrin β3 (ITGB3), colony stimulating factor 1 (CSF1) and matrix-metalloproteinases 19 (MMP19). The Gene Set Enrichment Analysis (GSEA) and the Pathway Studio Software have found 101 signaling pathways (p<0.05) associated with the affected genes including the FGFR 3/signal transducer and activator of transcription (STAT) pathway and the CSF1R/STAT pathway. Cell adhesion, cell differentiation and angiogenesis were among biological processes indicated by this system. In conclusion, microarray technique is a useful tool to study gene expression in the secretory phase-endometrium of RM patients. The differences in endometrial gene expressions between healthy and RM subjects contribute to an increase in our knowledge on molecular mechanisms of RM development and may improve the outcome of pregnancies in high-risk women with RM.",
keywords = "Gene expression, Genomics, Microarray, Recurrent miscarriage",
author = "Rosfayati Othman and Omar, {Mohd Hashim} and Lim, {Pei Shan} and Shafiee, {Mohamad Nasir} and {A. Jamal}, {A. Rahman} and {Mohd Mokhtar}, Norfilza",
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T1 - Microarray profiling of secretoryphase endometrium from patients with recurrent miscarriage

AU - Othman, Rosfayati

AU - Omar, Mohd Hashim

AU - Lim, Pei Shan

AU - Shafiee, Mohamad Nasir

AU - A. Jamal, A. Rahman

AU - Mohd Mokhtar, Norfilza

PY - 2012

Y1 - 2012

N2 - The aim of the present study was to identify differentially expressed genes and their related biological pathways in the secretory phase endometrium from patients with recurrent miscarriage (RM) and fertile subjects. Endometrial samples from RM and fertile patients were analyzed using the Affymetrix GeneChip® ST Array. The bioinformatic analysis using the Partek Genomic Suite revealed 346 genes that were differentially expressed (175 up-regulated and 171 down-regulated) in the endometrium of RM patients compared to the fertile subjects (fold change ≥1.5, p<0.005). Validation step using quantitative real-time polymerase chain reaction (qPCR) confirmed a similar expression pattern of four exemplary genes: one up-regulated gene (fibroblast growth factor 9, FGF9) and three down-regulated genes: integrin β3 (ITGB3), colony stimulating factor 1 (CSF1) and matrix-metalloproteinases 19 (MMP19). The Gene Set Enrichment Analysis (GSEA) and the Pathway Studio Software have found 101 signaling pathways (p<0.05) associated with the affected genes including the FGFR 3/signal transducer and activator of transcription (STAT) pathway and the CSF1R/STAT pathway. Cell adhesion, cell differentiation and angiogenesis were among biological processes indicated by this system. In conclusion, microarray technique is a useful tool to study gene expression in the secretory phase-endometrium of RM patients. The differences in endometrial gene expressions between healthy and RM subjects contribute to an increase in our knowledge on molecular mechanisms of RM development and may improve the outcome of pregnancies in high-risk women with RM.

AB - The aim of the present study was to identify differentially expressed genes and their related biological pathways in the secretory phase endometrium from patients with recurrent miscarriage (RM) and fertile subjects. Endometrial samples from RM and fertile patients were analyzed using the Affymetrix GeneChip® ST Array. The bioinformatic analysis using the Partek Genomic Suite revealed 346 genes that were differentially expressed (175 up-regulated and 171 down-regulated) in the endometrium of RM patients compared to the fertile subjects (fold change ≥1.5, p<0.005). Validation step using quantitative real-time polymerase chain reaction (qPCR) confirmed a similar expression pattern of four exemplary genes: one up-regulated gene (fibroblast growth factor 9, FGF9) and three down-regulated genes: integrin β3 (ITGB3), colony stimulating factor 1 (CSF1) and matrix-metalloproteinases 19 (MMP19). The Gene Set Enrichment Analysis (GSEA) and the Pathway Studio Software have found 101 signaling pathways (p<0.05) associated with the affected genes including the FGFR 3/signal transducer and activator of transcription (STAT) pathway and the CSF1R/STAT pathway. Cell adhesion, cell differentiation and angiogenesis were among biological processes indicated by this system. In conclusion, microarray technique is a useful tool to study gene expression in the secretory phase-endometrium of RM patients. The differences in endometrial gene expressions between healthy and RM subjects contribute to an increase in our knowledge on molecular mechanisms of RM development and may improve the outcome of pregnancies in high-risk women with RM.

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