Micro-computed tomography detection of gold nanoparticle-labelled mesenchymal stem cells in the rat subretinal layer

Pooi Ling Mok, Sue Ngein Leow, Avin Ee Hwan Koh, Hairul Harun Mohd Nizam, Suet Lee Shirley Ding, Chi Luu, Raduan Ruhaslizan, Hon Seng Wong, Wan Haslina Wan Abdul Halim, Min Hwei Ng, Ruszymah Idrus, Shiplu Roy Chowdhury, Mae-Lynn Catherine Bastion, Suresh Kumar Subbiah, Akon Higuchi, Abdullah A. Alarfaj, Kong Yong Then

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Mesenchymal stem cells are widely used in many pre-clinical and clinical settings. Despite advances in molecular technology; the migration and homing activities of these cells in in vivo systems are not well understood. Labelling mesenchymal stem cells with gold nanoparticles has no cytotoxic effect and may offer suitable indications for stem cell tracking. Here, we report a simple protocol to label mesenchymal stem cells using 80 nm gold nanoparticles. Once the cells and particles were incubated together for 24 h, the labelled products were injected into the rat subretinal layer. Micro-computed tomography was then conducted on the 15th and 30th day post-injection to track the movement of these cells, as visualized by an area of hyperdensity from the coronal section images of the rat head. In addition, we confirmed the cellular uptake of the gold nanoparticles by the mesenchymal stem cells using transmission electron microscopy. As opposed to other methods, the current protocol provides a simple, less labour-intensive and more efficient labelling mechanism for real-time cell tracking. Finally, we discuss the potential manipulations of gold nanoparticles in stem cells for cell replacement and cancer therapy in ocular disorders or diseases.

Original languageEnglish
Article number345
JournalInternational Journal of Molecular Sciences
Volume18
Issue number2
DOIs
Publication statusPublished - 8 Feb 2017

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stem cells
Stem cells
Mesenchymal Stromal Cells
Gold
Nanoparticles
rats
Tomography
Rats
tomography
Cell Tracking
gold
nanoparticles
cells
Stem Cells
Labeling
marking
Transmission Electron Microscopy
homing
Cell Movement
labor

Keywords

  • Gold nanoparticles
  • Mesenchymal stem cells
  • Micro-computed tomography
  • Rat subretinal layer
  • Stem cell tracking
  • Transmission electron microscopy

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Computer Science Applications
  • Spectroscopy
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

Micro-computed tomography detection of gold nanoparticle-labelled mesenchymal stem cells in the rat subretinal layer. / Mok, Pooi Ling; Leow, Sue Ngein; Koh, Avin Ee Hwan; Mohd Nizam, Hairul Harun; Ding, Suet Lee Shirley; Luu, Chi; Ruhaslizan, Raduan; Wong, Hon Seng; Wan Abdul Halim, Wan Haslina; Ng, Min Hwei; Idrus, Ruszymah; Chowdhury, Shiplu Roy; Bastion, Mae-Lynn Catherine; Subbiah, Suresh Kumar; Higuchi, Akon; Alarfaj, Abdullah A.; Then, Kong Yong.

In: International Journal of Molecular Sciences, Vol. 18, No. 2, 345, 08.02.2017.

Research output: Contribution to journalArticle

Mok, Pooi Ling ; Leow, Sue Ngein ; Koh, Avin Ee Hwan ; Mohd Nizam, Hairul Harun ; Ding, Suet Lee Shirley ; Luu, Chi ; Ruhaslizan, Raduan ; Wong, Hon Seng ; Wan Abdul Halim, Wan Haslina ; Ng, Min Hwei ; Idrus, Ruszymah ; Chowdhury, Shiplu Roy ; Bastion, Mae-Lynn Catherine ; Subbiah, Suresh Kumar ; Higuchi, Akon ; Alarfaj, Abdullah A. ; Then, Kong Yong. / Micro-computed tomography detection of gold nanoparticle-labelled mesenchymal stem cells in the rat subretinal layer. In: International Journal of Molecular Sciences. 2017 ; Vol. 18, No. 2.
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