Methoxylated flavones from Salvia Mirzayanii Rech. F. and esfand with immunosuppressive properties

Abdul Majid Ayatollahi, Mustafa Ghanadian, Rahman Att-Ur-Rahman, Osman Mesaik Mohammed Ahmed Hassan, Ahmad Shukralla Khalid, Fateme Adeli

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Aerial parts of Salvia Mirzayanii was extracted with methanol. Methanol extract was suspended in water and defatted with petroleum ether. The defatted part was then partitioned between ethyl acetate and water. The ethyl acetate partition was chromatographed on a silica gel column to afford several fractions. Lymphocyte proliferation inhibitory assay of the resulted fractions was compared in-vitro and the fraction with more immunosuppressive activity was subjected to more purification to yield three methoxylated flavones: 5,7-dihydroxy,6,4’– dimethoxyflavone(1), 5-hydroxy,6,7,3’,4’–tetramethoxyflavone(2) and 5,3’-dihydroxy,6,7,4’– trimethoxyflavone (3). Compounds 2 and 3 potently suppressed the proliferation of human blood lymphocytes with IC50 values of 1.3 ± 0.04 μg/mL and 1.3 ± 0.21 μg/mL in comparison with prednisolone as one of the lymphocyte suppressor drugs (IC50=1.45 ± 0.6 μg/mL). In phagocyte chemiluminescence assay, compounds 1 and 3 in peripheral mononuclear cells (PMNCs) exerted suppressive moderate activity against ROS with IC50 of 55.3 ± 0.4 and 36.2 ± 0.7 μg/mL, respectively, while compound 2 showed weak activity with IC50 values more than 100 μg/mL. In conclusion, compounds 2 and 3 have a similar suppressive effect more than compound 1 on PHA-activated lymphocyte proliferation, which might be because of their C-3’ oxidation pattern of ring B. It is indicated that the presence of 3’-OH or 3’-OMe in flavone ring B, caused more anti-proliferation activity than 3’-H. Oxidative burst assay showed more activity for compound 1 which is less methoxylated than others. It also showed more activity for compounds 3 than 2, which differ only in 3’-OH instead of 3’-OMe.

Original languageEnglish
Pages (from-to)955-960
Number of pages6
JournalIranian Journal of Pharmaceutical Research
Volume14
Issue number3
Publication statusPublished - 1 Mar 2015
Externally publishedYes

Fingerprint

Salvia
Flavones
Immunosuppressive Agents
Inhibitory Concentration 50
Lymphocytes
flavone
Methanol
Respiratory Burst
Water
Silica Gel
Phagocytes
Prednisolone
Luminescence
Pharmaceutical Preparations
ethyl acetate

Keywords

  • Immunosuppressive
  • Methoxylated flavones
  • Oxidative burst
  • Salvia Mirzayanii
  • T-cell proliferation

ASJC Scopus subject areas

  • Pharmacology (medical)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Methoxylated flavones from Salvia Mirzayanii Rech. F. and esfand with immunosuppressive properties. / Ayatollahi, Abdul Majid; Ghanadian, Mustafa; Att-Ur-Rahman, Rahman; Mohammed Ahmed Hassan, Osman Mesaik; Khalid, Ahmad Shukralla; Adeli, Fateme.

In: Iranian Journal of Pharmaceutical Research, Vol. 14, No. 3, 01.03.2015, p. 955-960.

Research output: Contribution to journalArticle

Ayatollahi, Abdul Majid ; Ghanadian, Mustafa ; Att-Ur-Rahman, Rahman ; Mohammed Ahmed Hassan, Osman Mesaik ; Khalid, Ahmad Shukralla ; Adeli, Fateme. / Methoxylated flavones from Salvia Mirzayanii Rech. F. and esfand with immunosuppressive properties. In: Iranian Journal of Pharmaceutical Research. 2015 ; Vol. 14, No. 3. pp. 955-960.
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