Metabolomics differentiation of oil palm (Elaeis guineensis Jacq.) spear leaf with contrasting susceptibility to Ganoderma boninense

Nurul Liyana Rozali, Mohd. Ambar Yarmo, Abu Seman Idris, Ahmad Kushairi, Umi Salamah Ramli

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Basal stem rot (BSR) disease caused by Ganoderma boninense is the most serious and destructive disease in oil palm, especially in Southeast Asia and required urgent control measures to combat the disease outbreak. Information of understanding metabolite response of oil palm to BSR is limited. Therefore, parental palms with contrasting susceptibility to G. boninense based on previous oil palm progenies testing using root inoculation technique to identify oil palm progenies partially tolerant and susceptible to G. boninense were examined by metabolomics approach using gas chromatography x gas chromatography-time-of-flight mass spectrometry (GC×GC-TOF-MS). Analysis of metabolomics data from GC×GC-TOF-MS was conducted by supervised multivariate analysis of partial least squares-discriminant analysis (PLS) and orthogonal partial least squares-discriminant analysis (OPLS-DA) that allowed cross-validation and response permutation test functions. As a result, seven potential metabolites that contribute to the contrasting susceptibility of oil palms to G. boninense were identified as mannose, xylose, glucopyranose, myo-inositol and hexadecanoic acid which were found high in partially tolerant oil palm whereas cadaverine and turanose were found high in susceptible oil palm as observed in fold changes of detected GC×GC-TOF-MS peaks. The results suggest that the employed strategy is a potential approach to profile and characterize leaf metabolome with contrasting susceptibility to G. boninense. This result provide baseline in future studies utilizing metabolomics in identifying potential biomarkers by screening larger population of truly resistant palms to G. boninense.

Original languageEnglish
Pages (from-to)45-52
Number of pages8
JournalPlant OMICS
Volume10
Issue number2
DOIs
Publication statusPublished - 1 Mar 2017

Fingerprint

Elaeis guineensis
metabolomics
gas chromatography
leaves
stem rot
mass spectrometry
discriminant analysis
least squares
metabolites
root inoculation
metabolome
progeny testing
cadaverine
Ganoderma boninense
myo-inositol
mannose
xylose
South East Asia
palmitic acid
multivariate analysis

Keywords

  • Basal stem rot
  • Ganoderma boninense
  • GC×GC-TOF-MS
  • Metabolomics
  • Multivariate analysis
  • Oil palm (Elaeis guineensis) spear leaf

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

Metabolomics differentiation of oil palm (Elaeis guineensis Jacq.) spear leaf with contrasting susceptibility to Ganoderma boninense. / Rozali, Nurul Liyana; Yarmo, Mohd. Ambar; Idris, Abu Seman; Kushairi, Ahmad; Ramli, Umi Salamah.

In: Plant OMICS, Vol. 10, No. 2, 01.03.2017, p. 45-52.

Research output: Contribution to journalArticle

Rozali, Nurul Liyana ; Yarmo, Mohd. Ambar ; Idris, Abu Seman ; Kushairi, Ahmad ; Ramli, Umi Salamah. / Metabolomics differentiation of oil palm (Elaeis guineensis Jacq.) spear leaf with contrasting susceptibility to Ganoderma boninense. In: Plant OMICS. 2017 ; Vol. 10, No. 2. pp. 45-52.
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