Metabolomics approach to investigate phytotoxic effects of Wedelia trilobata leaves, litter and soil

Kamalrul Azlan Azizan, Sarah Ibrahim, Nurul Haizun Abdul Ghani, Mohammad Firdaus Nawawi

Research output: Contribution to journalArticle

Abstract

Although release and accumulation of plant metabolites from plant into soil can influence allelopathy, little information is known about metabolite changes that occur in leaf, litter and soil. In this study, seed germination bioassay tests and metabolomics analysis were performed to investigate the phytotoxic effects and metabolic variations (measured as buckets) in the ethanolic extracts of leaf, leaf litter and soil of Wedelia trilobata. Increasing the ethanolic extracts concentration of all extracts significantly inhibited Lactuca sativa germination rate (GR), shoot height (SH) and root length (RL). Soil exerted the strongest inhibition but contained the lowest number of buckets relative to those of leaf and leaf litter extracts. An overlap overview on the metabolome revealed a poor bucket overlap and redundancy among the leaf, leaf litter and soil extracts. Canonical correspondence analysis concluded that the SH of L. sativa was more sensitive to leaf litter extract and the leaf extract exerted a strong influence on the GR and RL of L. sativa. Multivariate analysis suggested that the metabolome of the leaf, leaf litter and soil differ substantially. Finally, putative identification using MS/MS data demonstrated various plant metabolites with phytotoxic effects that can contribute to the allelopathy of W. trilobata.

Original languageEnglish
JournalPlant Biosystems
DOIs
Publication statusAccepted/In press - 1 Jan 2018

Fingerprint

Sphagneticola trilobata
metabolomics
leaf litter
plant litter
buckets
Lactuca sativa
extracts
metabolome
soil
allelopathy
metabolite
germination
metabolites
leaf extracts
shoot
leaves
shoots
correspondence analysis
multivariate analysis
effect

Keywords

  • allelopathy
  • Metabolomics
  • multivariate statistical analysis
  • time-of-flight mass spectrometry
  • Wedelia trilobata

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Plant Science

Cite this

Metabolomics approach to investigate phytotoxic effects of Wedelia trilobata leaves, litter and soil. / Azizan, Kamalrul Azlan; Ibrahim, Sarah; Abdul Ghani, Nurul Haizun; Nawawi, Mohammad Firdaus.

In: Plant Biosystems, 01.01.2018.

Research output: Contribution to journalArticle

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