Metabolomics analysis of mangosteen (Garcinia mangostana Linn.) fruit pericarp using different extraction methods and GC-MS

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Garcinia mangostana Linn. (mangosteen) reportedly contains several bioactive metabolites which have been used in various traditional disease treatment, nutritional supplement and skincare products. However, reports detailing its characterization and metabolite content are still scarce. Most of the earlier reports only focused on the extraction of specific metabolites such as xanthones and anthocyanins, rather than a comprehensive metabolome profile. In this study, global metabolomics approach using gas chromatography-mass spectrometry (GC-MS) analysis was conducted to profile the metabolite content of mangosteen fruit pericarp harvested at the final stage of ripening (dark purple stage). Five different extraction methods, which vary in their solvent mixtures, solvent ratios, with or without sonication were compared, aimed to maximize metabolite detection from the mangosteen tissue. The results showed that the method using a combined solvent mixture of methanol/chloroform/water (3:1:1 v/v ratio) with sonication has consistent reproducibility amongst biological replicates and successfully yielded the highest number of metabolites compared to the other methods. By combining the results of different extraction methods, we tentatively identified a total of 73 metabolites comprising of sugars (49.32%), alcohols (9.59%), sugar acids (8.22%), organic acids (6.85%), phenolic acids (5.48%), aromatic compounds (2.74%) and aldehyde (1.37%). This finding provides an overview of the metabolite content of mangosteen pericarp and comprehensive assessment of extraction methods for an untargeted metabolomics approach of this beneficial fruit.

Original languageEnglish
Pages (from-to)89-97
Number of pages9
JournalPlant OMICS
Volume11
Issue number2
DOIs
Publication statusPublished - 1 May 2018

Fingerprint

Garcinia mangostana
mangosteens
metabolomics
pericarp
metabolites
fruits
methodology
sugar alcohols
xanthones
metabolome
gas chromatography-mass spectrometry
sugar acids
phenolic acids
chloroform
reproducibility
aldehydes
organic acids and salts
aromatic compounds
dietary supplements
anthocyanins

Keywords

  • Fruit
  • Garcinia mangostana Linn.
  • GC-MS
  • Metabolite extraction
  • Metabolomics
  • Sample preparation

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

@article{f5add7e106174d6d8453a6f4e075af63,
title = "Metabolomics analysis of mangosteen (Garcinia mangostana Linn.) fruit pericarp using different extraction methods and GC-MS",
abstract = "Garcinia mangostana Linn. (mangosteen) reportedly contains several bioactive metabolites which have been used in various traditional disease treatment, nutritional supplement and skincare products. However, reports detailing its characterization and metabolite content are still scarce. Most of the earlier reports only focused on the extraction of specific metabolites such as xanthones and anthocyanins, rather than a comprehensive metabolome profile. In this study, global metabolomics approach using gas chromatography-mass spectrometry (GC-MS) analysis was conducted to profile the metabolite content of mangosteen fruit pericarp harvested at the final stage of ripening (dark purple stage). Five different extraction methods, which vary in their solvent mixtures, solvent ratios, with or without sonication were compared, aimed to maximize metabolite detection from the mangosteen tissue. The results showed that the method using a combined solvent mixture of methanol/chloroform/water (3:1:1 v/v ratio) with sonication has consistent reproducibility amongst biological replicates and successfully yielded the highest number of metabolites compared to the other methods. By combining the results of different extraction methods, we tentatively identified a total of 73 metabolites comprising of sugars (49.32{\%}), alcohols (9.59{\%}), sugar acids (8.22{\%}), organic acids (6.85{\%}), phenolic acids (5.48{\%}), aromatic compounds (2.74{\%}) and aldehyde (1.37{\%}). This finding provides an overview of the metabolite content of mangosteen pericarp and comprehensive assessment of extraction methods for an untargeted metabolomics approach of this beneficial fruit.",
keywords = "Fruit, Garcinia mangostana Linn., GC-MS, Metabolite extraction, Metabolomics, Sample preparation",
author = "Mamat, {Siti Farah} and Azizan, {Kamalrul Azlan} and Baharum, {Syarul Nataqain} and {Mohd. Noor}, Normah and {Wan Kamaruddin}, {Wan Mohd Aizat}",
year = "2018",
month = "5",
day = "1",
doi = "10.21475/poj.11.02.18.pne1191",
language = "English",
volume = "11",
pages = "89--97",
journal = "Plant OMICS",
issn = "1836-0661",
publisher = "Southern Cross Publishing and Printing Pty Ltd",
number = "2",

}

TY - JOUR

T1 - Metabolomics analysis of mangosteen (Garcinia mangostana Linn.) fruit pericarp using different extraction methods and GC-MS

AU - Mamat, Siti Farah

AU - Azizan, Kamalrul Azlan

AU - Baharum, Syarul Nataqain

AU - Mohd. Noor, Normah

AU - Wan Kamaruddin, Wan Mohd Aizat

PY - 2018/5/1

Y1 - 2018/5/1

N2 - Garcinia mangostana Linn. (mangosteen) reportedly contains several bioactive metabolites which have been used in various traditional disease treatment, nutritional supplement and skincare products. However, reports detailing its characterization and metabolite content are still scarce. Most of the earlier reports only focused on the extraction of specific metabolites such as xanthones and anthocyanins, rather than a comprehensive metabolome profile. In this study, global metabolomics approach using gas chromatography-mass spectrometry (GC-MS) analysis was conducted to profile the metabolite content of mangosteen fruit pericarp harvested at the final stage of ripening (dark purple stage). Five different extraction methods, which vary in their solvent mixtures, solvent ratios, with or without sonication were compared, aimed to maximize metabolite detection from the mangosteen tissue. The results showed that the method using a combined solvent mixture of methanol/chloroform/water (3:1:1 v/v ratio) with sonication has consistent reproducibility amongst biological replicates and successfully yielded the highest number of metabolites compared to the other methods. By combining the results of different extraction methods, we tentatively identified a total of 73 metabolites comprising of sugars (49.32%), alcohols (9.59%), sugar acids (8.22%), organic acids (6.85%), phenolic acids (5.48%), aromatic compounds (2.74%) and aldehyde (1.37%). This finding provides an overview of the metabolite content of mangosteen pericarp and comprehensive assessment of extraction methods for an untargeted metabolomics approach of this beneficial fruit.

AB - Garcinia mangostana Linn. (mangosteen) reportedly contains several bioactive metabolites which have been used in various traditional disease treatment, nutritional supplement and skincare products. However, reports detailing its characterization and metabolite content are still scarce. Most of the earlier reports only focused on the extraction of specific metabolites such as xanthones and anthocyanins, rather than a comprehensive metabolome profile. In this study, global metabolomics approach using gas chromatography-mass spectrometry (GC-MS) analysis was conducted to profile the metabolite content of mangosteen fruit pericarp harvested at the final stage of ripening (dark purple stage). Five different extraction methods, which vary in their solvent mixtures, solvent ratios, with or without sonication were compared, aimed to maximize metabolite detection from the mangosteen tissue. The results showed that the method using a combined solvent mixture of methanol/chloroform/water (3:1:1 v/v ratio) with sonication has consistent reproducibility amongst biological replicates and successfully yielded the highest number of metabolites compared to the other methods. By combining the results of different extraction methods, we tentatively identified a total of 73 metabolites comprising of sugars (49.32%), alcohols (9.59%), sugar acids (8.22%), organic acids (6.85%), phenolic acids (5.48%), aromatic compounds (2.74%) and aldehyde (1.37%). This finding provides an overview of the metabolite content of mangosteen pericarp and comprehensive assessment of extraction methods for an untargeted metabolomics approach of this beneficial fruit.

KW - Fruit

KW - Garcinia mangostana Linn.

KW - GC-MS

KW - Metabolite extraction

KW - Metabolomics

KW - Sample preparation

UR - http://www.scopus.com/inward/record.url?scp=85056797173&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85056797173&partnerID=8YFLogxK

U2 - 10.21475/poj.11.02.18.pne1191

DO - 10.21475/poj.11.02.18.pne1191

M3 - Article

VL - 11

SP - 89

EP - 97

JO - Plant OMICS

JF - Plant OMICS

SN - 1836-0661

IS - 2

ER -