Metabolomic investigation of Elizabethkingia meningoseptica response challenge with Excoecaria agallocha leaf extract

A. A. Laith, Mazlan Abd. Ghaffar, T. S. Tengku Muhammad, L. K. Teh, M. Z. Salleh, M. Najiah

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Disease causes alterations in metabolism that may possibly be acknowledged as metabolic signatures and used to distinguish healthy and disease conditions.The present study was conducted for analytical platform that allows comprehensive analysis of microbial metabolomics. Different metabolites have been evaluated in vivo to characterize Elizabethkingia meningoseptica response on effects of methanol leaf extracts of Excoecaria agallocha using Liquid chromatography/mass spectrometry (LC/MS), utilizing a time-of-flight (TOF) mass analyzer. In vitro screening at different concentrations of E. agallochamethanol leaf extracts showed alteration in the shape of E.meningoseptica using scanning electron microscope (SEM). The profile of metabolites in vivo experiment showed 82 compounds that were initially identified.Only 75 compounds satisfy the P-Corrcut-off point of less than 0.05 and at least 2-fols change. In bacteria isolated from skin and kidney, initially a total of 240 metabolites were identified and only 79 metabolites satisfied the P-Corrcut-off point of less than 0.01 and at least 2-fold change. The result from scanning electron microscope (SEM) showed E.meningoseptica destroyed at concentration of 12.5 mg/mlof methanol leaf extracts of E. agallocha. To conclude, E. agallocha are diverse, produce compounds with antimicrobial activity and are a suitable sourcefor antimicrobial natural products.

Original languageEnglish
Pages (from-to)183-194
Number of pages12
JournalJournal of Pure and Applied Microbiology
Volume9
Issue numberSpecial Edition 1
Publication statusPublished - 1 Jan 2015
Externally publishedYes

Fingerprint

Euphorbiaceae
Metabolomics
Methanol
Electrons
Biological Products
Liquid Chromatography
Mass Spectrometry
Bacteria
Kidney
Skin
In Vitro Techniques

Keywords

  • Elizabethkingia meningoseptica
  • Excoecaria agallocha
  • LC/MS Q-TOF
  • Metabolites
  • SEM

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

Laith, A. A., Abd. Ghaffar, M., Tengku Muhammad, T. S., Teh, L. K., Salleh, M. Z., & Najiah, M. (2015). Metabolomic investigation of Elizabethkingia meningoseptica response challenge with Excoecaria agallocha leaf extract. Journal of Pure and Applied Microbiology, 9(Special Edition 1), 183-194.

Metabolomic investigation of Elizabethkingia meningoseptica response challenge with Excoecaria agallocha leaf extract. / Laith, A. A.; Abd. Ghaffar, Mazlan; Tengku Muhammad, T. S.; Teh, L. K.; Salleh, M. Z.; Najiah, M.

In: Journal of Pure and Applied Microbiology, Vol. 9, No. Special Edition 1, 01.01.2015, p. 183-194.

Research output: Contribution to journalArticle

Laith, AA, Abd. Ghaffar, M, Tengku Muhammad, TS, Teh, LK, Salleh, MZ & Najiah, M 2015, 'Metabolomic investigation of Elizabethkingia meningoseptica response challenge with Excoecaria agallocha leaf extract', Journal of Pure and Applied Microbiology, vol. 9, no. Special Edition 1, pp. 183-194.
Laith, A. A. ; Abd. Ghaffar, Mazlan ; Tengku Muhammad, T. S. ; Teh, L. K. ; Salleh, M. Z. ; Najiah, M. / Metabolomic investigation of Elizabethkingia meningoseptica response challenge with Excoecaria agallocha leaf extract. In: Journal of Pure and Applied Microbiology. 2015 ; Vol. 9, No. Special Edition 1. pp. 183-194.
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