Metabolite profiling of mangosteen seed germination highlights metabolic changes related to carbon utilization and seed protection

Research output: Contribution to journalArticle

Abstract

Mangosteen seed is categorized as recalcitrant, becoming inviable if exposed to desiccation and low temperature. However, the molecular mechanism of mangosteen seed germination has not been fully understood. This study profiled the metabolites that were present in germinating mangosteen seeds at different stages (zero, one, three, five, seven and nine days after sowing) using liquid chromatography-mass spectrometry (LC–MS) and gas chromatography-mass spectrometry (GC–MS). A total of 38 tentative metabolites were profiled and classified into amino acid, organic acid, sugar, polyol, alkaloid and others. Metabolites identified implied a timed regulation for germination. Sugars as well as amino acids exhibited a declining trend throughout the germination period as they are likely to be utilized for development and growth of seedling. Furthermore, secondary metabolites such as alkaloids, flavonoids, and xanthone presence displayed an increasing trend at the middle of germination period which may provide protection for mangosteen seed against herbivory and stress. In brief, carbon utilization and seed protection associated with the modulation of primary and secondary metabolites, respectively are important metabolic signatures of mangosteen seed germination.

LanguageEnglish
Pages226-234
Number of pages9
JournalScientia Horticulturae
Volume243
DOIs
Publication statusPublished - 3 Jan 2019

Fingerprint

metabolite
germination
seed
carbon
alkaloid
secondary metabolite
sugar
amino acid
mass spectrometry
desiccation
sowing
organic acid
growth and development
herbivory
liquid chromatography
gas chromatography
seedling
trend

Keywords

  • Garcinia mangostana L.
  • GC–MS
  • Germination
  • LC–MS
  • Metabolomics
  • Recalcitrant seed

ASJC Scopus subject areas

  • Horticulture

Cite this

@article{ca5bb6119d59487d909a576fc3a47592,
title = "Metabolite profiling of mangosteen seed germination highlights metabolic changes related to carbon utilization and seed protection",
abstract = "Mangosteen seed is categorized as recalcitrant, becoming inviable if exposed to desiccation and low temperature. However, the molecular mechanism of mangosteen seed germination has not been fully understood. This study profiled the metabolites that were present in germinating mangosteen seeds at different stages (zero, one, three, five, seven and nine days after sowing) using liquid chromatography-mass spectrometry (LC–MS) and gas chromatography-mass spectrometry (GC–MS). A total of 38 tentative metabolites were profiled and classified into amino acid, organic acid, sugar, polyol, alkaloid and others. Metabolites identified implied a timed regulation for germination. Sugars as well as amino acids exhibited a declining trend throughout the germination period as they are likely to be utilized for development and growth of seedling. Furthermore, secondary metabolites such as alkaloids, flavonoids, and xanthone presence displayed an increasing trend at the middle of germination period which may provide protection for mangosteen seed against herbivory and stress. In brief, carbon utilization and seed protection associated with the modulation of primary and secondary metabolites, respectively are important metabolic signatures of mangosteen seed germination.",
keywords = "Garcinia mangostana L., GC–MS, Germination, LC–MS, Metabolomics, Recalcitrant seed",
author = "Othman Mazlan and {Wan Kamaruddin}, {Wan Mohd Aizat} and {Aziz Zuddin}, {Nor Shahida} and Baharum, {Syarul Nataqain} and {Mohd. Noor}, Normah",
year = "2019",
month = "1",
day = "3",
doi = "10.1016/j.scienta.2018.08.022",
language = "English",
volume = "243",
pages = "226--234",
journal = "Scientia Horticulturae",
issn = "0304-4238",
publisher = "Elsevier",

}

TY - JOUR

T1 - Metabolite profiling of mangosteen seed germination highlights metabolic changes related to carbon utilization and seed protection

AU - Mazlan, Othman

AU - Wan Kamaruddin, Wan Mohd Aizat

AU - Aziz Zuddin, Nor Shahida

AU - Baharum, Syarul Nataqain

AU - Mohd. Noor, Normah

PY - 2019/1/3

Y1 - 2019/1/3

N2 - Mangosteen seed is categorized as recalcitrant, becoming inviable if exposed to desiccation and low temperature. However, the molecular mechanism of mangosteen seed germination has not been fully understood. This study profiled the metabolites that were present in germinating mangosteen seeds at different stages (zero, one, three, five, seven and nine days after sowing) using liquid chromatography-mass spectrometry (LC–MS) and gas chromatography-mass spectrometry (GC–MS). A total of 38 tentative metabolites were profiled and classified into amino acid, organic acid, sugar, polyol, alkaloid and others. Metabolites identified implied a timed regulation for germination. Sugars as well as amino acids exhibited a declining trend throughout the germination period as they are likely to be utilized for development and growth of seedling. Furthermore, secondary metabolites such as alkaloids, flavonoids, and xanthone presence displayed an increasing trend at the middle of germination period which may provide protection for mangosteen seed against herbivory and stress. In brief, carbon utilization and seed protection associated with the modulation of primary and secondary metabolites, respectively are important metabolic signatures of mangosteen seed germination.

AB - Mangosteen seed is categorized as recalcitrant, becoming inviable if exposed to desiccation and low temperature. However, the molecular mechanism of mangosteen seed germination has not been fully understood. This study profiled the metabolites that were present in germinating mangosteen seeds at different stages (zero, one, three, five, seven and nine days after sowing) using liquid chromatography-mass spectrometry (LC–MS) and gas chromatography-mass spectrometry (GC–MS). A total of 38 tentative metabolites were profiled and classified into amino acid, organic acid, sugar, polyol, alkaloid and others. Metabolites identified implied a timed regulation for germination. Sugars as well as amino acids exhibited a declining trend throughout the germination period as they are likely to be utilized for development and growth of seedling. Furthermore, secondary metabolites such as alkaloids, flavonoids, and xanthone presence displayed an increasing trend at the middle of germination period which may provide protection for mangosteen seed against herbivory and stress. In brief, carbon utilization and seed protection associated with the modulation of primary and secondary metabolites, respectively are important metabolic signatures of mangosteen seed germination.

KW - Garcinia mangostana L.

KW - GC–MS

KW - Germination

KW - LC–MS

KW - Metabolomics

KW - Recalcitrant seed

UR - http://www.scopus.com/inward/record.url?scp=85052055341&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85052055341&partnerID=8YFLogxK

U2 - 10.1016/j.scienta.2018.08.022

DO - 10.1016/j.scienta.2018.08.022

M3 - Article

VL - 243

SP - 226

EP - 234

JO - Scientia Horticulturae

T2 - Scientia Horticulturae

JF - Scientia Horticulturae

SN - 0304-4238

ER -