Metabolite profiles of callus and cell suspension cultures of mangosteen

Siti Zalikha Mohd Ridzuan Jamil, Emelda Rosseleena Rohani, Syarul Nataqain Baharum, Normah Mohd. Noor

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Callus was induced from mangosteen (Garcinia mangostana L.) young purple–red leaves on Murashige and Skoog basal medium with various combinations of plant growth regulators. Murashige and Skoog medium with 4.44 µM 6-benzylaminopurine and 4.52 µM 2,4-dichlorophenoxyacetic acid was the best for friable callus induction. This friable callus was used for the initiation of cell suspension culture. The effects of different combinations of 6-benzylaminopurine and 2,4-dichlorophenoxyacetic acid, carbon sources and inoculum sizes were tested. It was found that combination of 2.22 µM 6-benzylaminopurine + 2.26 µM 2,4-dichlorophenoxyacetic acid, glucose (30 g/l) and 1.5 g/50 ml inoculum size was the best for cell growth. Callus and cell suspension cultures were then treated either with 100 µM methyl jasmonate as an elicitor for 5 days, or 0.5 g/l casein hydrolysate as an organic supplement for 7 days. Metabolites were then extracted and profiled using liquid chromatography-time of flight mass spectrometry. Multivariate discriminant analyses revealed significant metabolite differences (P ≤ 0.05) for callus and suspension cells treated either with methyl jasmonate or casein hydrolysate. Based on MS/MS data, methyl jasmonate stimulated the production of an alkaloid (thalsimine) and fatty acid (phosphatidyl ethanolamine) in suspension cells while in callus, an alkaloid (thiacremonone) and glucosinolate (7-methylthioheptanaldoxime) was produced. Meanwhile casein hydrolysate stimulated the production of alkaloids such as 3ß,6ß-dihydroxynortropane and cis-hinokiresinol and triterpenoids such as schidigerasaponin and talinumoside in suspension cells. This study provides evidence on the potential of secondary metabolite production from in vitro culture of mangosteen.

Original languageEnglish
Article number322
Journal3 Biotech
Volume8
Issue number8
DOIs
Publication statusPublished - 1 Aug 2018

Fingerprint

Garcinia mangostana
mangosteens
N-benzyladenine
alkaloid
2,4 dichlorophenoxyacetic acid
Bony Callus
cell suspension culture
metabolite
Suspensions
callus
Cell Culture Techniques
metabolites
casein hydrolysates
2,4-Dichlorophenoxyacetic Acid
methyl jasmonate
Alkaloids
benzyladenine
2,4-D
alkaloids
growth regulator

Keywords

  • Callus induction
  • Casein hydrolysate
  • Cell suspension culture
  • Liquid chromatography–time of flight–mass spectrometry
  • Methyl jasmonate

ASJC Scopus subject areas

  • Biotechnology
  • Environmental Science (miscellaneous)
  • Agricultural and Biological Sciences (miscellaneous)

Cite this

Metabolite profiles of callus and cell suspension cultures of mangosteen. / Jamil, Siti Zalikha Mohd Ridzuan; Rohani, Emelda Rosseleena; Baharum, Syarul Nataqain; Mohd. Noor, Normah.

In: 3 Biotech, Vol. 8, No. 8, 322, 01.08.2018.

Research output: Contribution to journalArticle

Jamil, Siti Zalikha Mohd Ridzuan ; Rohani, Emelda Rosseleena ; Baharum, Syarul Nataqain ; Mohd. Noor, Normah. / Metabolite profiles of callus and cell suspension cultures of mangosteen. In: 3 Biotech. 2018 ; Vol. 8, No. 8.
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