Metabolic routes affecting rubber biosynthesis in Hevea brasiliensis latex

Keng See Chow, Mohd Noor Mat-Isa, Azlina Bahari, Ahmad Kamal Ghazali, Halimah Alias, Zainorlina Mohd.-Zainuddin, Chee Choong Hoh, Kiew Lian Wan

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

The cytosolic mevalonate (MVA) pathway in Hevea brasiliensis latex is the conventionally accepted pathway which provides isopentenyl diphosphate (IPP) for cis-polyisoprene (rubber) biosynthesis. However, the plastidic 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway may be an alternative source of IPP since its more recent discovery in plants. Quantitative RT-PCR (qRT-PCR) expression profiles of genes from both pathways in latex showed that subcellular compartmentalization of IPP for cis-polyisoprene synthesis is related to the degree of plastidic carotenoid synthesis. From this, the occurrence of two schemes of IPP partitioning and utilization within one species is proposed whereby the supply of IPP for cis-polyisoprene from the MEP pathway is related to carotenoid production in latex. Subsequently, a set of latex unique gene transcripts was sequenced and assembled and they were then mapped to IPP-requiring pathways. Up to eight such pathways, including cis-polyisoprene biosynthesis, were identified. Our findings on pre-and post-IPP metabolic routes form an important aspect of a pathway knowledge-driven approach to enhancing cis-polyisoprene biosynthesis in transgenic rubber trees.

Original languageEnglish
Pages (from-to)1863-1871
Number of pages9
JournalJournal of Experimental Botany
Volume63
Issue number5
DOIs
Publication statusPublished - Mar 2012

Fingerprint

Hevea
Hevea brasiliensis
Rubber
Latex
latex
rubber
biosynthesis
erythritol
carotenoids
phosphates
Carotenoids
synthesis
genes
reverse transcriptase polymerase chain reaction
genetically modified organisms
Mevalonic Acid
isopentenyl pyrophosphate
Transcriptome
Polymerase Chain Reaction

Keywords

  • cis-polyisoprene
  • gene expression
  • Hevea brasiliensis
  • isopentenyl diphosphate
  • isoprenoids
  • latex pathways
  • rubber biosynthesis
  • transcriptome

ASJC Scopus subject areas

  • Plant Science
  • Physiology

Cite this

Chow, K. S., Mat-Isa, M. N., Bahari, A., Ghazali, A. K., Alias, H., Mohd.-Zainuddin, Z., ... Wan, K. L. (2012). Metabolic routes affecting rubber biosynthesis in Hevea brasiliensis latex. Journal of Experimental Botany, 63(5), 1863-1871. https://doi.org/10.1093/jxb/err363

Metabolic routes affecting rubber biosynthesis in Hevea brasiliensis latex. / Chow, Keng See; Mat-Isa, Mohd Noor; Bahari, Azlina; Ghazali, Ahmad Kamal; Alias, Halimah; Mohd.-Zainuddin, Zainorlina; Hoh, Chee Choong; Wan, Kiew Lian.

In: Journal of Experimental Botany, Vol. 63, No. 5, 03.2012, p. 1863-1871.

Research output: Contribution to journalArticle

Chow, KS, Mat-Isa, MN, Bahari, A, Ghazali, AK, Alias, H, Mohd.-Zainuddin, Z, Hoh, CC & Wan, KL 2012, 'Metabolic routes affecting rubber biosynthesis in Hevea brasiliensis latex', Journal of Experimental Botany, vol. 63, no. 5, pp. 1863-1871. https://doi.org/10.1093/jxb/err363
Chow KS, Mat-Isa MN, Bahari A, Ghazali AK, Alias H, Mohd.-Zainuddin Z et al. Metabolic routes affecting rubber biosynthesis in Hevea brasiliensis latex. Journal of Experimental Botany. 2012 Mar;63(5):1863-1871. https://doi.org/10.1093/jxb/err363
Chow, Keng See ; Mat-Isa, Mohd Noor ; Bahari, Azlina ; Ghazali, Ahmad Kamal ; Alias, Halimah ; Mohd.-Zainuddin, Zainorlina ; Hoh, Chee Choong ; Wan, Kiew Lian. / Metabolic routes affecting rubber biosynthesis in Hevea brasiliensis latex. In: Journal of Experimental Botany. 2012 ; Vol. 63, No. 5. pp. 1863-1871.
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