Metabolic fingerprinting of the Lycopodiales species for chemotaxonomy and quality control

Chee Yan Choo, Norshahidah Sahidan, A. Latiff

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Herbs are used either singly or in combination as an alternative to drugs for the treatment of a variety of diseases. The advancement of technology has enabled more stringent quality control of herbs. For example, (-) Huperzine A is an alkaloid originally isolated from Huperzia serrata and has been found to be a potent, reversible and selective acetycholinesterase inhibitor. (-) Huperzine A is found in other Lycopodiales species. The aim of this study is to develop chemical profiles of Lycopodiales species with the hplc-pda system. Chemical profiles are analyzed with the chemometrics software. Species from the Lycopodiales order were collected throughout Peninsular Malaysia. Methanolic extracts of dried club mosses were dissolved in methanol and subjected to hplc analysis. The chemical profile was acquired with a hplc-pda connected to a 250 x 4.6mm ODS-3, 3 urn column maintained at 35 °C. A gradient system was used for the separation of (-) huperzine A. Data were collected from 200-500nm. The chemical profiles were exported to the UnScramblerX software. Using the unsupervised principal component analysis, the species were clustered into two families, namely the Huperziaceae and Lycopodiaceae families. The species can be chemically discriminated using the principal component analysis. This methodology is useful for both the chemotaxonomic discrimination of species from the Lycopodiales order and quality control of plant material.

Original languageEnglish
Title of host publicationACS Symposium Series
PublisherAmerican Chemical Society
Pages353-360
Number of pages8
Volume1185
ISBN (Print)9780841229761
Publication statusPublished - 2014
Externally publishedYes

Publication series

NameACS Symposium Series
Volume1185
ISSN (Print)00976156
ISSN (Electronic)19475918

Fingerprint

Principal component analysis
Quality control
Alkaloids
Methanol
Pharmaceutical Preparations
huperzine A

Keywords

  • Chemotaxonomy
  • Lycopodiales
  • Metabolic fingerprint

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Choo, C. Y., Sahidan, N., & Latiff, A. (2014). Metabolic fingerprinting of the Lycopodiales species for chemotaxonomy and quality control. In ACS Symposium Series (Vol. 1185, pp. 353-360). (ACS Symposium Series; Vol. 1185). American Chemical Society.

Metabolic fingerprinting of the Lycopodiales species for chemotaxonomy and quality control. / Choo, Chee Yan; Sahidan, Norshahidah; Latiff, A.

ACS Symposium Series. Vol. 1185 American Chemical Society, 2014. p. 353-360 (ACS Symposium Series; Vol. 1185).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Choo, CY, Sahidan, N & Latiff, A 2014, Metabolic fingerprinting of the Lycopodiales species for chemotaxonomy and quality control. in ACS Symposium Series. vol. 1185, ACS Symposium Series, vol. 1185, American Chemical Society, pp. 353-360.
Choo CY, Sahidan N, Latiff A. Metabolic fingerprinting of the Lycopodiales species for chemotaxonomy and quality control. In ACS Symposium Series. Vol. 1185. American Chemical Society. 2014. p. 353-360. (ACS Symposium Series).
Choo, Chee Yan ; Sahidan, Norshahidah ; Latiff, A. / Metabolic fingerprinting of the Lycopodiales species for chemotaxonomy and quality control. ACS Symposium Series. Vol. 1185 American Chemical Society, 2014. pp. 353-360 (ACS Symposium Series).
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