Melatonin ameliorates the adverse effects of leptin on sperm

Fayez A. Almabhouh, Khairul Osman, Siti Fatimah Ibrahim, Sergey Gupalo, Justin Gnanou, Effendi Ibrahim, Harbindar Jeet Singh

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

This study examined the effects of melatonin on leptin-induced changes in sperm parameters in adult rats. Five groups of Sprague-Dawley rats were treated with either leptin or leptin and melatonin or melatonin for 6 weeks. Leptin was given daily via the intraperitoneal route (60 μg kg-1 body weight) and melatonin was given in drinking water (10 mg kg-1 or 20 mg kg-1 body weight per day). Upon completion, sperm count, sperm morphology, 8-hydroxy-2-deoxyguanosine, Comet assay, TUNEL assay, gene expression profiles of antioxidant enzymes, respiratory chain reaction enzymes, DNA damage, and apoptosis genes were estimated. Data were analyzed using ANOVA. Sperm count was significantly lower whereas the fraction of sperm with abnormal morphology, the level of 8-hydroxy-2-deoxyguanosine, and sperm DNA fragmentation were significantly higher in rats treated with leptin only. Microarray analysis revealed significant upregulation of apoptosis-inducing factor, histone acetyl transferase, respiratory chain reaction enzyme, cell necrosis and DNA repair genes, and downregulation of antioxidant enzyme genes in leptin-treated rats. Real-time polymerase chain reaction showed significant decreases in glutathione peroxidase 1 expression with increases in the expression of apoptosis-inducing factor and histone acetyl transferase in leptin-treated rats. There was no change in the gene expression of caspase-3 (CASP-3). In conclusion, the adverse effects of leptin on sperm can be prevented by concurrent melatonin administration.

Original languageEnglish
JournalAsian Journal of Andrology
Volume18
DOIs
Publication statusPublished - 1 Jan 2016

Fingerprint

Melatonin
Leptin
Spermatozoa
Apoptosis Inducing Factor
Sperm Count
Enzymes
Electron Transport
Transferases
Histones
Antioxidants
Body Weight
Genes
Comet Assay
In Situ Nick-End Labeling
DNA Fragmentation
Microarray Analysis
Transcriptome
Caspase 3
DNA Repair
Drinking Water

Keywords

  • 8-hydroxy-2-deoxyguanosine
  • DNA fragmentation
  • Leptin
  • Melatonin
  • Sperm

ASJC Scopus subject areas

  • Urology

Cite this

Melatonin ameliorates the adverse effects of leptin on sperm. / Almabhouh, Fayez A.; Osman, Khairul; Ibrahim, Siti Fatimah; Gupalo, Sergey; Gnanou, Justin; Ibrahim, Effendi; Singh, Harbindar Jeet.

In: Asian Journal of Andrology, Vol. 18, 01.01.2016.

Research output: Contribution to journalArticle

Almabhouh, Fayez A. ; Osman, Khairul ; Ibrahim, Siti Fatimah ; Gupalo, Sergey ; Gnanou, Justin ; Ibrahim, Effendi ; Singh, Harbindar Jeet. / Melatonin ameliorates the adverse effects of leptin on sperm. In: Asian Journal of Andrology. 2016 ; Vol. 18.
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