Medium optimization for chitinase production from Trichoderma virens using central composite design

Azaliza Safarida Wasli, Madihah Md Salleh, Suraini Abd-Aziz, Osman Hassan, Nor Muhammad Mahadi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Medium development for chitinase production by Trichoderma virens was first carried out using conventional method of one-factor-at-a-time. The medium was further optimized using Central Composite Design in which response surface was generated later from the derived model. An experimental design of four variables including various initial pH values, chitin, ammonium sulphate, and methanol concentrations were created using Design Expert® Software, Version 6.0. The design consists of 30 experiments, which include 6 replicates at center points. The optimal value for each variable are 3.0 g/L, chitin; 0.1 g/L, ammonium sulphate; 0.4% (v/v), methanol; and initial pH, 4.0 with predicted chitinase activity of 0.1495 U/mL. These predicted parameters were tested in the laboratory and the final chitinase activity obtained was 0.1471 U/mL, which is almost reaching the predicted value. The optimal medium design showed an improvement of chitinase activity of 80.9% compared to activity obtained from the original Absidia medium composition.

Original languageEnglish
Pages (from-to)781-787
Number of pages7
JournalBiotechnology and Bioprocess Engineering
Volume14
Issue number6
DOIs
Publication statusPublished - Dec 2009

Fingerprint

Chitinases
Trichoderma
Chitin
Composite materials
Ammonium Sulfate
Methanol
Absidia
Software Design
Design of experiments
Research Design
Chemical analysis
Experiments
Sulfates

Keywords

  • Central composite design
  • Chitinase
  • Experimental design
  • Optimisation
  • Response surface methodology
  • Trichodema virens

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Biomedical Engineering
  • Bioengineering

Cite this

Medium optimization for chitinase production from Trichoderma virens using central composite design. / Wasli, Azaliza Safarida; Salleh, Madihah Md; Abd-Aziz, Suraini; Hassan, Osman; Mahadi, Nor Muhammad.

In: Biotechnology and Bioprocess Engineering, Vol. 14, No. 6, 12.2009, p. 781-787.

Research output: Contribution to journalArticle

Wasli, Azaliza Safarida ; Salleh, Madihah Md ; Abd-Aziz, Suraini ; Hassan, Osman ; Mahadi, Nor Muhammad. / Medium optimization for chitinase production from Trichoderma virens using central composite design. In: Biotechnology and Bioprocess Engineering. 2009 ; Vol. 14, No. 6. pp. 781-787.
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