Mediatorless impedance studies with titanium dioxide conjugated gold nanoparticles for hydrogen peroxide detection

Nur Hamidah Abdul Halim, Yook Heng Lee, Radha Swathe Priya Malon Marugan, Uda Hashim

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

An impedimetric-based biosensor constructed using gold nanoparticles (AuNP) entrapped within titanium dioxide (TiO2) particles for hydrogen peroxide (H2O2) detection is the main feature of this research. The matrix of the biosensor employed the surface of TiO2, which was previously modified with an amine terminal group using 3-Aminopropyltriethoxysilane (APTS) at a low temperature to create a ready to immobilise surface for the biosensor application. Hemoglobin (Hb), which exhibits peroxidase-like activity, was used as the bioreceptor in the biosensor to detect H2O2 in solution. The analysis was carried out using an alternative impedance method, in which the biosensor exhibited a wide linear range response between 1 × 10-4 M and 1.5 × 10-2 M and a limit of detection (LOD) of 1 × 10-5 M without a redox mediator.

Original languageEnglish
Article number38
JournalBiosensors
Volume7
Issue number3
DOIs
Publication statusPublished - 18 Sep 2017

Fingerprint

Biosensing Techniques
Electric Impedance
Biosensors
Gold
Nanoparticles
Hydrogen Peroxide
Peroxidase
Oxidation-Reduction
Amines
Limit of Detection
Hemoglobins
titanium dioxide
Temperature
Research

Keywords

  • Aminated titanium dioxide
  • Direct electron transfer
  • Hemoglobin
  • Impedimetric biosensor
  • Mediatorless

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Mediatorless impedance studies with titanium dioxide conjugated gold nanoparticles for hydrogen peroxide detection. / Halim, Nur Hamidah Abdul; Lee, Yook Heng; Marugan, Radha Swathe Priya Malon; Hashim, Uda.

In: Biosensors, Vol. 7, No. 3, 38, 18.09.2017.

Research output: Contribution to journalArticle

Halim, Nur Hamidah Abdul ; Lee, Yook Heng ; Marugan, Radha Swathe Priya Malon ; Hashim, Uda. / Mediatorless impedance studies with titanium dioxide conjugated gold nanoparticles for hydrogen peroxide detection. In: Biosensors. 2017 ; Vol. 7, No. 3.
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