Media improvement for hydrogen production using C. acetobutylicum NCIMB 13357

Mohd. Sahaid Kalil, Hisham Salem Saleh Alshiyab, Wan Mohtar Wan Yusoff

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Problem statement: Some component of fermentation medium showed to reduce the bacterial production of hydrogen. Approach: Reinforced clostridium medium is a selected medium for Clostridium species. Reformulation this medium regarding hydrogen production may focus on such medium composition that enhance or reduce the bacterial productivity. The optimum pH and temperature for hydrogen production were at initial pH of 7.0 and 30°C. Results: The results show that both nitrogen source and its concentration affected biomass growth as well as H2 yield. Yeast extract at concentration of 13 gL-1 was the best organic nitrogen source and resulted in hydrogen yield (YP/S) of 308 mL g-1glucose utilized with biomass concentration of 1.1 gL-1, hydrogen yield per biomass (YP/X) of 280 mL g-1L-1, biomass per substrate utilized (YX/S) of 0.22 and produced hydrogen in gram per gram of glucose utilized (YH2/S) of 0.0275. C/N of 70 enhanced the YP/S from 308 mL g-1to 350 mL g-1 glucose utilized with biomass concentration of 1.22 gL-1, YP/X of 287 mL g-1L-1, YX/S of 0.244 and (YH2/S) of 0.03125. In the absence of sodium chloride and sodium acetate further enhanced YP/S from 350 mL g-1glucose utilized to 391 mL g-1glucose utilized with maximum hydrogen productivity of 77.5 mL L-1 h-1, whereas RCM medium gave the highest hydrogen productivity of 63.5 mL L-1h-1. Results also show that Sodium Chloride and Sodium Acetate in the medium adversely affect growth. Removal of both components from the medium enhanced the biomass concentration from 1.22- 1.34 gL-1, YP/X of 254 mL g-1L-1, YX/S of 0.268 and (YH2/S) of 0.0349. Conclusion: The medium an improved containing (glucose 5 gL-1, Yeast extract gL-1, L-Cystine. HCl 1 gL-1 and Bacteriological agar 0.5 gL-1), was able to enhance the hydrogen productivity.

Original languageEnglish
Pages (from-to)1158-1168
Number of pages11
JournalAmerican Journal of Applied Sciences
Volume6
Issue number6
DOIs
Publication statusPublished - 2009

Fingerprint

Hydrogen
Biomass
Sodium Acetate
Clostridium
Glucose
Sodium Chloride
Nitrogen
Yeasts
Cystine
Growth
Fermentation
Agar
Temperature

Keywords

  • Biohydrogen
  • C.acetobutylicum NCIMB13357
  • Medium formulation
  • Salts

ASJC Scopus subject areas

  • General

Cite this

Media improvement for hydrogen production using C. acetobutylicum NCIMB 13357. / Kalil, Mohd. Sahaid; Alshiyab, Hisham Salem Saleh; Wan Yusoff, Wan Mohtar.

In: American Journal of Applied Sciences, Vol. 6, No. 6, 2009, p. 1158-1168.

Research output: Contribution to journalArticle

Kalil, Mohd. Sahaid ; Alshiyab, Hisham Salem Saleh ; Wan Yusoff, Wan Mohtar. / Media improvement for hydrogen production using C. acetobutylicum NCIMB 13357. In: American Journal of Applied Sciences. 2009 ; Vol. 6, No. 6. pp. 1158-1168.
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abstract = "Problem statement: Some component of fermentation medium showed to reduce the bacterial production of hydrogen. Approach: Reinforced clostridium medium is a selected medium for Clostridium species. Reformulation this medium regarding hydrogen production may focus on such medium composition that enhance or reduce the bacterial productivity. The optimum pH and temperature for hydrogen production were at initial pH of 7.0 and 30°C. Results: The results show that both nitrogen source and its concentration affected biomass growth as well as H2 yield. Yeast extract at concentration of 13 gL-1 was the best organic nitrogen source and resulted in hydrogen yield (YP/S) of 308 mL g-1glucose utilized with biomass concentration of 1.1 gL-1, hydrogen yield per biomass (YP/X) of 280 mL g-1L-1, biomass per substrate utilized (YX/S) of 0.22 and produced hydrogen in gram per gram of glucose utilized (YH2/S) of 0.0275. C/N of 70 enhanced the YP/S from 308 mL g-1to 350 mL g-1 glucose utilized with biomass concentration of 1.22 gL-1, YP/X of 287 mL g-1L-1, YX/S of 0.244 and (YH2/S) of 0.03125. In the absence of sodium chloride and sodium acetate further enhanced YP/S from 350 mL g-1glucose utilized to 391 mL g-1glucose utilized with maximum hydrogen productivity of 77.5 mL L-1 h-1, whereas RCM medium gave the highest hydrogen productivity of 63.5 mL L-1h-1. Results also show that Sodium Chloride and Sodium Acetate in the medium adversely affect growth. Removal of both components from the medium enhanced the biomass concentration from 1.22- 1.34 gL-1, YP/X of 254 mL g-1L-1, YX/S of 0.268 and (YH2/S) of 0.0349. Conclusion: The medium an improved containing (glucose 5 gL-1, Yeast extract gL-1, L-Cystine. HCl 1 gL-1 and Bacteriological agar 0.5 gL-1), was able to enhance the hydrogen productivity.",
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N2 - Problem statement: Some component of fermentation medium showed to reduce the bacterial production of hydrogen. Approach: Reinforced clostridium medium is a selected medium for Clostridium species. Reformulation this medium regarding hydrogen production may focus on such medium composition that enhance or reduce the bacterial productivity. The optimum pH and temperature for hydrogen production were at initial pH of 7.0 and 30°C. Results: The results show that both nitrogen source and its concentration affected biomass growth as well as H2 yield. Yeast extract at concentration of 13 gL-1 was the best organic nitrogen source and resulted in hydrogen yield (YP/S) of 308 mL g-1glucose utilized with biomass concentration of 1.1 gL-1, hydrogen yield per biomass (YP/X) of 280 mL g-1L-1, biomass per substrate utilized (YX/S) of 0.22 and produced hydrogen in gram per gram of glucose utilized (YH2/S) of 0.0275. C/N of 70 enhanced the YP/S from 308 mL g-1to 350 mL g-1 glucose utilized with biomass concentration of 1.22 gL-1, YP/X of 287 mL g-1L-1, YX/S of 0.244 and (YH2/S) of 0.03125. In the absence of sodium chloride and sodium acetate further enhanced YP/S from 350 mL g-1glucose utilized to 391 mL g-1glucose utilized with maximum hydrogen productivity of 77.5 mL L-1 h-1, whereas RCM medium gave the highest hydrogen productivity of 63.5 mL L-1h-1. Results also show that Sodium Chloride and Sodium Acetate in the medium adversely affect growth. Removal of both components from the medium enhanced the biomass concentration from 1.22- 1.34 gL-1, YP/X of 254 mL g-1L-1, YX/S of 0.268 and (YH2/S) of 0.0349. Conclusion: The medium an improved containing (glucose 5 gL-1, Yeast extract gL-1, L-Cystine. HCl 1 gL-1 and Bacteriological agar 0.5 gL-1), was able to enhance the hydrogen productivity.

AB - Problem statement: Some component of fermentation medium showed to reduce the bacterial production of hydrogen. Approach: Reinforced clostridium medium is a selected medium for Clostridium species. Reformulation this medium regarding hydrogen production may focus on such medium composition that enhance or reduce the bacterial productivity. The optimum pH and temperature for hydrogen production were at initial pH of 7.0 and 30°C. Results: The results show that both nitrogen source and its concentration affected biomass growth as well as H2 yield. Yeast extract at concentration of 13 gL-1 was the best organic nitrogen source and resulted in hydrogen yield (YP/S) of 308 mL g-1glucose utilized with biomass concentration of 1.1 gL-1, hydrogen yield per biomass (YP/X) of 280 mL g-1L-1, biomass per substrate utilized (YX/S) of 0.22 and produced hydrogen in gram per gram of glucose utilized (YH2/S) of 0.0275. C/N of 70 enhanced the YP/S from 308 mL g-1to 350 mL g-1 glucose utilized with biomass concentration of 1.22 gL-1, YP/X of 287 mL g-1L-1, YX/S of 0.244 and (YH2/S) of 0.03125. In the absence of sodium chloride and sodium acetate further enhanced YP/S from 350 mL g-1glucose utilized to 391 mL g-1glucose utilized with maximum hydrogen productivity of 77.5 mL L-1 h-1, whereas RCM medium gave the highest hydrogen productivity of 63.5 mL L-1h-1. Results also show that Sodium Chloride and Sodium Acetate in the medium adversely affect growth. Removal of both components from the medium enhanced the biomass concentration from 1.22- 1.34 gL-1, YP/X of 254 mL g-1L-1, YX/S of 0.268 and (YH2/S) of 0.0349. Conclusion: The medium an improved containing (glucose 5 gL-1, Yeast extract gL-1, L-Cystine. HCl 1 gL-1 and Bacteriological agar 0.5 gL-1), was able to enhance the hydrogen productivity.

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KW - Medium formulation

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