Mechanisms of the antihypertensive effects of Nigella sativa oil in L-NAME-induced hypertensive rats

Kamsiah Jaarin, Wai Dic Foong, Min Hui Yeoh, Zaman Yusoff Nik Kamarul, Qodriyah Mohd Saad, Azman Abdullah, Japar Sidik Fadhlullah Zuhair, Abdul Hamid Juliana, Kamisah Yusof

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

OBJECTIVES: This study was conducted to determine whether the blood pressure-lowering effect of Nigella sativa might be mediated by its effects on nitric oxide, angiotensin-converting enzyme, heme oxygenase and oxidative stress markers. METHODS: Twenty-four adult male Sprague-Dawley rats were divided equally into 4 groups. One group served as the control (group 1), whereas the other three groups (groups 2-4) were administered L-NAME (25 mg/kg, intraperitoneally). Groups 3 and 4 were given oral nicardipine daily at a dose of 3 mg/kg and Nigella sativa oil at a dose of 2.5 mg/kg for 8 weeks, respectively, concomitantly with L-NAME administration. RESULTS : Nigella sativa oil prevented the increase in systolic blood pressure in the L-NAME-treated rats. The blood pressure reduction was associated with a reduction in cardiac lipid peroxidation product, NADPH oxidase, angiotensin-converting enzyme activity and plasma nitric oxide, as well as with an increase in heme oxygenase-1 activity in the heart. The effects of Nigella sativa on blood pressure, lipid peroxidation product, nicotinamide adenine dinucleotide phosphate oxidase and angiotensin-converting enzyme were similar to those of nicardipine. In contrast, L-NAME had opposite effects on lipid peroxidation, angiotensin-converting enzyme and NO. CONCLUSION: The antihypertensive effect of Nigella sativa oil appears to be mediated by a reduction in cardiac oxidative stress and angiotensin-converting enzyme activity, an increase in cardiac heme oxygenase-1 activity and a prevention of plasma nitric oxide loss. Thus, Nigella sativa oil might be beneficial for controlling hypertension.

Original languageEnglish
Pages (from-to)751-757
Number of pages7
JournalClinics
Volume70
Issue number11
DOIs
Publication statusPublished - 1 Nov 2015

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NG-Nitroarginine Methyl Ester
Peptidyl-Dipeptidase A
Antihypertensive Agents
Blood Pressure
Nigella sativa
Lipid Peroxidation
Nicardipine
Nitric Oxide
Heme Oxygenase-1
Oxidative Stress
Heme Oxygenase (Decyclizing)
NADPH Oxidase
NADP
Sprague Dawley Rats
Oxidoreductases
caraway oil
Hypertension
Control Groups

Keywords

  • Angiotensin-converting Enzyme
  • Antihypertensive
  • Heme oxygenase
  • NADPH oxidase
  • Nigella sativa

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Mechanisms of the antihypertensive effects of Nigella sativa oil in L-NAME-induced hypertensive rats. / Jaarin, Kamsiah; Foong, Wai Dic; Yeoh, Min Hui; Kamarul, Zaman Yusoff Nik; Mohd Saad, Qodriyah; Abdullah, Azman; Zuhair, Japar Sidik Fadhlullah; Juliana, Abdul Hamid; Yusof, Kamisah.

In: Clinics, Vol. 70, No. 11, 01.11.2015, p. 751-757.

Research output: Contribution to journalArticle

Jaarin, Kamsiah ; Foong, Wai Dic ; Yeoh, Min Hui ; Kamarul, Zaman Yusoff Nik ; Mohd Saad, Qodriyah ; Abdullah, Azman ; Zuhair, Japar Sidik Fadhlullah ; Juliana, Abdul Hamid ; Yusof, Kamisah. / Mechanisms of the antihypertensive effects of Nigella sativa oil in L-NAME-induced hypertensive rats. In: Clinics. 2015 ; Vol. 70, No. 11. pp. 751-757.
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abstract = "OBJECTIVES: This study was conducted to determine whether the blood pressure-lowering effect of Nigella sativa might be mediated by its effects on nitric oxide, angiotensin-converting enzyme, heme oxygenase and oxidative stress markers. METHODS: Twenty-four adult male Sprague-Dawley rats were divided equally into 4 groups. One group served as the control (group 1), whereas the other three groups (groups 2-4) were administered L-NAME (25 mg/kg, intraperitoneally). Groups 3 and 4 were given oral nicardipine daily at a dose of 3 mg/kg and Nigella sativa oil at a dose of 2.5 mg/kg for 8 weeks, respectively, concomitantly with L-NAME administration. RESULTS : Nigella sativa oil prevented the increase in systolic blood pressure in the L-NAME-treated rats. The blood pressure reduction was associated with a reduction in cardiac lipid peroxidation product, NADPH oxidase, angiotensin-converting enzyme activity and plasma nitric oxide, as well as with an increase in heme oxygenase-1 activity in the heart. The effects of Nigella sativa on blood pressure, lipid peroxidation product, nicotinamide adenine dinucleotide phosphate oxidase and angiotensin-converting enzyme were similar to those of nicardipine. In contrast, L-NAME had opposite effects on lipid peroxidation, angiotensin-converting enzyme and NO. CONCLUSION: The antihypertensive effect of Nigella sativa oil appears to be mediated by a reduction in cardiac oxidative stress and angiotensin-converting enzyme activity, an increase in cardiac heme oxygenase-1 activity and a prevention of plasma nitric oxide loss. Thus, Nigella sativa oil might be beneficial for controlling hypertension.",
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AU - Jaarin, Kamsiah

AU - Foong, Wai Dic

AU - Yeoh, Min Hui

AU - Kamarul, Zaman Yusoff Nik

AU - Mohd Saad, Qodriyah

AU - Abdullah, Azman

AU - Zuhair, Japar Sidik Fadhlullah

AU - Juliana, Abdul Hamid

AU - Yusof, Kamisah

PY - 2015/11/1

Y1 - 2015/11/1

N2 - OBJECTIVES: This study was conducted to determine whether the blood pressure-lowering effect of Nigella sativa might be mediated by its effects on nitric oxide, angiotensin-converting enzyme, heme oxygenase and oxidative stress markers. METHODS: Twenty-four adult male Sprague-Dawley rats were divided equally into 4 groups. One group served as the control (group 1), whereas the other three groups (groups 2-4) were administered L-NAME (25 mg/kg, intraperitoneally). Groups 3 and 4 were given oral nicardipine daily at a dose of 3 mg/kg and Nigella sativa oil at a dose of 2.5 mg/kg for 8 weeks, respectively, concomitantly with L-NAME administration. RESULTS : Nigella sativa oil prevented the increase in systolic blood pressure in the L-NAME-treated rats. The blood pressure reduction was associated with a reduction in cardiac lipid peroxidation product, NADPH oxidase, angiotensin-converting enzyme activity and plasma nitric oxide, as well as with an increase in heme oxygenase-1 activity in the heart. The effects of Nigella sativa on blood pressure, lipid peroxidation product, nicotinamide adenine dinucleotide phosphate oxidase and angiotensin-converting enzyme were similar to those of nicardipine. In contrast, L-NAME had opposite effects on lipid peroxidation, angiotensin-converting enzyme and NO. CONCLUSION: The antihypertensive effect of Nigella sativa oil appears to be mediated by a reduction in cardiac oxidative stress and angiotensin-converting enzyme activity, an increase in cardiac heme oxygenase-1 activity and a prevention of plasma nitric oxide loss. Thus, Nigella sativa oil might be beneficial for controlling hypertension.

AB - OBJECTIVES: This study was conducted to determine whether the blood pressure-lowering effect of Nigella sativa might be mediated by its effects on nitric oxide, angiotensin-converting enzyme, heme oxygenase and oxidative stress markers. METHODS: Twenty-four adult male Sprague-Dawley rats were divided equally into 4 groups. One group served as the control (group 1), whereas the other three groups (groups 2-4) were administered L-NAME (25 mg/kg, intraperitoneally). Groups 3 and 4 were given oral nicardipine daily at a dose of 3 mg/kg and Nigella sativa oil at a dose of 2.5 mg/kg for 8 weeks, respectively, concomitantly with L-NAME administration. RESULTS : Nigella sativa oil prevented the increase in systolic blood pressure in the L-NAME-treated rats. The blood pressure reduction was associated with a reduction in cardiac lipid peroxidation product, NADPH oxidase, angiotensin-converting enzyme activity and plasma nitric oxide, as well as with an increase in heme oxygenase-1 activity in the heart. The effects of Nigella sativa on blood pressure, lipid peroxidation product, nicotinamide adenine dinucleotide phosphate oxidase and angiotensin-converting enzyme were similar to those of nicardipine. In contrast, L-NAME had opposite effects on lipid peroxidation, angiotensin-converting enzyme and NO. CONCLUSION: The antihypertensive effect of Nigella sativa oil appears to be mediated by a reduction in cardiac oxidative stress and angiotensin-converting enzyme activity, an increase in cardiac heme oxygenase-1 activity and a prevention of plasma nitric oxide loss. Thus, Nigella sativa oil might be beneficial for controlling hypertension.

KW - Angiotensin-converting Enzyme

KW - Antihypertensive

KW - Heme oxygenase

KW - NADPH oxidase

KW - Nigella sativa

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