Mechanism of chemoprevention against colon cancer cells using combined Gelam honey and Ginger extract via mTOR and Wnt/β-catenin pathways

Lee Heng Wee, Noor Azian Morad, Goon Jo Aan, Suzana Makpol, Wan Zurinah Wan Ngah, Yasmin Anum Mohd Yusof

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The PI3K-Akt-mTOR, Wnt/β-catenin and apoptosis signaling pathways have been shown to be involved in genesis of colorectal cancer (CRC). The aim of this study was to elucidate whether combination of Gelam honey and ginger might have chemopreventive properties in HT29 colon cancer cells by modulating the mTOR, Wnt/β-catenin and apoptosis signaling pathways. Treatment with Gelam honey and ginger reduced the viability of the HT29 cells dose dependently with IC50 values of 88 mg/ml and 2.15 mg/ml respectively, their while the combined treatment of 2 mg/ml of ginger with 31 mg/ml of Gelam honey inhibited growth of most HT29 cells. Gelam honey, ginger and combination induced apoptosis in a dose dependent manner with the combined treatment exhibiting the highest apoptosis rate. The combined treatment downregulated the gene expressions of Akt, mTOR, Raptor, Rictor, β-catenin, Gsk3β, Tcf4 and cyclin D1 while cytochrome C and caspase 3 genes were shown to be upregulated. In conclusion, the combination of Gelam honey and ginger may serve as a potential therapy in the treatment of colorectal cancer through inhibiton of mTOR, Wnt/β catenin signaling pathways and induction of apoptosis pathway.

Original languageEnglish
Pages (from-to)6549-6556
Number of pages8
JournalAsian Pacific Journal of Cancer Prevention
Volume16
Issue number15
DOIs
Publication statusPublished - 2015

Fingerprint

Ginger
Catenins
Wnt Signaling Pathway
Honey
Chemoprevention
Colonic Neoplasms
Apoptosis
HT29 Cells
Colorectal Neoplasms
Therapeutics
Raptors
Cyclin D1
Cytochromes
Phosphatidylinositol 3-Kinases
Caspase 3
Inhibitory Concentration 50
Down-Regulation
Gene Expression
Growth
Genes

Keywords

  • Apoptosis
  • Combination
  • HT29 colon cancer cells
  • mTOR
  • Wnt/β catenin

ASJC Scopus subject areas

  • Oncology
  • Cancer Research
  • Public Health, Environmental and Occupational Health
  • Epidemiology

Cite this

Mechanism of chemoprevention against colon cancer cells using combined Gelam honey and Ginger extract via mTOR and Wnt/β-catenin pathways. / Wee, Lee Heng; Morad, Noor Azian; Jo Aan, Goon; Makpol, Suzana; Wan Ngah, Wan Zurinah; Mohd Yusof, Yasmin Anum.

In: Asian Pacific Journal of Cancer Prevention, Vol. 16, No. 15, 2015, p. 6549-6556.

Research output: Contribution to journalArticle

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