Measurement of the relative proportion of symmetrical and asymmetrical chromosome-type interchanges induced by γ radiation in human-hamster hybrid cells

I. B. Bahari, J. S. Bedford, A. J. Giaccia, T. D. Stamato

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Using hamster-human hybrid cells and methods for differential staining of the hamster and human chromosomes, the relative proportion of induced chromosome-type symmetrical and asymmetrical interchange aberrations was measured after a 6.5-Gy dose of γ-radiation. The ratio of these aberration types was not significantly different from 1:1, i.e., the proportion of such interchanges that are symmetrical is very near 0.5, in agreement with the conclusion of others (J.A. Heddle, Genetics 52, 1329-1334, 1965; M. Holmberg and J. Jonasson, Hereditas 74, 57-68, 1973; J.R.K. Savage and D.G. Papworth, Mutat. Res. 95, 7-18, 1983). This proportion is important because virtually all estimates of radiation-induced chromosome-type exchange aberrations are based on measurement of the easily observed but unstable and lethal asymmetrical types, while some of the biological effects of concern from the point of view of oncogenesis and mutagenesis are thought to result from production of stable and nonlethal symmetrical types.

Original languageEnglish
Pages (from-to)105-107
Number of pages3
JournalRadiation Research
Volume123
Issue number1
DOIs
Publication statusPublished - 1990
Externally publishedYes

Fingerprint

hamsters
Hybrid Cells
chromosomes
Cricetinae
aberration
proportion
Chromosomes
Radiation
Radiation Dosage
Human Chromosomes
radiation
cells
Mutagenesis
Chromosome Aberrations
differential staining
mutagenesis
Carcinogenesis
biological effects
staining
Staining and Labeling

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Radiology Nuclear Medicine and imaging
  • Biophysics
  • Radiation

Cite this

Measurement of the relative proportion of symmetrical and asymmetrical chromosome-type interchanges induced by γ radiation in human-hamster hybrid cells. / Bahari, I. B.; Bedford, J. S.; Giaccia, A. J.; Stamato, T. D.

In: Radiation Research, Vol. 123, No. 1, 1990, p. 105-107.

Research output: Contribution to journalArticle

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