Measurement of androstenedione levels by an in-house radioimmunoassay.

M. S. Lo, M. L. Ng, Loo Ling Wu, B. A. Khalid

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

An in-house radioimmunoassay (RIA) for the measurement of androstenedione levels in serum was established and validated. Levels of androstenedione were measured by RIA using serum samples from various normal population groups and patients with congenital adrenal hyperplasia (CAH). Analytical recovery and linearity results were > 95%, while intra- and inter-assay CVs were < 10% and < 22% respectively. The assay sensitivity was 0.5 nmol/l or 25 fmol/tube. In normal population groups, the highest androstenedione levels were found in preterm neonates (1.6-12.4 nmol/l), followed by adult females (1.5-10.2 nmol/l), adult males (1.6-8.0 nmol/l) and term neonates (0.8-8.8 nmol/l), while the lowest values were observed in prepubertal children (0.5-3.4 nmol/l). There were no significant differences in diurnal variation and between follicular and luteal phases. The range of androstenedione levels in untreated or poorly controlled CAH patients (7.6-355.0 nmol/l, median 42.5 nmol/l, n = 20) were significantly higher (p < 0.001) than the upper normal limit of 3.4 nmol/L for prepubertal children. The normal androstenedione reference ranges for paediatric and adult groups have thus been established.

Original languageEnglish
Pages (from-to)53-58
Number of pages6
JournalThe Malaysian journal of pathology
Volume18
Issue number1
Publication statusPublished - Jun 1996

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Androstenedione
Radioimmunoassay
Congenital Adrenal Hyperplasia
Population Groups
Newborn Infant
Follicular Phase
Luteal Phase
Serum
Reference Values
Pediatrics

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Measurement of androstenedione levels by an in-house radioimmunoassay. / Lo, M. S.; Ng, M. L.; Wu, Loo Ling; Khalid, B. A.

In: The Malaysian journal of pathology, Vol. 18, No. 1, 06.1996, p. 53-58.

Research output: Contribution to journalArticle

Lo, M. S. ; Ng, M. L. ; Wu, Loo Ling ; Khalid, B. A. / Measurement of androstenedione levels by an in-house radioimmunoassay. In: The Malaysian journal of pathology. 1996 ; Vol. 18, No. 1. pp. 53-58.
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