Maximization of growth and storage of locally isolated lactobacillus salivarius subsp. salivarius with high stability and functionality

N. K M Salih, A. Hutari, W. S. Gaseem, Wan Mohtar Wan Yusoff

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

This study, conforms the protocol adopted to agree with bio-safety aspects of Lactobacillus salivarius subsp. salivarius including evaluation of viability during storage and incubation of free cells under starving conditions for optimization of various process parameters such as heat, high concentration of salt, inoculums' size, and pH have been studied. A time course experiment was conducted incorporating all the optimized parameters. This study improves the production, functionality and the enlargement of the commercial availability as one of the important probiotics in drugs, food and feed. Estimated optimum conditions established for the growth of Lactobacillus salivarius subsp. salivarius are as follows: pH =6.5; temperature = 37-40°C inoculums size =5 % (w/v) and salt concentration = 6.0% (w/v). The storage stability of the cultures was excellent in freeze-dried form and for cultures frozen at - 45°C or less for 10 months.

Original languageEnglish
Title of host publicationIFMBE Proceedings
Pages175-178
Number of pages4
Volume24
DOIs
Publication statusPublished - 2009
Event25th Southern Biomedical Engineering Conference 2009 - Miami, FL
Duration: 15 May 200917 May 2009

Other

Other25th Southern Biomedical Engineering Conference 2009
CityMiami, FL
Period15/5/0917/5/09

Fingerprint

Salts
Cells
Availability
Pharmaceutical Preparations
Experiments
Temperature
Hot Temperature
Probiotics

Keywords

  • Improving storage
  • L. salivarius subsp. salivarius
  • Probiotics
  • Survival
  • Validity

ASJC Scopus subject areas

  • Biomedical Engineering
  • Bioengineering

Cite this

Maximization of growth and storage of locally isolated lactobacillus salivarius subsp. salivarius with high stability and functionality. / Salih, N. K M; Hutari, A.; Gaseem, W. S.; Wan Yusoff, Wan Mohtar.

IFMBE Proceedings. Vol. 24 2009. p. 175-178.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Salih, NKM, Hutari, A, Gaseem, WS & Wan Yusoff, WM 2009, Maximization of growth and storage of locally isolated lactobacillus salivarius subsp. salivarius with high stability and functionality. in IFMBE Proceedings. vol. 24, pp. 175-178, 25th Southern Biomedical Engineering Conference 2009, Miami, FL, 15/5/09. https://doi.org/10.1007/978-3-642-01697-4_64
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