Marantodes pumilum leaves promote repair of osteoporotic fracture in postmenopausal sprague-dawley rats

Tijjani Rabiu Giaze, Ahmad Nazrun Shuid, Ima Nirwana Soelaiman, Norliza Muhammad, Mh Busra Fauzi, Azlan Mohd Arlamsyah, Norazlina Mohamed

Research output: Contribution to journalArticle

Abstract

Background and Objective: Marantodes pumilum var alata (MPva), a herb product used in managing female reproductive health problems and postmenopausal symptoms, is known to protect the bone against osteoporosis. Despite convincing evidence of its anti-osteoporotic properties, its role on osteoporotic fracture is yet to be investigated. In this study, the effects of aqueous leaf extract of MPva on fracture repair were studied in ovariectomized rats. Materials and Methods: Thirty female Sprague-Dawley rats grouped (n = 6) into Sham-operated (Sham), Ovariectomized control (OVXC), Estrogen treatment (ERT) and leaf treatments (MPv20 and MPv100) groups were ovariectomized (except the Sham). Eight weeks post-ovariectomy, the right tibiae of rats were fractured and fixed with titanium plates. The ERT group received 64.5 µg kgG1 per day p.o. estrogen (Premarin®) while the MPv20 and MPv100 groups received 20 mg and 100 mg kgG1 per day p.o. doses of MPva leaf extract, respectively. After 8 weeks treatment, rats were humanely euthanized and their tibiae were excised for investigation of callus bone morphometry, mineralization and biomechanical strength. Results: Callus bone volume (BVcallus) and volume fraction (BV/TVcallus) were found to be higher (p<0.05) in MPv20 and MPv100 compared to OVXC. Similar to ERT and Sham, bone mechanical strength parameters, maximum stress and maximum force, were significantly higher in both MPv20 and MPv100 groups compared to OVXC. Conclusion: Similar to estrogen treatment and healthy control, treatment with aqueous leaves extract of Mpva promotes fracture healing in postmenopausal osteoporotic rats by enhancing the volume of bone callus formed at fracture site and its subsequent remodeling. In view of its safety profile, Mpva leaves could be a suitable complementary medicine in the management of osteoporotic fracture in postmenopausal condition.

Original languageEnglish
Pages (from-to)973-980
Number of pages8
JournalInternational Journal of Pharmacology
Volume14
Issue number7
DOIs
Publication statusPublished - 1 Jan 2018

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Osteoporotic Fractures
Sprague Dawley Rats
Estrogens
Bony Callus
Bone and Bones
Tibia
Therapeutics
Conjugated (USP) Estrogens
Physiologic Calcification
Fracture Healing
Reproductive Health
Ovariectomy
Complementary Therapies
Titanium
Osteoporosis
Placebos
Safety

Keywords

  • Fracture-healing
  • Marantodes pumilum
  • Osteoporosis
  • Ovariectomized rats
  • Phytoestrogens

ASJC Scopus subject areas

  • Pharmacology

Cite this

Marantodes pumilum leaves promote repair of osteoporotic fracture in postmenopausal sprague-dawley rats. / Giaze, Tijjani Rabiu; Shuid, Ahmad Nazrun; Soelaiman, Ima Nirwana; Muhammad, Norliza; Busra Fauzi, Mh; Arlamsyah, Azlan Mohd; Mohamed, Norazlina.

In: International Journal of Pharmacology, Vol. 14, No. 7, 01.01.2018, p. 973-980.

Research output: Contribution to journalArticle

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AU - Muhammad, Norliza

AU - Busra Fauzi, Mh

AU - Arlamsyah, Azlan Mohd

AU - Mohamed, Norazlina

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AB - Background and Objective: Marantodes pumilum var alata (MPva), a herb product used in managing female reproductive health problems and postmenopausal symptoms, is known to protect the bone against osteoporosis. Despite convincing evidence of its anti-osteoporotic properties, its role on osteoporotic fracture is yet to be investigated. In this study, the effects of aqueous leaf extract of MPva on fracture repair were studied in ovariectomized rats. Materials and Methods: Thirty female Sprague-Dawley rats grouped (n = 6) into Sham-operated (Sham), Ovariectomized control (OVXC), Estrogen treatment (ERT) and leaf treatments (MPv20 and MPv100) groups were ovariectomized (except the Sham). Eight weeks post-ovariectomy, the right tibiae of rats were fractured and fixed with titanium plates. The ERT group received 64.5 µg kgG1 per day p.o. estrogen (Premarin®) while the MPv20 and MPv100 groups received 20 mg and 100 mg kgG1 per day p.o. doses of MPva leaf extract, respectively. After 8 weeks treatment, rats were humanely euthanized and their tibiae were excised for investigation of callus bone morphometry, mineralization and biomechanical strength. Results: Callus bone volume (BVcallus) and volume fraction (BV/TVcallus) were found to be higher (p<0.05) in MPv20 and MPv100 compared to OVXC. Similar to ERT and Sham, bone mechanical strength parameters, maximum stress and maximum force, were significantly higher in both MPv20 and MPv100 groups compared to OVXC. Conclusion: Similar to estrogen treatment and healthy control, treatment with aqueous leaves extract of Mpva promotes fracture healing in postmenopausal osteoporotic rats by enhancing the volume of bone callus formed at fracture site and its subsequent remodeling. In view of its safety profile, Mpva leaves could be a suitable complementary medicine in the management of osteoporotic fracture in postmenopausal condition.

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