Marantodes pumilum (Blume) kuntze inhibited secretion of lipopolysaccharide- and monosodium urate crystal-stimulated cytokines and plasma prostaglandin E2

Eldiza Puji Rahmi, Jamia Azdina Jamal, Kumolosasi Msi Endang, Juriyati Jalil, Nor Ashila Aladdin

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1 Citation (Scopus)

Abstract

Background: Marantodes pumilum is traditionally used for dysentery, gonorrhea, and sickness in the bones. Previous studies revealed its antibacterial and xanthine oxidase inhibitory activities. Objective: To evaluate the inhibitory effects of three M. pumilum varieties on the secretion of lipopolysaccharide (LPS)- and monosodium urate crystal (MSU)-induced cytokines and plasma prostaglandin E2 (PGE2) in vitro. Materials and Methods: The leaves and roots of M. pumilum var. alata (MPA), M. pumilum var. pumila (MPP), and M. pumilum var. lanceolata (MPL) were successively extracted with dichloromethane (DCM), methanol, and water. Human peripheral blood mononuclear cells and ELISA technique were used for the cytokine assay, whereas human plasma and radioimmunoassay technique were used in the PGE2assay. Flavonoids content was determined using a reversed-phase high-performance liquid chromatography. Results: DCM extract of MPL roots showed the highest inhibition of LPS-stimulated cytokine secretion with IC50values of 29.87, 7.62, 5.84, 25.33, and 5.40 μ g/mL for interleukin (IL)-1α , IL-1β , IL-6, IL-8, and tumor necrosis factor (TNF)-α , respectively; while that of plasma PGE2 secretion was given by DCM extract of MPP roots (IC5031.10 μ g/mL). Similarly, the DCM extract of MPL roots demonstrated the highest inhibition against MSU-stimulated IL-1α , IL-1β , IL-6, IL-8, TNF-α , and PGE2 secretion with IC50values of 11.2, 8.92, 12.29, 49.51, 9.60, and 31.58 μ g/mL, respectively. Apigenin in DCM extracts of MPL (0.051 mg/g) and MPP (0.064 mg/g) roots could be responsible for the strong inhibitory activity against IL-1β , IL-6, TNF-α , and PGE2. Conclusion: The results suggested that DCM extracts of MPL and MPP roots are potential anti-inflammatory agents by inhibiting the secretion of LPS- and MSU-stimulated pro-inflammatory cytokines and PGE2.

Original languageEnglish
Pages (from-to)S578-S586
JournalPharmacognosy Magazine
Volume13
Issue number51
DOIs
Publication statusPublished - 2017

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Methylene Chloride
Uric Acid
Dinoprostone
Lipopolysaccharides
Interleukin-1
Cytokines
Interleukin-6
Tumor Necrosis Factor-alpha
Interleukin-8
Apigenin
Dysentery
Xanthine Oxidase
Gonorrhea
Reverse-Phase Chromatography
Flavonoids
Radioimmunoassay
Methanol
Blood Cells
Anti-Inflammatory Agents
Enzyme-Linked Immunosorbent Assay

Keywords

  • Lipopolysaccharide
  • Marantodes pumilum
  • monosodium urate crystals
  • pro-inflammatory cytokines
  • prostaglandin E

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Drug Discovery

Cite this

@article{31750a186032417b9f3a4aeef77ec01c,
title = "Marantodes pumilum (Blume) kuntze inhibited secretion of lipopolysaccharide- and monosodium urate crystal-stimulated cytokines and plasma prostaglandin E2",
abstract = "Background: Marantodes pumilum is traditionally used for dysentery, gonorrhea, and sickness in the bones. Previous studies revealed its antibacterial and xanthine oxidase inhibitory activities. Objective: To evaluate the inhibitory effects of three M. pumilum varieties on the secretion of lipopolysaccharide (LPS)- and monosodium urate crystal (MSU)-induced cytokines and plasma prostaglandin E2 (PGE2) in vitro. Materials and Methods: The leaves and roots of M. pumilum var. alata (MPA), M. pumilum var. pumila (MPP), and M. pumilum var. lanceolata (MPL) were successively extracted with dichloromethane (DCM), methanol, and water. Human peripheral blood mononuclear cells and ELISA technique were used for the cytokine assay, whereas human plasma and radioimmunoassay technique were used in the PGE2assay. Flavonoids content was determined using a reversed-phase high-performance liquid chromatography. Results: DCM extract of MPL roots showed the highest inhibition of LPS-stimulated cytokine secretion with IC50values of 29.87, 7.62, 5.84, 25.33, and 5.40 μ g/mL for interleukin (IL)-1α , IL-1β , IL-6, IL-8, and tumor necrosis factor (TNF)-α , respectively; while that of plasma PGE2 secretion was given by DCM extract of MPP roots (IC5031.10 μ g/mL). Similarly, the DCM extract of MPL roots demonstrated the highest inhibition against MSU-stimulated IL-1α , IL-1β , IL-6, IL-8, TNF-α , and PGE2 secretion with IC50values of 11.2, 8.92, 12.29, 49.51, 9.60, and 31.58 μ g/mL, respectively. Apigenin in DCM extracts of MPL (0.051 mg/g) and MPP (0.064 mg/g) roots could be responsible for the strong inhibitory activity against IL-1β , IL-6, TNF-α , and PGE2. Conclusion: The results suggested that DCM extracts of MPL and MPP roots are potential anti-inflammatory agents by inhibiting the secretion of LPS- and MSU-stimulated pro-inflammatory cytokines and PGE2.",
keywords = "Lipopolysaccharide, Marantodes pumilum, monosodium urate crystals, pro-inflammatory cytokines, prostaglandin E",
author = "Rahmi, {Eldiza Puji} and Jamal, {Jamia Azdina} and Endang, {Kumolosasi Msi} and Juriyati Jalil and Aladdin, {Nor Ashila}",
year = "2017",
doi = "10.4103/pm.pm_35_17",
language = "English",
volume = "13",
pages = "S578--S586",
journal = "Pharmacognosy Magazine",
issn = "0973-1296",
publisher = "Medknow Publications and Media Pvt. Ltd",
number = "51",

}

TY - JOUR

T1 - Marantodes pumilum (Blume) kuntze inhibited secretion of lipopolysaccharide- and monosodium urate crystal-stimulated cytokines and plasma prostaglandin E2

AU - Rahmi, Eldiza Puji

AU - Jamal, Jamia Azdina

AU - Endang, Kumolosasi Msi

AU - Jalil, Juriyati

AU - Aladdin, Nor Ashila

PY - 2017

Y1 - 2017

N2 - Background: Marantodes pumilum is traditionally used for dysentery, gonorrhea, and sickness in the bones. Previous studies revealed its antibacterial and xanthine oxidase inhibitory activities. Objective: To evaluate the inhibitory effects of three M. pumilum varieties on the secretion of lipopolysaccharide (LPS)- and monosodium urate crystal (MSU)-induced cytokines and plasma prostaglandin E2 (PGE2) in vitro. Materials and Methods: The leaves and roots of M. pumilum var. alata (MPA), M. pumilum var. pumila (MPP), and M. pumilum var. lanceolata (MPL) were successively extracted with dichloromethane (DCM), methanol, and water. Human peripheral blood mononuclear cells and ELISA technique were used for the cytokine assay, whereas human plasma and radioimmunoassay technique were used in the PGE2assay. Flavonoids content was determined using a reversed-phase high-performance liquid chromatography. Results: DCM extract of MPL roots showed the highest inhibition of LPS-stimulated cytokine secretion with IC50values of 29.87, 7.62, 5.84, 25.33, and 5.40 μ g/mL for interleukin (IL)-1α , IL-1β , IL-6, IL-8, and tumor necrosis factor (TNF)-α , respectively; while that of plasma PGE2 secretion was given by DCM extract of MPP roots (IC5031.10 μ g/mL). Similarly, the DCM extract of MPL roots demonstrated the highest inhibition against MSU-stimulated IL-1α , IL-1β , IL-6, IL-8, TNF-α , and PGE2 secretion with IC50values of 11.2, 8.92, 12.29, 49.51, 9.60, and 31.58 μ g/mL, respectively. Apigenin in DCM extracts of MPL (0.051 mg/g) and MPP (0.064 mg/g) roots could be responsible for the strong inhibitory activity against IL-1β , IL-6, TNF-α , and PGE2. Conclusion: The results suggested that DCM extracts of MPL and MPP roots are potential anti-inflammatory agents by inhibiting the secretion of LPS- and MSU-stimulated pro-inflammatory cytokines and PGE2.

AB - Background: Marantodes pumilum is traditionally used for dysentery, gonorrhea, and sickness in the bones. Previous studies revealed its antibacterial and xanthine oxidase inhibitory activities. Objective: To evaluate the inhibitory effects of three M. pumilum varieties on the secretion of lipopolysaccharide (LPS)- and monosodium urate crystal (MSU)-induced cytokines and plasma prostaglandin E2 (PGE2) in vitro. Materials and Methods: The leaves and roots of M. pumilum var. alata (MPA), M. pumilum var. pumila (MPP), and M. pumilum var. lanceolata (MPL) were successively extracted with dichloromethane (DCM), methanol, and water. Human peripheral blood mononuclear cells and ELISA technique were used for the cytokine assay, whereas human plasma and radioimmunoassay technique were used in the PGE2assay. Flavonoids content was determined using a reversed-phase high-performance liquid chromatography. Results: DCM extract of MPL roots showed the highest inhibition of LPS-stimulated cytokine secretion with IC50values of 29.87, 7.62, 5.84, 25.33, and 5.40 μ g/mL for interleukin (IL)-1α , IL-1β , IL-6, IL-8, and tumor necrosis factor (TNF)-α , respectively; while that of plasma PGE2 secretion was given by DCM extract of MPP roots (IC5031.10 μ g/mL). Similarly, the DCM extract of MPL roots demonstrated the highest inhibition against MSU-stimulated IL-1α , IL-1β , IL-6, IL-8, TNF-α , and PGE2 secretion with IC50values of 11.2, 8.92, 12.29, 49.51, 9.60, and 31.58 μ g/mL, respectively. Apigenin in DCM extracts of MPL (0.051 mg/g) and MPP (0.064 mg/g) roots could be responsible for the strong inhibitory activity against IL-1β , IL-6, TNF-α , and PGE2. Conclusion: The results suggested that DCM extracts of MPL and MPP roots are potential anti-inflammatory agents by inhibiting the secretion of LPS- and MSU-stimulated pro-inflammatory cytokines and PGE2.

KW - Lipopolysaccharide

KW - Marantodes pumilum

KW - monosodium urate crystals

KW - pro-inflammatory cytokines

KW - prostaglandin E

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U2 - 10.4103/pm.pm_35_17

DO - 10.4103/pm.pm_35_17

M3 - Article

AN - SCOPUS:85032986115

VL - 13

SP - S578-S586

JO - Pharmacognosy Magazine

JF - Pharmacognosy Magazine

SN - 0973-1296

IS - 51

ER -