Malaysian gelam honey reduces oxidative damage and modulates antioxidant enzyme activities in young and middle aged rats

Lee Ke Yao, Sarah Liana Abdul Razak, Nazhirah Ismail, Ng Chun Fai, Mohd Hafis Asyraf, Muhamad Asgar, Nursyahirah Mohd Sharif, Goon Jo Aan, Zakiah Jubri

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Honey contains antioxidants such as phenolic compounds that prevent cellular oxidative damage that lead to aging, diseases and death. The aim of this study is to determine the effect of local gelam honey supplementation on oxidative status of young and middle-aged rats. Thirty-six male Sprague-Dawley rats were divided into two age groups; young (2 months) and middle aged (9 months). Each group was further divided into three groups; fed with plain water (control), supplemented with 2.5 and 5.0 g/kg of gelam honey for 30 days. DNA damage level was determined by comet assay, plasma malondialdehyde by high performance liquid chromatography and antioxidant enzymes activities (superoxide dismutase; SOD, glutathione peroxidase; GPx and catalase; CAT) were determined in the erythrocytes and liver. Results showed that supplementation of gelam honey reduced DNA damage, MDA level and GPx activity in the liver of both age groups. But erythrocytes GPx activity in young rats increased markedly with 5 g/kg of gelam honey supplementation. Liver and erythrocytes CAT activities decreased in both age groups when two different doses were used. Liver SOD activity also decreased in young rats supplemented with 5 g/kg of gelam honey. In conclusion, gelam honey reduces oxidative damage of young and middle aged rats by modulating antioxidant enzyme activities which was more prominent at higher concentration (5 g/kg body weight) compared to lower concentration (2.5 g/kg body weight).

Original languageEnglish
Pages (from-to)5618-5625
Number of pages8
JournalJournal of Medicinal Plant Research
Volume5
Issue number23
Publication statusPublished - 23 Oct 2011

Fingerprint

middle-aged adults
Honey
honey
Antioxidants
enzyme activity
antioxidants
rats
Enzymes
superoxide dismutase
liver
erythrocytes
Age Groups
Erythrocytes
Liver
DNA damage
DNA Damage
Body Weight
Comet Assay
body weight
Glutathione Peroxidase

Keywords

  • Antioxidant enzymes
  • DNA damage
  • Gelam honey
  • Malondialdehyde

ASJC Scopus subject areas

  • Pharmacology
  • Complementary and alternative medicine
  • Drug Discovery
  • Plant Science
  • Pharmaceutical Science

Cite this

Malaysian gelam honey reduces oxidative damage and modulates antioxidant enzyme activities in young and middle aged rats. / Yao, Lee Ke; Razak, Sarah Liana Abdul; Ismail, Nazhirah; Fai, Ng Chun; Asyraf, Mohd Hafis; Asgar, Muhamad; Sharif, Nursyahirah Mohd; Jo Aan, Goon; Jubri, Zakiah.

In: Journal of Medicinal Plant Research, Vol. 5, No. 23, 23.10.2011, p. 5618-5625.

Research output: Contribution to journalArticle

Yao, Lee Ke ; Razak, Sarah Liana Abdul ; Ismail, Nazhirah ; Fai, Ng Chun ; Asyraf, Mohd Hafis ; Asgar, Muhamad ; Sharif, Nursyahirah Mohd ; Jo Aan, Goon ; Jubri, Zakiah. / Malaysian gelam honey reduces oxidative damage and modulates antioxidant enzyme activities in young and middle aged rats. In: Journal of Medicinal Plant Research. 2011 ; Vol. 5, No. 23. pp. 5618-5625.
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