Lysozyme transmission through polymeric based ultrafiltration membrane: Effect of pH and ionic strength

Sofiah Hamzah, Nora'aini Ali, Marinah Mohd Ariffin, Abdul Wahab Mohammad

Research output: Contribution to journalArticle

Abstract

Fractionation and purification of complex protein mixture has becomes a great interest and has attracted a considerable amount of attention in recent years. This study aimed is to demonstrate the factors influencing the lysozyme separation through polyethersulfone ultrafiltration (UF) membrane. Asymmetric UF membrane with 15% polymer composition (UF15) was developed via phase inversion technique. Membrane was characterized in term of morphology, membrane surface charge and molecular weight cut-off to ensure its suitability for lysozyme separation. Effect of pH on the transmission of lysozyme through the UF15 membrane has been examined at different pH (5, 7, 9, 11 and 13) and ionic strength (0.1M, 0.2M and 0.3M). Results displayed that at optimum pressure 3 bars, permeation with pH 9 and 0.1M ionic strength of feed solution obtained the optimum flux and lysozyme transmission for about 36.6 x 10-5 m3/m2and 92.9%, respectively. This study has proved that pH and ionic strength were found to be greatly affected the lysozyme transmission and promoted the lysozyme separation to a significant degree.

Original languageEnglish
Pages (from-to)2315-2321
Number of pages7
JournalARPN Journal of Engineering and Applied Sciences
Volume9
Issue number11
Publication statusPublished - 1 Jan 2014

Fingerprint

Ultrafiltration
Ionic strength
Enzymes
Membranes
Surface charge
Fractionation
Permeation
Purification
Molecular weight
Fluxes
Proteins
Polymers
Chemical analysis

Keywords

  • Ionic strength
  • Lysozyme
  • Membranes
  • PH
  • Protein
  • Ultrafiltration

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Lysozyme transmission through polymeric based ultrafiltration membrane : Effect of pH and ionic strength. / Hamzah, Sofiah; Ali, Nora'aini; Ariffin, Marinah Mohd; Mohammad, Abdul Wahab.

In: ARPN Journal of Engineering and Applied Sciences, Vol. 9, No. 11, 01.01.2014, p. 2315-2321.

Research output: Contribution to journalArticle

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