Abstract
Fractionation and purification of complex protein mixture has becomes a great interest and has attracted a considerable amount of attention in recent years. This study aimed is to demonstrate the factors influencing the lysozyme separation through polyethersulfone ultrafiltration (UF) membrane. Asymmetric UF membrane with 15% polymer composition (UF15) was developed via phase inversion technique. Membrane was characterized in term of morphology, membrane surface charge and molecular weight cut-off to ensure its suitability for lysozyme separation. Effect of pH on the transmission of lysozyme through the UF15 membrane has been examined at different pH (5, 7, 9, 11 and 13) and ionic strength (0.1M, 0.2M and 0.3M). Results displayed that at optimum pressure 3 bars, permeation with pH 9 and 0.1M ionic strength of feed solution obtained the optimum flux and lysozyme transmission for about 36.6 x 10-5 m3/m2and 92.9%, respectively. This study has proved that pH and ionic strength were found to be greatly affected the lysozyme transmission and promoted the lysozyme separation to a significant degree.
| Original language | English |
|---|---|
| Pages (from-to) | 2315-2321 |
| Number of pages | 7 |
| Journal | ARPN Journal of Engineering and Applied Sciences |
| Volume | 9 |
| Issue number | 11 |
| Publication status | Published - 1 Jan 2014 |
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Keywords
- Ionic strength
- Lysozyme
- Membranes
- PH
- Protein
- Ultrafiltration
ASJC Scopus subject areas
- Engineering(all)
Cite this
Lysozyme transmission through polymeric based ultrafiltration membrane : Effect of pH and ionic strength. / Hamzah, Sofiah; Ali, Nora'aini; Ariffin, Marinah Mohd; Mohammad, Abdul Wahab.
In: ARPN Journal of Engineering and Applied Sciences, Vol. 9, No. 11, 01.01.2014, p. 2315-2321.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Lysozyme transmission through polymeric based ultrafiltration membrane
T2 - Effect of pH and ionic strength
AU - Hamzah, Sofiah
AU - Ali, Nora'aini
AU - Ariffin, Marinah Mohd
AU - Mohammad, Abdul Wahab
PY - 2014/1/1
Y1 - 2014/1/1
N2 - Fractionation and purification of complex protein mixture has becomes a great interest and has attracted a considerable amount of attention in recent years. This study aimed is to demonstrate the factors influencing the lysozyme separation through polyethersulfone ultrafiltration (UF) membrane. Asymmetric UF membrane with 15% polymer composition (UF15) was developed via phase inversion technique. Membrane was characterized in term of morphology, membrane surface charge and molecular weight cut-off to ensure its suitability for lysozyme separation. Effect of pH on the transmission of lysozyme through the UF15 membrane has been examined at different pH (5, 7, 9, 11 and 13) and ionic strength (0.1M, 0.2M and 0.3M). Results displayed that at optimum pressure 3 bars, permeation with pH 9 and 0.1M ionic strength of feed solution obtained the optimum flux and lysozyme transmission for about 36.6 x 10-5 m3/m2and 92.9%, respectively. This study has proved that pH and ionic strength were found to be greatly affected the lysozyme transmission and promoted the lysozyme separation to a significant degree.
AB - Fractionation and purification of complex protein mixture has becomes a great interest and has attracted a considerable amount of attention in recent years. This study aimed is to demonstrate the factors influencing the lysozyme separation through polyethersulfone ultrafiltration (UF) membrane. Asymmetric UF membrane with 15% polymer composition (UF15) was developed via phase inversion technique. Membrane was characterized in term of morphology, membrane surface charge and molecular weight cut-off to ensure its suitability for lysozyme separation. Effect of pH on the transmission of lysozyme through the UF15 membrane has been examined at different pH (5, 7, 9, 11 and 13) and ionic strength (0.1M, 0.2M and 0.3M). Results displayed that at optimum pressure 3 bars, permeation with pH 9 and 0.1M ionic strength of feed solution obtained the optimum flux and lysozyme transmission for about 36.6 x 10-5 m3/m2and 92.9%, respectively. This study has proved that pH and ionic strength were found to be greatly affected the lysozyme transmission and promoted the lysozyme separation to a significant degree.
KW - Ionic strength
KW - Lysozyme
KW - Membranes
KW - PH
KW - Protein
KW - Ultrafiltration
UR - http://www.scopus.com/inward/record.url?scp=85068903922&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85068903922&partnerID=8YFLogxK
M3 - Article
VL - 9
SP - 2315
EP - 2321
JO - ARPN Journal of Engineering and Applied Sciences
JF - ARPN Journal of Engineering and Applied Sciences
SN - 1819-6608
IS - 11
ER -