Lycopene-rich fractions derived from pink guava by-product and their potential activity towards hydrogen peroxide-induced cellular and DNA damage

Kin Weng Kong, Nor Fadilah Rajab, K. Nagendra Prasad, Amin Ismail, Masturah Markom, Chin Ping Tan

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Effects of solvent and supercritical carbon dioxide (SC-CO2) extraction on antioxidant and cytotoxic activities of lycopene-rich fractions of decanted pink guava by-product (decanter) were determined with lycopene-equivalent antioxidant capacity, β-carotene bleaching and MTT (3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide) assays. Extraction with SC-CO2 gave a higher yield than solvent extraction (3.15 vs. 0.68mg/100g dried decanter, corresponding to 42.99 and 33.63mg of lycopene). No cytotoxicity was found in Chang liver cells supplemented with either extracts (6.25-200μg/ml). Solvent extract at 25μg/ml (2.32μM lycopene) and SC-CO2 extract at 200μg/ml (5.09μM lycopene) had protective effect against hydrogen peroxide-induced cytotoxicity. However, only high concentrations of solvent extract (200μg/ml; lycopene=18.65μM) or lycopene standard (10μM) protected cells against DNA damage. Supercritical fluid extraction demonstrated a higher yield in lycopene-rich fraction from decanter. These fractions have the potential to be developed as a functional ingredient to prevent oxidative stress and other related diseases.

Original languageEnglish
Pages (from-to)1142-1148
Number of pages7
JournalFood Chemistry
Volume123
Issue number4
DOIs
Publication statusPublished - Dec 2010

Fingerprint

Psidium
guavas
lycopene
DNA damage
Hydrogen Peroxide
DNA Damage
hydrogen peroxide
Byproducts
DNA
carbon dioxide
extracts
Cytotoxicity
cytotoxicity
Antioxidants
Supercritical Fluid Chromatography
Supercritical fluid extraction
antioxidants
supercritical fluid extraction
Oxidative stress
tetrazolium

Keywords

  • By-product
  • Comet assay
  • Cytotoxicity
  • DNA damage
  • Lycopene
  • Psidium guajava

ASJC Scopus subject areas

  • Food Science
  • Analytical Chemistry

Cite this

Lycopene-rich fractions derived from pink guava by-product and their potential activity towards hydrogen peroxide-induced cellular and DNA damage. / Kong, Kin Weng; Rajab, Nor Fadilah; Nagendra Prasad, K.; Ismail, Amin; Markom, Masturah; Tan, Chin Ping.

In: Food Chemistry, Vol. 123, No. 4, 12.2010, p. 1142-1148.

Research output: Contribution to journalArticle

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