Potensi Anti-Hiperglisemia Lunasia amara dan Kesannya terhadap Parameter Kesuburan Tikus Jantan Teraruh-Diabetes

Translated title of the contribution: Lunasia amara anti-hyperglycemic potential and its effect towards fertility parameters in diabetes-induced male rat

Nor Raidah Rahmat, Amira Kamalrudin, Mohd Shazrul Fazry Sa`Ariwijaya, Mahanem Mat Noor

Research output: Contribution to journalArticle

Abstract

Lunasia amara has been used in folk medicine to treat diabetes mellitus. It has been reported to have pro-fertility effects in normal male rats at a dose of 60 mg/kg body weight. This study was conducted to investigate the potential of L. amara aqueous extract as an anti-hyperglycemic agent and its ability to improve fertility parameters in diabetes-induced male rats. Six groups (n=5) of diabetes-induced rats were force-fed with L. amara aqueous extract (60, 120, 250, and 500 mg/kg body weight), 500 mg/kg body weight metformin, and distilled water, respectively, for seven consecutive days. Whereas, the seventh group is the normal control (n=5) without diabetic induction given distilled water (1 mL/rat). Doses of 250, and 500 mg/kg body weight L. amara extract were found to be effective for anti-hyperglycemic activity when given to diabetes-induced rats. Hence, only these two doses were used to further study the effect of L. amara on sperm quality and testis histology in diabetes-induced rats. The effect of testicular 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG) uptake in diabetes-induced rats was studied only at the dose of 500 mg/kg body weight L. amara. After 30 days of treatment, 250, and 500 mg/kg body weight L. amara were both found to inhibit all of the male fertility parameters studied despite their ability to lower the blood glucose level. Furthermore, testicular cells of diabetes-induced rats treated with 500 mg/kg body weight L. amara showed poor 2-NBDG uptake compared to control groups. The findings indicate that the anti-hyperglycemic mechanism of L. amara neither improves insulin function in term of 2-NBDG uptake into the testis nor contributes to the improvement of male fertility parameters in diabetes-induced rats.

Original languageMalay
Pages (from-to)103-109
Number of pages7
JournalSains Malaysiana
Volume48
Issue number1
DOIs
Publication statusPublished - 1 Jan 2019

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Fertility
Body Weight
Testis
Fertility Agents
Water
Metformin
Traditional Medicine
Blood Glucose
Spermatozoa
Histology
Diabetes Mellitus
Insulin
Control Groups
2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose

ASJC Scopus subject areas

  • General

Cite this

Potensi Anti-Hiperglisemia Lunasia amara dan Kesannya terhadap Parameter Kesuburan Tikus Jantan Teraruh-Diabetes. / Rahmat, Nor Raidah; Kamalrudin, Amira; Sa`Ariwijaya, Mohd Shazrul Fazry; Mat Noor, Mahanem.

In: Sains Malaysiana, Vol. 48, No. 1, 01.01.2019, p. 103-109.

Research output: Contribution to journalArticle

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