Low dose of tocotrienols protects osteoblasts against oxidative stress

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Abstract

Aims: Vitamin E is an antioxidant that may protect bone against oxidative stress-induced osteoporosis. This in vitro study was conducted to determine the protective effects of α-tocopherol and γ-tocotrienol on osteoblasts, the bone forming cells, against oxidative stress. Materials and Methods. Toxicity tests were carried out on hydrogen peroxide (H 2O 2), α-tocopherol and γ-tocotrienol and their inhibitory concentration 50 (IC 50) on osteoblasts were determined if any. Osteoblast cultures were then pretreated with different concentrations of α-tocopherol or γ-tocotrienol for 24 hours before incubated with the IC 50 of H 2O 2 for 2 hours. Cell viability was determined by using MTS assay to compare the protective effects of both vitamin E on osteoblast exposed to H 2O 2. Results. The IC 50 after 2 hours and 24 hours incubation time for H 2O 2 were 490 μM and 280 μM respectively. γ-Tocotrienol was found to be toxic to osteoblasts with the IC 50 of 290 μM after 24 hours incubation time while α-tocopherol was not toxic to osteoblasts at any doses. However, γ-tocotrienol was able to protect osteoblasts from H 2O 2 toxicity at low concentration (1 μM), whereras α-tocopherol was not able to offer protection against H 2O 2 toxicity. Conclusions. γ-tocotrienol was found to be toxic to osteoblasts at high concentrations but at much lower concentration, it has better antioxidant activity than α-tocopherol to protect osteoblasts from oxidative stress.

Original languageEnglish
Pages (from-to)533-538
Number of pages6
JournalClinica Terapeutica
Volume162
Issue number6
Publication statusPublished - 2011

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Tocotrienols
Osteoblasts
Oxidative Stress
Tocopherols
Inhibitory Concentration 50
Poisons
Vitamin E
Antioxidants
Bone and Bones
Toxicity Tests
Hydrogen Peroxide
Osteoporosis
Cell Survival

Keywords

  • Osteoblast
  • Osteoporosis
  • Oxidative stress
  • Tocotrienol
  • Vitamin E

ASJC Scopus subject areas

  • Medicine(all)

Cite this

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title = "Low dose of tocotrienols protects osteoblasts against oxidative stress",
abstract = "Aims: Vitamin E is an antioxidant that may protect bone against oxidative stress-induced osteoporosis. This in vitro study was conducted to determine the protective effects of α-tocopherol and γ-tocotrienol on osteoblasts, the bone forming cells, against oxidative stress. Materials and Methods. Toxicity tests were carried out on hydrogen peroxide (H 2O 2), α-tocopherol and γ-tocotrienol and their inhibitory concentration 50 (IC 50) on osteoblasts were determined if any. Osteoblast cultures were then pretreated with different concentrations of α-tocopherol or γ-tocotrienol for 24 hours before incubated with the IC 50 of H 2O 2 for 2 hours. Cell viability was determined by using MTS assay to compare the protective effects of both vitamin E on osteoblast exposed to H 2O 2. Results. The IC 50 after 2 hours and 24 hours incubation time for H 2O 2 were 490 μM and 280 μM respectively. γ-Tocotrienol was found to be toxic to osteoblasts with the IC 50 of 290 μM after 24 hours incubation time while α-tocopherol was not toxic to osteoblasts at any doses. However, γ-tocotrienol was able to protect osteoblasts from H 2O 2 toxicity at low concentration (1 μM), whereras α-tocopherol was not able to offer protection against H 2O 2 toxicity. Conclusions. γ-tocotrienol was found to be toxic to osteoblasts at high concentrations but at much lower concentration, it has better antioxidant activity than α-tocopherol to protect osteoblasts from oxidative stress.",
keywords = "Osteoblast, Osteoporosis, Oxidative stress, Tocotrienol, Vitamin E",
author = "Nizar, {A. M.} and Shuid, {Ahmad Nazrun} and Norazlina Mohamed and Norliza Muhammad and Soelaiman, {Ima Nirwana}",
year = "2011",
language = "English",
volume = "162",
pages = "533--538",
journal = "Clinica Terapeutica",
issn = "0009-9074",
publisher = "Societa Editrice Universo",
number = "6",

}

TY - JOUR

T1 - Low dose of tocotrienols protects osteoblasts against oxidative stress

AU - Nizar, A. M.

AU - Shuid, Ahmad Nazrun

AU - Mohamed, Norazlina

AU - Muhammad, Norliza

AU - Soelaiman, Ima Nirwana

PY - 2011

Y1 - 2011

N2 - Aims: Vitamin E is an antioxidant that may protect bone against oxidative stress-induced osteoporosis. This in vitro study was conducted to determine the protective effects of α-tocopherol and γ-tocotrienol on osteoblasts, the bone forming cells, against oxidative stress. Materials and Methods. Toxicity tests were carried out on hydrogen peroxide (H 2O 2), α-tocopherol and γ-tocotrienol and their inhibitory concentration 50 (IC 50) on osteoblasts were determined if any. Osteoblast cultures were then pretreated with different concentrations of α-tocopherol or γ-tocotrienol for 24 hours before incubated with the IC 50 of H 2O 2 for 2 hours. Cell viability was determined by using MTS assay to compare the protective effects of both vitamin E on osteoblast exposed to H 2O 2. Results. The IC 50 after 2 hours and 24 hours incubation time for H 2O 2 were 490 μM and 280 μM respectively. γ-Tocotrienol was found to be toxic to osteoblasts with the IC 50 of 290 μM after 24 hours incubation time while α-tocopherol was not toxic to osteoblasts at any doses. However, γ-tocotrienol was able to protect osteoblasts from H 2O 2 toxicity at low concentration (1 μM), whereras α-tocopherol was not able to offer protection against H 2O 2 toxicity. Conclusions. γ-tocotrienol was found to be toxic to osteoblasts at high concentrations but at much lower concentration, it has better antioxidant activity than α-tocopherol to protect osteoblasts from oxidative stress.

AB - Aims: Vitamin E is an antioxidant that may protect bone against oxidative stress-induced osteoporosis. This in vitro study was conducted to determine the protective effects of α-tocopherol and γ-tocotrienol on osteoblasts, the bone forming cells, against oxidative stress. Materials and Methods. Toxicity tests were carried out on hydrogen peroxide (H 2O 2), α-tocopherol and γ-tocotrienol and their inhibitory concentration 50 (IC 50) on osteoblasts were determined if any. Osteoblast cultures were then pretreated with different concentrations of α-tocopherol or γ-tocotrienol for 24 hours before incubated with the IC 50 of H 2O 2 for 2 hours. Cell viability was determined by using MTS assay to compare the protective effects of both vitamin E on osteoblast exposed to H 2O 2. Results. The IC 50 after 2 hours and 24 hours incubation time for H 2O 2 were 490 μM and 280 μM respectively. γ-Tocotrienol was found to be toxic to osteoblasts with the IC 50 of 290 μM after 24 hours incubation time while α-tocopherol was not toxic to osteoblasts at any doses. However, γ-tocotrienol was able to protect osteoblasts from H 2O 2 toxicity at low concentration (1 μM), whereras α-tocopherol was not able to offer protection against H 2O 2 toxicity. Conclusions. γ-tocotrienol was found to be toxic to osteoblasts at high concentrations but at much lower concentration, it has better antioxidant activity than α-tocopherol to protect osteoblasts from oxidative stress.

KW - Osteoblast

KW - Osteoporosis

KW - Oxidative stress

KW - Tocotrienol

KW - Vitamin E

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