Low-dose nicotine exposure induced the oxidative damage of reproductive organs and altered the sperm characteristics of adolescent male rats

Siti Balkis Budin, Jia Hui Kho, Jia Hui Lee, Anand Ramalingam, Fatin Farhana Jubaidi, Elda Surhaida Latif, Satirah Zainalabidin, Taib Izatus Shima, Jamaludin Mohamed

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background: Nicotine is a major toxic and hazardous component of cigarette smoke, and it has been widely used in nicotine replacement therapy (NRT). This study was aimed to investigate the effects of chronic low-dose nicotine on sperm characteristics and reproductive organ integrity in adolescent male Sprague–Dawley rats. Methods: Twelve rats were equally divided into two groups. Group I received normal saline, and group II received 0.6 mg/kg body weight nicotine intraperitoneally for 28 consecutive days. At the end of the experimental period, sperm was collected for sperm characteristic evaluation, and the testes and prostate were isolated for biochemical and morphological analysis. The effects of nicotine on the body and reproductive organ weights of the animals were evaluated. Results: Chronic nicotine treatment significantly (P < 0.05) altered the sperm count, motility, viability, and morphology, and remarkably increased the malondialdehyde (P < 0.001) and advanced oxidation protein product (P < 0.05) levels in the testes and prostate of nicotine-treated group compared to control group. Moreover, nicotine caused a significant decrease (P < 0.05) in the superoxide dismutase activity of the testes. No significant differences were observed in the reduced glutathione level in both of the testes and prostate of nicotine group compared with control group. Nicotine also induced histopathological alteration in the testes. Conclusion: A low-dose nicotine exposure at 0.6 mg/kg caused detrimental effects on sperm characteristics and induced oxidative stress in the testes and prostate.

Original languageEnglish
Pages (from-to)50-57
Number of pages8
JournalMalaysian Journal of Medical Sciences
Volume24
Issue number6
DOIs
Publication statusPublished - 1 Nov 2017

Fingerprint

Nicotine
Spermatozoa
Testis
Prostate
Advanced Oxidation Protein Products
Control Groups
Sperm Count
Organ Size
Sperm Motility
Poisons
Malondialdehyde
Smoke
Tobacco Products
Superoxide Dismutase
Glutathione
Oxidative Stress
Body Weight

Keywords

  • Infertility
  • Male
  • Prostate
  • Reproductive
  • Sperm
  • Testes

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Low-dose nicotine exposure induced the oxidative damage of reproductive organs and altered the sperm characteristics of adolescent male rats. / Budin, Siti Balkis; Kho, Jia Hui; Lee, Jia Hui; Ramalingam, Anand; Jubaidi, Fatin Farhana; Latif, Elda Surhaida; Zainalabidin, Satirah; Izatus Shima, Taib; Mohamed, Jamaludin.

In: Malaysian Journal of Medical Sciences, Vol. 24, No. 6, 01.11.2017, p. 50-57.

Research output: Contribution to journalArticle

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AU - Jubaidi, Fatin Farhana

AU - Latif, Elda Surhaida

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