Low dose Marantodes pumilum leaf and roots extracts preserved bone structure in ovariectomized rats

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Abstract

Background: Marantodes pumilum var. alata, a phytoestrogen-rich herb, has been reported to exert protection on bone of estrogen-deficient animals against osteoporosis. However, comparative osteo-protective activity of its leaf and root extracts has not been fully elucidated. Objective: The aim of this study was to investigate and compare the osteo-protective effects of aqueous leaf and root extracts of Marantodes pumilum var alata in ovariectomized rat model. Methodology: Twenty-seven female rats were divided into nine groups: sham-operated (Sham); ovariectomized control (OVXC); 64.5 μg kg-1 day-1 estrogen treatment (ERT); 20 mg kg-1 day-1 (MPv20), 50 mg kg-1 day-1 (MPv50) and 100 mg kg-1 day-1 (MPv100) doses leaf extract treatments and; 20 mg kg-1 day-1 (MPr20), 50 mg kg-1 day-1 (MPr50) and 100 mg kg-1 day-1 (MPr100) doses root extract treatment groups. After 8 weeks treatment period, the left femora were excised and investigated using Micro-computed tomography (μ-CT). Results were analysed using one-way ANOVA and Tukey’s post hoc test. Results: The MPv20, MPv50 and MPr20 groups showed significantly higher (p<0.05) bone mineral density on the trabecular bone while all treatment groups recorded significantly higher (p<0.05) tissue mineral density on the cortical bone when compared with OVXC group. Trabecular bone number and separation were significantly higher and lower (p<0.05), respectively, in both MPv20 and MPr20 groups. Significantly higher (p<0.05) cortical bone area fraction and thickness in MPv20 group and medullary area in MPr20 group were observed. Conclusion: Lower dose (20 mg kg-1 day-1) of both leaf and root extracts of Marantodes pumilum var alata preserved bone mineral density and micro-architecture of estrogen-deficient rats better than higher doses.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalInternational Journal of Osteoporosis and Metabolic Disorders
Volume10
Issue number1
DOIs
Publication statusPublished - 2017

Fingerprint

Estrogens
Bone and Bones
Bone Density
Phytoestrogens
Femur
Osteoporosis
Minerals
Analysis of Variance
Tomography
Cancellous Bone
Cortical Bone

Keywords

  • Bone
  • Marantodes pumilum
  • Micro-computed tomography
  • Ovariectomy
  • Phytoestrogens

ASJC Scopus subject areas

  • Internal Medicine
  • Medicine (miscellaneous)
  • Endocrinology, Diabetes and Metabolism

Cite this

@article{23b16979aaef4346b724d98a6c6a18ec,
title = "Low dose Marantodes pumilum leaf and roots extracts preserved bone structure in ovariectomized rats",
abstract = "Background: Marantodes pumilum var. alata, a phytoestrogen-rich herb, has been reported to exert protection on bone of estrogen-deficient animals against osteoporosis. However, comparative osteo-protective activity of its leaf and root extracts has not been fully elucidated. Objective: The aim of this study was to investigate and compare the osteo-protective effects of aqueous leaf and root extracts of Marantodes pumilum var alata in ovariectomized rat model. Methodology: Twenty-seven female rats were divided into nine groups: sham-operated (Sham); ovariectomized control (OVXC); 64.5 μg kg-1 day-1 estrogen treatment (ERT); 20 mg kg-1 day-1 (MPv20), 50 mg kg-1 day-1 (MPv50) and 100 mg kg-1 day-1 (MPv100) doses leaf extract treatments and; 20 mg kg-1 day-1 (MPr20), 50 mg kg-1 day-1 (MPr50) and 100 mg kg-1 day-1 (MPr100) doses root extract treatment groups. After 8 weeks treatment period, the left femora were excised and investigated using Micro-computed tomography (μ-CT). Results were analysed using one-way ANOVA and Tukey’s post hoc test. Results: The MPv20, MPv50 and MPr20 groups showed significantly higher (p<0.05) bone mineral density on the trabecular bone while all treatment groups recorded significantly higher (p<0.05) tissue mineral density on the cortical bone when compared with OVXC group. Trabecular bone number and separation were significantly higher and lower (p<0.05), respectively, in both MPv20 and MPr20 groups. Significantly higher (p<0.05) cortical bone area fraction and thickness in MPv20 group and medullary area in MPr20 group were observed. Conclusion: Lower dose (20 mg kg-1 day-1) of both leaf and root extracts of Marantodes pumilum var alata preserved bone mineral density and micro-architecture of estrogen-deficient rats better than higher doses.",
keywords = "Bone, Marantodes pumilum, Micro-computed tomography, Ovariectomy, Phytoestrogens",
author = "Giaze, {Tijjani Rabiu} and Shuid, {Ahmad Nazrun} and Soelaiman, {Ima Nirwana} and Norliza Muhammad and Norazlina Mohamed",
year = "2017",
doi = "10.3923/ijom.2017.1.9",
language = "English",
volume = "10",
pages = "1--9",
journal = "International Journal of Osteoporosis and Metabolic Disorders",
issn = "1994-5442",
publisher = "Asian Network for Scientific Information",
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}

TY - JOUR

T1 - Low dose Marantodes pumilum leaf and roots extracts preserved bone structure in ovariectomized rats

AU - Giaze, Tijjani Rabiu

AU - Shuid, Ahmad Nazrun

AU - Soelaiman, Ima Nirwana

AU - Muhammad, Norliza

AU - Mohamed, Norazlina

PY - 2017

Y1 - 2017

N2 - Background: Marantodes pumilum var. alata, a phytoestrogen-rich herb, has been reported to exert protection on bone of estrogen-deficient animals against osteoporosis. However, comparative osteo-protective activity of its leaf and root extracts has not been fully elucidated. Objective: The aim of this study was to investigate and compare the osteo-protective effects of aqueous leaf and root extracts of Marantodes pumilum var alata in ovariectomized rat model. Methodology: Twenty-seven female rats were divided into nine groups: sham-operated (Sham); ovariectomized control (OVXC); 64.5 μg kg-1 day-1 estrogen treatment (ERT); 20 mg kg-1 day-1 (MPv20), 50 mg kg-1 day-1 (MPv50) and 100 mg kg-1 day-1 (MPv100) doses leaf extract treatments and; 20 mg kg-1 day-1 (MPr20), 50 mg kg-1 day-1 (MPr50) and 100 mg kg-1 day-1 (MPr100) doses root extract treatment groups. After 8 weeks treatment period, the left femora were excised and investigated using Micro-computed tomography (μ-CT). Results were analysed using one-way ANOVA and Tukey’s post hoc test. Results: The MPv20, MPv50 and MPr20 groups showed significantly higher (p<0.05) bone mineral density on the trabecular bone while all treatment groups recorded significantly higher (p<0.05) tissue mineral density on the cortical bone when compared with OVXC group. Trabecular bone number and separation were significantly higher and lower (p<0.05), respectively, in both MPv20 and MPr20 groups. Significantly higher (p<0.05) cortical bone area fraction and thickness in MPv20 group and medullary area in MPr20 group were observed. Conclusion: Lower dose (20 mg kg-1 day-1) of both leaf and root extracts of Marantodes pumilum var alata preserved bone mineral density and micro-architecture of estrogen-deficient rats better than higher doses.

AB - Background: Marantodes pumilum var. alata, a phytoestrogen-rich herb, has been reported to exert protection on bone of estrogen-deficient animals against osteoporosis. However, comparative osteo-protective activity of its leaf and root extracts has not been fully elucidated. Objective: The aim of this study was to investigate and compare the osteo-protective effects of aqueous leaf and root extracts of Marantodes pumilum var alata in ovariectomized rat model. Methodology: Twenty-seven female rats were divided into nine groups: sham-operated (Sham); ovariectomized control (OVXC); 64.5 μg kg-1 day-1 estrogen treatment (ERT); 20 mg kg-1 day-1 (MPv20), 50 mg kg-1 day-1 (MPv50) and 100 mg kg-1 day-1 (MPv100) doses leaf extract treatments and; 20 mg kg-1 day-1 (MPr20), 50 mg kg-1 day-1 (MPr50) and 100 mg kg-1 day-1 (MPr100) doses root extract treatment groups. After 8 weeks treatment period, the left femora were excised and investigated using Micro-computed tomography (μ-CT). Results were analysed using one-way ANOVA and Tukey’s post hoc test. Results: The MPv20, MPv50 and MPr20 groups showed significantly higher (p<0.05) bone mineral density on the trabecular bone while all treatment groups recorded significantly higher (p<0.05) tissue mineral density on the cortical bone when compared with OVXC group. Trabecular bone number and separation were significantly higher and lower (p<0.05), respectively, in both MPv20 and MPr20 groups. Significantly higher (p<0.05) cortical bone area fraction and thickness in MPv20 group and medullary area in MPr20 group were observed. Conclusion: Lower dose (20 mg kg-1 day-1) of both leaf and root extracts of Marantodes pumilum var alata preserved bone mineral density and micro-architecture of estrogen-deficient rats better than higher doses.

KW - Bone

KW - Marantodes pumilum

KW - Micro-computed tomography

KW - Ovariectomy

KW - Phytoestrogens

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