Loss of mRNA surveillance pathways results in widespread protein aggregation

Nur Hidayah Jamar, Paraskevi Kritsiligkou, Chris M. Grant

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Eukaryotic cells contain translation-associated mRNA surveillance pathways which prevent the production of potentially toxic proteins from aberrant mRNA translation events. We found that loss of mRNA surveillance pathways in mutants deficient in nonsense-mediated decay (NMD), no-go decay (NGD) and nonstop decay (NSD) results in increased protein aggregation. We have isolated and identified the proteins that aggregate and our bioinformatic analyses indicates that increased aggregation of aggregation-prone proteins is a general occurrence in mRNA surveillance mutants, rather than being attributable to specific pathways. The proteins that aggregate in mRNA surveillance mutants tend to be more highly expressed, more abundant and more stable proteins compared with the wider proteome. There is also a strong correlation with the proteins that aggregate in response to nascent protein misfolding and an enrichment for proteins that are substrates of ribosome-associated Hsp70 chaperones, consistent with susceptibility for aggregation primarily occurring during translation/folding. We also identified a significant overlap between the aggregated proteins in mRNA surveillance mutants and ageing yeast cells suggesting that translation-dependent protein aggregation may be a feature of the loss of proteostasis that occurs in aged cell populations.

Original languageEnglish
Article number3894
JournalScientific Reports
Volume8
Issue number1
DOIs
Publication statusPublished - 1 Dec 2018

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Messenger RNA
Protein Biosynthesis
Proteins
Cell Aging
Poisons
Eukaryotic Cells
Proteome
Computational Biology
Ribosomes
Yeasts
Population
Protein Aggregates

ASJC Scopus subject areas

  • General

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Loss of mRNA surveillance pathways results in widespread protein aggregation. / Jamar, Nur Hidayah; Kritsiligkou, Paraskevi; Grant, Chris M.

In: Scientific Reports, Vol. 8, No. 1, 3894, 01.12.2018.

Research output: Contribution to journalArticle

Jamar, Nur Hidayah ; Kritsiligkou, Paraskevi ; Grant, Chris M. / Loss of mRNA surveillance pathways results in widespread protein aggregation. In: Scientific Reports. 2018 ; Vol. 8, No. 1.
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