Loop-mediated isothermal amplification for rapid molecular detection of Enterocytozoon bieneusi in faecal specimens

Siti Nur Su’aidah Nasarudin, Nurul Shazalina Zainudin, Mekadina Bernadus, Azmawati Mohammed Nawi, Alfizah Hanafiah, Emelia Osman

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A loop-mediated isothermal amplification (LAMP) assay was developed to detect Enterocytozoon bieneusi DNA for the first time from human faecal specimens. Four primers specific for Enterocytozoon bieneusi were designed corresponding to small subunit rRNA gene sequences and tested on 100 human faecal specimens. Thirty-nine of the faecal specimens (39 %) were confirmed positive for Enterocytozoon bieneusi by LAMP compared with 33 % by PCR and 32 % by light microscopy. LAMP yielded 94 % sensitivity and 88 % specificity compared with microscopy (sensitivity 48 %, specificity 76 %). No significant differences in positive detection of Enterocytozoon bieneusi were found among the three methods (P>0.05). However, LAMP has shown a substantial agreement with PCR (κ=0.78) and fair agreement was demonstrated between microscopy and PCR (κ=0.25). In conclusion, the LAMP assay proved to be useful as a simplified, rapid, sensitive and specific alternative molecular screening tool in the diagnosis of Enterocytozoon bieneusi in faecal specimens.

Original languageEnglish
Pages (from-to)1329-1334
Number of pages6
JournalJournal of Medical Microbiology
Volume64
Issue number11
DOIs
Publication statusPublished - 1 Nov 2015

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Enterocytozoon
Microscopy
Polymerase Chain Reaction
Sensitivity and Specificity
rRNA Genes
Light
DNA

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

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Loop-mediated isothermal amplification for rapid molecular detection of Enterocytozoon bieneusi in faecal specimens. / Nasarudin, Siti Nur Su’aidah; Zainudin, Nurul Shazalina; Bernadus, Mekadina; Mohammed Nawi, Azmawati; Hanafiah, Alfizah; Osman, Emelia.

In: Journal of Medical Microbiology, Vol. 64, No. 11, 01.11.2015, p. 1329-1334.

Research output: Contribution to journalArticle

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