Long-term administration of tocotrienols and tumor-marker enzyme activities during hepatocarcinogenesis in rats

A. Rahmat, W. Z W Ngah, N. A. Shamaan, A. Gapor, K. A. Kadir

    Research output: Contribution to journalArticle

    39 Citations (Scopus)

    Abstract

    The effects of long-term administration of tocotrienol on hepatocarcinogenesis in rats induced by diethylnitrosamine (DEN) and 2- acetylaminofluorene (AAF) were investigated by determining the activities of γ-glutamyl transpeptidase (GGT), alkaline phosphatase (ALP), glutathione S- transferases (GSTs), and glutathione (GSH) levels in blood and liver. Twenty- eight male 7- to 8-wk-old Rattus norwegicus rats, weighing 120-160 g, were used in this study. The rats were divided into four treatment groups: a control group on a basal diet, a group fed a basal diet supplemented with tocotrienol (30 mg/kg food), a group treated with DEN/AAF, and a group treated with DEN/AAF and fed a diet supplemented with tocotrienol (30 mg/kg food). Blood was collected monthly, and GGT, ALP, and GSH levels were determined. The rats were killed after 9 mo, and the livers were examined morphologically. Grayish white nodules (2/liver) were found in all the DEN/AAF-treated rats (n = 10), but only one of the rats treated with DEN/AAF and supplemented with tocotrienol (n = 6) had liver nodules. A significant increase in the level of blood and liver GSH, ALP, and GGT activities was observed in the DEN/AAF-treated rats. Liver GSTs were similarly increased with DEN/AAF treatment. Tocotrienol supplementation attenuated the impact of the carcinogens in the rats.

    Original languageEnglish
    Pages (from-to)229-232
    Number of pages4
    JournalNutrition
    Volume9
    Issue number3
    Publication statusPublished - 1993

    Fingerprint

    Tocotrienols
    Tumor Biomarkers
    2-Acetylaminofluorene
    Diethylnitrosamine
    Enzymes
    Liver
    Alkaline Phosphatase
    Diet
    Glutathione Transferase
    Food
    gamma-Glutamyltransferase
    Carcinogens
    Glutathione

    ASJC Scopus subject areas

    • Medicine (miscellaneous)
    • Endocrinology, Diabetes and Metabolism
    • Surgery

    Cite this

    Rahmat, A., Ngah, W. Z. W., Shamaan, N. A., Gapor, A., & Kadir, K. A. (1993). Long-term administration of tocotrienols and tumor-marker enzyme activities during hepatocarcinogenesis in rats. Nutrition, 9(3), 229-232.

    Long-term administration of tocotrienols and tumor-marker enzyme activities during hepatocarcinogenesis in rats. / Rahmat, A.; Ngah, W. Z W; Shamaan, N. A.; Gapor, A.; Kadir, K. A.

    In: Nutrition, Vol. 9, No. 3, 1993, p. 229-232.

    Research output: Contribution to journalArticle

    Rahmat, A, Ngah, WZW, Shamaan, NA, Gapor, A & Kadir, KA 1993, 'Long-term administration of tocotrienols and tumor-marker enzyme activities during hepatocarcinogenesis in rats', Nutrition, vol. 9, no. 3, pp. 229-232.
    Rahmat, A. ; Ngah, W. Z W ; Shamaan, N. A. ; Gapor, A. ; Kadir, K. A. / Long-term administration of tocotrienols and tumor-marker enzyme activities during hepatocarcinogenesis in rats. In: Nutrition. 1993 ; Vol. 9, No. 3. pp. 229-232.
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