Localization of genes encoding metallothionein-like protein (mt2 and smtb) in the brain of zebrafish

Seong Lin Teoh, Satoshi Ogawa, Ishwar S. Parhar

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Metallothionein (MT) is a small cysteine-rich heavy metal-binding protein involved in metal homeostasis, detoxification and free radical-scavenging. MT is ubiquitously expressed in several tissues, but its role in the central nervous system is not well understood. In this study, we identified two MT homologous genes (. mt2 and smtb) in the zebrafish. Digoxigenin-. in situ hybridization showed the expression of mt2 and smtb genes in the ventricular layers in the telencephalon, diencephalon, mesencephalon and rhombencephalon, most of which are cell proliferating regions in the brain of zebrafish. Cellular characteristics of MT genes expressing cells were examined by double-labelling with markers for neurons (HuC/D) and astrocytes (glial fibrillary acidic protein, GFAP and S100 protein) and cell proliferation marker (PCNA). mt2 and smtb mRNAs are expressed in neurons and not in astrocytes, and they were co-localized with PCNA. These results suggest that mt2 and smtb may play an important role in neurogenesis and neuroprotection.

Original languageEnglish
Pages (from-to)20-32
Number of pages13
JournalJournal of Chemical Neuroanatomy
Volume70
DOIs
Publication statusPublished - 1 Dec 2015
Externally publishedYes

Fingerprint

Metallothionein
Zebrafish
Proliferating Cell Nuclear Antigen
Brain
Astrocytes
Genes
Proteins
Neurons
Digoxigenin
Diencephalon
Telencephalon
Rhombencephalon
S100 Proteins
Glial Fibrillary Acidic Protein
Neurogenesis
Mesencephalon
Heavy Metals
Free Radicals
In Situ Hybridization
Cysteine

Keywords

  • GFAP
  • HuC/D
  • Neurogenesis
  • Neuroprotection
  • Teleost

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Localization of genes encoding metallothionein-like protein (mt2 and smtb) in the brain of zebrafish. / Teoh, Seong Lin; Ogawa, Satoshi; Parhar, Ishwar S.

In: Journal of Chemical Neuroanatomy, Vol. 70, 01.12.2015, p. 20-32.

Research output: Contribution to journalArticle

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