Liver heme oxygenase-1 expression is positively induced by palm oil-derived tocotrienol-rich fraction (TRF) supplementation in mice

Azman Abdullah, Ahmed Atia, Nadia Salem Alrawaiq

Research output: Contribution to journalArticle

Abstract

The antioxidant activities of tocotrienols are more robust than that of tocopherols. Palm oil is a rich natural source of tocotrienols. Heme oxygenase-1 (HO-1) is an enzyme that has antioxidant, antiinflammatory and cytoprotective functions. The objective of this study is to determine the effects of different doses of TRF oral supplementation on HO-1 gene and protein expression in mice livers. Thirty male ICR white mice (25-30 g) were divided into five groups; three groups were administered TRF orally for 14 days at doses of 200, 500 and 1000 mg/kg respectively (n = 6 per group), a positive control group was administered 100 mg/kg butylated hydroxyanisole (BHA) orally for 14 days (n = 6), and a control group (n = 6) was only administered the vehicle (corn oil). At day 15, the mice were sacrificed and their livers isolated. Total RNA was extracted from the livers and quantitative real-time polymerase chain reaction (qPCR) assays were performed to analyse HO-1 gene expression. The livers were then homogenized and HO-1 protein expression was analysed by Western blotting. It was observed that TRF oral supplementation atconcentrations of 200, 500 and 1000 mg/kg for 14 days resulted in significant concentration-dependentincrease in HO-1 gene and protein expression in mice livers, compared to controls. In conclusion, TRFsupplementation induced HO-1 gene and protein expression in mice livers dose-dependently, with the highest expression seen in mice receiving 1000 mg/kg TRF.

Original languageEnglish
Pages (from-to)55-62
Number of pages8
JournalCurrent Topics in Pharmacology
Volume21
Publication statusPublished - 1 Jan 2017

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Tocotrienols
Heme Oxygenase-1
Liver
Gene Expression
Proteins
Antioxidants
Butylated Hydroxyanisole
Inbred ICR Mouse
Control Groups
Corn Oil
Tocopherols
palm oil
Real-Time Polymerase Chain Reaction
Anti-Inflammatory Agents
Western Blotting
RNA
Enzymes

Keywords

  • Gene expression
  • Heme oxygenase-1
  • Liver
  • Mice
  • Protein expression
  • TRF

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)

Cite this

Liver heme oxygenase-1 expression is positively induced by palm oil-derived tocotrienol-rich fraction (TRF) supplementation in mice. / Abdullah, Azman; Atia, Ahmed; Alrawaiq, Nadia Salem.

In: Current Topics in Pharmacology, Vol. 21, 01.01.2017, p. 55-62.

Research output: Contribution to journalArticle

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