Lipid lowering effect of antioxidant alpha-lipoic acid in experimental atherosclerosis

Zulkhairi Amom, Zaiton Zakaria, Jamaludin Mohamed, Azrina Azlan, Hasnah Bahari, Mohd Taufik Hidayat Baharuldin, Mohd Aris Moklas, Khairul Osman, Zanariyah Asmawi, Mohd Kamal Nik Hassan

Research output: Contribution to journalArticle

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Abstract

Accumulating data demonstrated that hypercholesterolemia and oxidative stress play an important role in the development of atherosclerosis. In the present study, a protective activity of alpha-lipoic acid; a metabolic antioxidant in hypercholesterolemic-induced animals was investigated. Eighteen adult male New Zealand White (NZW) rabbit were segregated into three groups labelled as group N, HCD and ALA (n = 6). Group N (normal control) was fed with normal chow, the rest (HCD and ALA) were fed with 100 g/head/day of 1% cholesterol rich diet to induce hypercholesterolemia. Four point two mg/body weight of alpha lipoic acid was concomintantly supplemented to the ALA group. Drinking water was given ad-libitum. The study was designed for 10 weeks. Blood sampling was taken from the ear lobe vein at the beginning, week 5 and week 10. Plasma was prepared for lipid profile estimation and microsomal lipid peroxidation index indicated with malondialdehyde (MDA) formation. At the end of the experiment, the animals were sacrificed and the aorta were excised for intimal lesion analysis. The plasma total cholesterol (TC) and low density lipoprotein (LDL) levels were found to be significantly low in ALA group compared to that of the HCD group (P<0.05). Similarly, low level of MDA (p<0.05) in ALA group was observed compared to that of the HCD group showing a significant reduction of lipid peroxidation activity. Histomorphometric intimal lesion analysis of the aorta showing less of atheromatous plaque formation in alpha lipoic acid supplemented group (p<0.05) compared to HCD group. These findings suggested that alpha lipoic acid posses a dual lipid lowering and anti-atherosclerotic properties indicated with low plasma TC and LDL levels and reduction of athero-lesion formation in hypercholesterolemic-induced rabbits.

Original languageEnglish
Pages (from-to)88-94
Number of pages7
JournalJournal of Clinical Biochemistry and Nutrition
Volume43
Issue number2
DOIs
Publication statusPublished - Sep 2008

Fingerprint

Thioctic Acid
Atherosclerosis
Antioxidants
Tunica Intima
Lipids
Hypercholesterolemia
Malondialdehyde
Plasmas
LDL Cholesterol
Lipid Peroxidation
Aorta
Animals
Rabbits
Oxidative stress
Atherosclerotic Plaques
Nutrition
Drinking Water
Ear
Veins
Oxidative Stress

Keywords

  • Alpha lipoic acid
  • Antioxidant
  • Atherosclerosis
  • Intimal lesion
  • Lipid peroxidation

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Lipid lowering effect of antioxidant alpha-lipoic acid in experimental atherosclerosis. / Amom, Zulkhairi; Zakaria, Zaiton; Mohamed, Jamaludin; Azlan, Azrina; Bahari, Hasnah; Baharuldin, Mohd Taufik Hidayat; Moklas, Mohd Aris; Osman, Khairul; Asmawi, Zanariyah; Hassan, Mohd Kamal Nik.

In: Journal of Clinical Biochemistry and Nutrition, Vol. 43, No. 2, 09.2008, p. 88-94.

Research output: Contribution to journalArticle

Amom, Zulkhairi ; Zakaria, Zaiton ; Mohamed, Jamaludin ; Azlan, Azrina ; Bahari, Hasnah ; Baharuldin, Mohd Taufik Hidayat ; Moklas, Mohd Aris ; Osman, Khairul ; Asmawi, Zanariyah ; Hassan, Mohd Kamal Nik. / Lipid lowering effect of antioxidant alpha-lipoic acid in experimental atherosclerosis. In: Journal of Clinical Biochemistry and Nutrition. 2008 ; Vol. 43, No. 2. pp. 88-94.
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AU - Baharuldin, Mohd Taufik Hidayat

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