Light-harvesting chlorophyll A/B binding protein (LHCB) promoter for targeting specific expression in oil palm leaves

P. L. Chan, A. Siti Nor Akmar, Roohaida Othman

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Oil palm leaves have the potential to he manipulated as a green factory to produce novel metaholites. In order to direct the specific expression of transgenes into the leaf tissue, a leaf-specific promoter is required. In these studies, efforts were carried out to isolate a leaf-specific gene and its regulatory sequence from the oil palm. Rapid amplification of 5'-cDNA Ends (5'-RACE) resulted in isolation of a 962 hpfull length sequence of the light-harvesting chlorophyll a/h binding protein (LHCB). The amino acid sequence of this gene exhibited more than 88% homology with the LHCBI gene isolated from monocots and dicots. Characterization of this transcript using Northern blot analysis revealed LHCB to he abundantly expressed in oil palm green leaf tissues, but not in the non-photosynthetic tissues such as kernel, mesocarp, germinated seedlings and inflorescences. Results from Southern blot analysis confirmed that at least four copies of the LHCB gene are present in the oil palm genome. The promoter region of LHCB was then obtained through genome walking approach. Based on the sequence analysis, it was found that the LHCB promoter lacks a TA TA-box. Initiation of transcription can therefore be replaced by an initiator element (Inr) located at positions-Ito-7. Furthermore, a few putative ci s-acting elements responsive to light, wounding, abscisic acid and heat-shock were also found in the distal and proximal regions of the LHCB promote.

Original languageEnglish
Pages (from-to)21-29
Number of pages9
JournalJournal of Oil Palm Research
Issue numberSPEC. ISS. JULY
Publication statusPublished - Jul 2008

Fingerprint

Palm oil
Elaeis guineensis
Chlorophyll
binding proteins
Carrier Proteins
Oils
Genes
promoter regions
chlorophyll
Light
leaves
Chlorophyll Binding Proteins
Genome
Tissue
Inflorescence
genes
Abscisic Acid
genome walking
Regulator Genes
Southern Blotting

Keywords

  • Leaf-specific promoter
  • Light-harvesting chlorophyll a/b binding protein (LHCB)
  • Oil palm genetic engineering

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Food Science
  • Renewable Energy, Sustainability and the Environment
  • Biomaterials

Cite this

Light-harvesting chlorophyll A/B binding protein (LHCB) promoter for targeting specific expression in oil palm leaves. / Chan, P. L.; Siti Nor Akmar, A.; Othman, Roohaida.

In: Journal of Oil Palm Research, No. SPEC. ISS. JULY, 07.2008, p. 21-29.

Research output: Contribution to journalArticle

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