Ligation-based mutation detection and RCA in surface un-modified OSTE+ polymer microfluidic chambers

Farizah Saharil, Annika Ahlford, Malte Kuhnemund, Maciej Skolimowski, Alvaro Conde, Martin Dufva, Mats Nilsson, Monica Brivio, Wouter Van Der Wijngaart, Tommy Haraldsson

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

For the first time, we demonstrate DNA mutation detection in surface un-modified polymeric microfluidic chambers without suffering from bubble trapping or bubble formation. Microfluidic devices were manufactured in off-stoichiometry thiol-ene epoxy (OSTE+) polymer using an uncomplicated and rapid fabrication scheme. The device performance was compared to that of PMMA and PDMS devices. In OSTE+ devices, we were able to perform ligation-based mutation detection and rolling circle amplification (RCA) assays directly on the un-modified surface without suffering from bubble formation or enzyme inhibition during bio-operation at elevated temperatures. In contrast, PMMA, PDMS and COP microfluidic devices required specific surface treatment.

Original languageEnglish
Title of host publication2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013
Pages357-360
Number of pages4
DOIs
Publication statusPublished - 2013
Externally publishedYes
Event2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013 - Barcelona
Duration: 16 Jun 201320 Jun 2013

Other

Other2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013
CityBarcelona
Period16/6/1320/6/13

Fingerprint

Microfluidics
Amplification
Bubble formation
Polymers
Enzyme inhibition
Bubbles (in fluids)
Stoichiometry
Surface treatment
Assays
DNA
Fabrication
Temperature

Keywords

  • epoxy
  • lab-on-a-chip
  • microfluidics
  • Molecular diagnostics
  • off-stoichiometry thiol-ene
  • OSTE+
  • polymer
  • RCA
  • rolling circle amplification

ASJC Scopus subject areas

  • Hardware and Architecture
  • Electrical and Electronic Engineering

Cite this

Saharil, F., Ahlford, A., Kuhnemund, M., Skolimowski, M., Conde, A., Dufva, M., ... Haraldsson, T. (2013). Ligation-based mutation detection and RCA in surface un-modified OSTE+ polymer microfluidic chambers. In 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013 (pp. 357-360). [6626776] https://doi.org/10.1109/Transducers.2013.6626776

Ligation-based mutation detection and RCA in surface un-modified OSTE+ polymer microfluidic chambers. / Saharil, Farizah; Ahlford, Annika; Kuhnemund, Malte; Skolimowski, Maciej; Conde, Alvaro; Dufva, Martin; Nilsson, Mats; Brivio, Monica; Van Der Wijngaart, Wouter; Haraldsson, Tommy.

2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013. 2013. p. 357-360 6626776.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Saharil, F, Ahlford, A, Kuhnemund, M, Skolimowski, M, Conde, A, Dufva, M, Nilsson, M, Brivio, M, Van Der Wijngaart, W & Haraldsson, T 2013, Ligation-based mutation detection and RCA in surface un-modified OSTE+ polymer microfluidic chambers. in 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013., 6626776, pp. 357-360, 2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013, Barcelona, 16/6/13. https://doi.org/10.1109/Transducers.2013.6626776
Saharil F, Ahlford A, Kuhnemund M, Skolimowski M, Conde A, Dufva M et al. Ligation-based mutation detection and RCA in surface un-modified OSTE+ polymer microfluidic chambers. In 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013. 2013. p. 357-360. 6626776 https://doi.org/10.1109/Transducers.2013.6626776
Saharil, Farizah ; Ahlford, Annika ; Kuhnemund, Malte ; Skolimowski, Maciej ; Conde, Alvaro ; Dufva, Martin ; Nilsson, Mats ; Brivio, Monica ; Van Der Wijngaart, Wouter ; Haraldsson, Tommy. / Ligation-based mutation detection and RCA in surface un-modified OSTE+ polymer microfluidic chambers. 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013. 2013. pp. 357-360
@inproceedings{5ab6ac5ea09e4ee8bacb1edbc5530ea0,
title = "Ligation-based mutation detection and RCA in surface un-modified OSTE+ polymer microfluidic chambers",
abstract = "For the first time, we demonstrate DNA mutation detection in surface un-modified polymeric microfluidic chambers without suffering from bubble trapping or bubble formation. Microfluidic devices were manufactured in off-stoichiometry thiol-ene epoxy (OSTE+) polymer using an uncomplicated and rapid fabrication scheme. The device performance was compared to that of PMMA and PDMS devices. In OSTE+ devices, we were able to perform ligation-based mutation detection and rolling circle amplification (RCA) assays directly on the un-modified surface without suffering from bubble formation or enzyme inhibition during bio-operation at elevated temperatures. In contrast, PMMA, PDMS and COP microfluidic devices required specific surface treatment.",
keywords = "epoxy, lab-on-a-chip, microfluidics, Molecular diagnostics, off-stoichiometry thiol-ene, OSTE+, polymer, RCA, rolling circle amplification",
author = "Farizah Saharil and Annika Ahlford and Malte Kuhnemund and Maciej Skolimowski and Alvaro Conde and Martin Dufva and Mats Nilsson and Monica Brivio and {Van Der Wijngaart}, Wouter and Tommy Haraldsson",
year = "2013",
doi = "10.1109/Transducers.2013.6626776",
language = "English",
isbn = "9781467359818",
pages = "357--360",
booktitle = "2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013",

}

TY - GEN

T1 - Ligation-based mutation detection and RCA in surface un-modified OSTE+ polymer microfluidic chambers

AU - Saharil, Farizah

AU - Ahlford, Annika

AU - Kuhnemund, Malte

AU - Skolimowski, Maciej

AU - Conde, Alvaro

AU - Dufva, Martin

AU - Nilsson, Mats

AU - Brivio, Monica

AU - Van Der Wijngaart, Wouter

AU - Haraldsson, Tommy

PY - 2013

Y1 - 2013

N2 - For the first time, we demonstrate DNA mutation detection in surface un-modified polymeric microfluidic chambers without suffering from bubble trapping or bubble formation. Microfluidic devices were manufactured in off-stoichiometry thiol-ene epoxy (OSTE+) polymer using an uncomplicated and rapid fabrication scheme. The device performance was compared to that of PMMA and PDMS devices. In OSTE+ devices, we were able to perform ligation-based mutation detection and rolling circle amplification (RCA) assays directly on the un-modified surface without suffering from bubble formation or enzyme inhibition during bio-operation at elevated temperatures. In contrast, PMMA, PDMS and COP microfluidic devices required specific surface treatment.

AB - For the first time, we demonstrate DNA mutation detection in surface un-modified polymeric microfluidic chambers without suffering from bubble trapping or bubble formation. Microfluidic devices were manufactured in off-stoichiometry thiol-ene epoxy (OSTE+) polymer using an uncomplicated and rapid fabrication scheme. The device performance was compared to that of PMMA and PDMS devices. In OSTE+ devices, we were able to perform ligation-based mutation detection and rolling circle amplification (RCA) assays directly on the un-modified surface without suffering from bubble formation or enzyme inhibition during bio-operation at elevated temperatures. In contrast, PMMA, PDMS and COP microfluidic devices required specific surface treatment.

KW - epoxy

KW - lab-on-a-chip

KW - microfluidics

KW - Molecular diagnostics

KW - off-stoichiometry thiol-ene

KW - OSTE+

KW - polymer

KW - RCA

KW - rolling circle amplification

UR - http://www.scopus.com/inward/record.url?scp=84891721243&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84891721243&partnerID=8YFLogxK

U2 - 10.1109/Transducers.2013.6626776

DO - 10.1109/Transducers.2013.6626776

M3 - Conference contribution

SN - 9781467359818

SP - 357

EP - 360

BT - 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013

ER -