Laser capture microdissection with genome-wide expression profiling displayed gene expression signatures in endometrioid endometrial cancer

Norfilza Mohd Mokhtar, Nurul Hanis Ramzi, Wong Yin-Ling, Isa Mohamed Rose, Ahmad Zailani Hatta Mohd Dali, A. Rahman A. Jamal

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

This research determined genes contributing to the pathogenesis of endometrioid endometrial cancer (EEC). Eight pairs of microdissected EEC samples matched with normal glandular epithelium were analyzed using microarray. Unsupervised analysis identified 162 transcripts (58 up- and 104 down-regulated) that were differentially expressed (p < .01, fold change ≥ 1.5) between both groups. Quantitative real-time polymerase chain reaction (qPCR) validated the genes of interest: SLC7A5, SATB1, H19, and ZAK (p < .05). Pathway analysis revealed genes involved in acid amino transport, translation, and chromatin remodeling (p < .05). Laser capture microdissection (LCM) followed by microarray enabled precise assessment of homogeneous cell population and identified putative genes for endometrial carcinogenesis.

Original languageEnglish
Pages (from-to)156-164
Number of pages9
JournalCancer Investigation
Volume30
Issue number2
DOIs
Publication statusPublished - Feb 2012

Fingerprint

Laser Capture Microdissection
Endometrial Neoplasms
Transcriptome
Genome
Large Neutral Amino Acid-Transporter 1
Genes
Chromatin Assembly and Disassembly
Real-Time Polymerase Chain Reaction
Carcinogenesis
Epithelium
Amino Acids
Research
Population

Keywords

  • Endometrioid endometrial cancer
  • Gene expression
  • Genomics
  • Laser capture microdissection
  • Microarray technology

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Laser capture microdissection with genome-wide expression profiling displayed gene expression signatures in endometrioid endometrial cancer. / Mohd Mokhtar, Norfilza; Ramzi, Nurul Hanis; Yin-Ling, Wong; Rose, Isa Mohamed; Hatta Mohd Dali, Ahmad Zailani; A. Jamal, A. Rahman.

In: Cancer Investigation, Vol. 30, No. 2, 02.2012, p. 156-164.

Research output: Contribution to journalArticle

Mohd Mokhtar, Norfilza ; Ramzi, Nurul Hanis ; Yin-Ling, Wong ; Rose, Isa Mohamed ; Hatta Mohd Dali, Ahmad Zailani ; A. Jamal, A. Rahman. / Laser capture microdissection with genome-wide expression profiling displayed gene expression signatures in endometrioid endometrial cancer. In: Cancer Investigation. 2012 ; Vol. 30, No. 2. pp. 156-164.
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