Lactobacillus helveticus (ATCC 27558) upregulates Runx2 and Bmp2 and modulates bone mineral density in ovariectomy-induced bone loss rats

Maria Parvaneh, Golgis Karimi, Rosita Jamaluddin, Min Hwei Ng, Ibrahim Zuriati, Sani Ismaila Muhammad

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Purpose: Osteoporosis is one of the major health concerns among the elderly population, especially in postmenopausal women. Many menopausal women over 50 years of age lose their bone density and suffer bone fractures. In addition, many mortality and morbidity cases among the elderly are related to hip fracture. This study aims to investigate the effect of Lactobacillus helveticus (L. helveticus) on bone health status among ovariectomized (OVX) bone loss-induced rats. Methods: The rats were either OVX or sham OVX (sham), then were randomly assigned into three groups, G1: sham, G2: OVX and G3: OVX+L. helveticus (1 mL of 108–109 colony forming units). The supplementation was force-fed to the rats once a day for 16 weeks while control groups were force-fed with demineralized water. Results: L. helveticus upregulated the expression of Runx2 and Bmp2, increased serum osteocalcin, bone volume/total volume and trabecular thickness, and decreased serum C-terminal telopeptide and total porosity percentage. It also altered bone microstructure, as a result increasing bone mineral density and bone strength. Conclusion: Our results indicate that L. helveticus attenuates bone remodeling and consequently improves bone health in OVX rats by increasing bone formation along with bone resorption reduction. This study suggests a potential therapeutic effect of L. helveticus (ATCC 27558) on postmenopausal osteoporosis.

Original languageEnglish
Pages (from-to)1555-1564
Number of pages10
JournalClinical Interventions in Aging
Volume13
DOIs
Publication statusPublished - 1 Jan 2018

Fingerprint

Lactobacillus helveticus
Ovariectomy
Bone Density
Up-Regulation
Bone and Bones
Postmenopausal Osteoporosis
Bone Remodeling
Porosity
Osteocalcin
Bone Fractures
Health
Hip Fractures
Therapeutic Uses
Bone Resorption
Serum
Osteogenesis
Health Status
Osteoporosis
Stem Cells
Morbidity

Keywords

  • Bone loss
  • Bone mineral density
  • Lactobacillus helveticus
  • Osteoporosis
  • Ovariectomized rats

ASJC Scopus subject areas

  • Geriatrics and Gerontology

Cite this

Lactobacillus helveticus (ATCC 27558) upregulates Runx2 and Bmp2 and modulates bone mineral density in ovariectomy-induced bone loss rats. / Parvaneh, Maria; Karimi, Golgis; Jamaluddin, Rosita; Ng, Min Hwei; Zuriati, Ibrahim; Muhammad, Sani Ismaila.

In: Clinical Interventions in Aging, Vol. 13, 01.01.2018, p. 1555-1564.

Research output: Contribution to journalArticle

Parvaneh, Maria ; Karimi, Golgis ; Jamaluddin, Rosita ; Ng, Min Hwei ; Zuriati, Ibrahim ; Muhammad, Sani Ismaila. / Lactobacillus helveticus (ATCC 27558) upregulates Runx2 and Bmp2 and modulates bone mineral density in ovariectomy-induced bone loss rats. In: Clinical Interventions in Aging. 2018 ; Vol. 13. pp. 1555-1564.
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abstract = "Purpose: Osteoporosis is one of the major health concerns among the elderly population, especially in postmenopausal women. Many menopausal women over 50 years of age lose their bone density and suffer bone fractures. In addition, many mortality and morbidity cases among the elderly are related to hip fracture. This study aims to investigate the effect of Lactobacillus helveticus (L. helveticus) on bone health status among ovariectomized (OVX) bone loss-induced rats. Methods: The rats were either OVX or sham OVX (sham), then were randomly assigned into three groups, G1: sham, G2: OVX and G3: OVX+L. helveticus (1 mL of 108–109 colony forming units). The supplementation was force-fed to the rats once a day for 16 weeks while control groups were force-fed with demineralized water. Results: L. helveticus upregulated the expression of Runx2 and Bmp2, increased serum osteocalcin, bone volume/total volume and trabecular thickness, and decreased serum C-terminal telopeptide and total porosity percentage. It also altered bone microstructure, as a result increasing bone mineral density and bone strength. Conclusion: Our results indicate that L. helveticus attenuates bone remodeling and consequently improves bone health in OVX rats by increasing bone formation along with bone resorption reduction. This study suggests a potential therapeutic effect of L. helveticus (ATCC 27558) on postmenopausal osteoporosis.",
keywords = "Bone loss, Bone mineral density, Lactobacillus helveticus, Osteoporosis, Ovariectomized rats",
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AU - Muhammad, Sani Ismaila

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AB - Purpose: Osteoporosis is one of the major health concerns among the elderly population, especially in postmenopausal women. Many menopausal women over 50 years of age lose their bone density and suffer bone fractures. In addition, many mortality and morbidity cases among the elderly are related to hip fracture. This study aims to investigate the effect of Lactobacillus helveticus (L. helveticus) on bone health status among ovariectomized (OVX) bone loss-induced rats. Methods: The rats were either OVX or sham OVX (sham), then were randomly assigned into three groups, G1: sham, G2: OVX and G3: OVX+L. helveticus (1 mL of 108–109 colony forming units). The supplementation was force-fed to the rats once a day for 16 weeks while control groups were force-fed with demineralized water. Results: L. helveticus upregulated the expression of Runx2 and Bmp2, increased serum osteocalcin, bone volume/total volume and trabecular thickness, and decreased serum C-terminal telopeptide and total porosity percentage. It also altered bone microstructure, as a result increasing bone mineral density and bone strength. Conclusion: Our results indicate that L. helveticus attenuates bone remodeling and consequently improves bone health in OVX rats by increasing bone formation along with bone resorption reduction. This study suggests a potential therapeutic effect of L. helveticus (ATCC 27558) on postmenopausal osteoporosis.

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