Lab-on-a-chip dielectrophoretic manipulation of beta-2 microglobulin for toxin removal in an artificial kidney

Muhammad Izzuddin Abd Samad, Aminuddin Ahmad Kayani, Ahmad Sabirin Zoolfakar, Azrul Azlan Hamzah, Burhanuddin Yeop Majlis, Muhamad Ramdzan Buyong

Research output: Contribution to journalArticle

Abstract

Background: This paper presents a fundamental study of protein manipulation under the influence of dielectrophoretic (DEP) force for a lab-on-a-chip platform. Objective: Protein manipulation is dependent on the polarisation factor of protein when exposed to an electric field. Therefore the objective of this work is a microfluidic device and measurement system are used to characterise the human beta-2 microglobulin (β2M) protein via lateral attractive forces and vertical repulsive forces by means of DEP responses. Method: The manipulation of the β2M protein was conducted using a microfluidic platform with a tapered DEP microelectrode and the protein concentration was quantified based on a biochemical interaction using an Enzyme-Linked Immunosolvent Assay (ELISA). The protein distribution has been analysed based on the β2M concentration for each microfluidic outlet. Results: At 300 kHz, the protein experienced a negative DEP (nDEP) with of 83.3% protein distribution on the middle microchannel. In contrast, the protein experienced a positive DEP (pDEP) at 1.2 MHz with of 78.7% of protein on the left and right sides of the microchannel. Conclusion: This is concept proved that the tapered DEP microelectrode is capable of manipulating a β2M via particle polarisation, hence making it suitable to be utilised for purifying proteins in biomedical application.

Original languageEnglish
Pages (from-to)40-46
Number of pages7
JournalMicro and Nanosystems
Volume11
Issue number1
DOIs
Publication statusPublished - 1 Jan 2019

Fingerprint

Lab-on-a-chip
Proteins
Microfluidics
Microelectrodes
Microchannels
Polarization
Assays
Enzymes

Keywords

  • Artificial kidney
  • Beta-2-microglobulin
  • Dielectrophoresis
  • End-stage renal disease
  • Hollow fibre membrane
  • Lab on chip

ASJC Scopus subject areas

  • Building and Construction

Cite this

Lab-on-a-chip dielectrophoretic manipulation of beta-2 microglobulin for toxin removal in an artificial kidney. / Samad, Muhammad Izzuddin Abd; Kayani, Aminuddin Ahmad; Zoolfakar, Ahmad Sabirin; Hamzah, Azrul Azlan; Yeop Majlis, Burhanuddin; Buyong, Muhamad Ramdzan.

In: Micro and Nanosystems, Vol. 11, No. 1, 01.01.2019, p. 40-46.

Research output: Contribution to journalArticle

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